A study on regeneration of medicinal plant Polygonatum punctatum Royle ex Kunth via callus induction from leaves and petioles
Author affiliations
DOI:
https://doi.org/10.15625/2615-9023/21150Keywords:
Callus, conservation, in vitro, medicinal, Polygonatum punctatum.Abstract
Polygonatum punctatum Royle ex Kunth is a rare medicinal plant species in Vietnam, with rhizomes used as a tonic for kidney yang deficiency, for treating joint pain, and for pain relief. Overexploitation of natural medicinal plants has driven the need for propagation techniques to meet increasing demand, compensate for habitat degradation, and conserve rare species. In this study, tissue culture techniques were applied to investigate the effects of different growth regulators on the in vitro regeneration of P. punctatum. The results showed that sterilizing young leaf and petiole samples with 0.1% HgCl2 for 6 minutes achieved optimal effectiveness. The MS medium supplemented with 1.5 mg/L BA and 0.5 mg/L 2,4-D was identified as the most suitable for inducing callus formation from young leaves and petioles. The MS medium supplemented with 2.0 mg/L BA and 0.2 mg/L NAA yielded the best results for shoot regeneration, while the 1/2 MS medium supplemented with 1.0 mg/L NAA was appropriate for root formation. The in vitro propagation results of P. punctatum contribute to the conservation and sustainable development of this valuable medicinal plant and aim towards the rapid multiplication of healthy plantlets for commercialization.
Downloads
Metrics
References
Ban N. T., Ly D. T., Tap N., Dung V. V., Thin N. N., Tien V. N., Khoi K. N., 2007. Vietnam Red Data Book Part II: Plants. Publishing House for Science and Technology (In Vietnamese).
Bansal M., Mujib A., Bansal Y., Dewir Y. H., Mendler-Drienyovszki N., 2024. An Efficient In Vitro Shoot Organogenesis and Comparative GC-MS Metabolite Profiling of Gaillardia pulchella Foug. Horticulturae 10: 728. https://doi.org/10.3390/horticul turae10070728
Bing H. Y. N., Vinh T. T., Cong V. K., Tham D. T., Duy N. V., 2023. In vitro regeneration of Renanthera imschootiana Rolfe from protocorm-like body. Academia Journal of Biology, 45(2): 47–59. https://doi.org/10.15625/2615-9023/17155
Bisht S., Bisht N. S., Bhandari S., 2012. In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India. Physiol Mol Biol Plants, 18(1): 89–93. doi: 10.1007/s12298-011-0091-5
Duncan D. B., 1995. Multiple range and multiple F tests. Biometrics, 11(1): 1–42.
Hien V. T., Luan V. Q., Huy N. P., Nam N. B., Vinh B. V. T., Du T. X,. Nhut D. T., 2014. Direct Somatic Embryogenesis from Leaf, Petiole and Rhizome Explant of Panax vietnamensis Ha et Grushv. Academia Journal of Biology, 36(1se): 277–282 (In Vietnamese with English summary).
Hong L. V., Thu T. H., Quy P. T., Thao D. P., Thanh N. T., Khanh P. N., 2017. Determination of molecular markers and regeneration of in vitro shoots of Polygonatum kingianum Coll ex Hemsl. Journal of Science and Technology Thai Nguyên University, 04: 189–193 (In Vietnamese with English summary).
Hu M., Hu Z., Du L., Du J., Luo Q., Xiong J., 2019. Establishment of cell suspension culture of Lonicera japonica Thunb and analysis its major secondary metabolites. Ind Crops Prod, 137: 98–104. doi: 10.1016/j.indcrop.2019.05.024
Khiem D. V., Huyen P. X. , Hang N. T. N. , Hoang N. T. P., 2022. In Vitro Regeneration and Acclimatization of Anthurium scherzerianum Schott Plants. Academia Journal of Biology, 44(3): 99–109. doi: 10.15625/2615-9023/16548
Kim M. B., Park C. G., Yi S., Han B. H., 2014. In vitro micropropagation of Polygonatum odoratum cv. Gungangbeaksea. Korean J. Plant Res., 27(5): 540–545. doi: 10.7732/ kjpr.2014.27.5.540
Lekamge D., Yamamoto S., Morohashi S., Matsumoto T., Hatamoto M., Yamaguchi T., Maki S., 2020. Propagation of Polygonatum macranthum (Maxim.) Koidz. from immature seeds using a new sterilization procedure. Plant Biotechnology, 37: 353–357. https://doi.org/10.5511/plant biotechnology.20.0310a
Luciani G. F., Mary A. K., Pellegrini C., Curvetto N. R., 2006. Effects of explants and growth regulators in garlic callus formation and plant regeneration. Plant Cell Tissue Organ Cult, 87: 139–143.
Mo Y. S., Lu T. F., Qiu Z. H., Zhang H. Y., Sun W. B., 2018. Study on the tissue culture and rapid propagation system of Polygonatum cyrtonema Hua. Mod Chin Med, 20(4): 445–449. DOI:10.13313/j. issn. 1673-4890.20171213004
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Plant Physiol, 15: 473–497. https://doi.org/ 10.1111/j.1399-3054.1962.tb08052.x
Nasrin F., Jaber P., Alireza M. A., Saeideh A. S., 2017. Effects of explant type, growth regulators and light intensity on callus induction and plant regeneration in four ecotypes of Persian shallot (Allium hirtifolium). Sci Hort, 218: 80–86. https://doi.org/10.1016/j.scienta.2016.11.056
Park M. W., Ryu S. H., Lee Y. Y., Song J. M., Kim J. H., Ahn Y. H., Bae K. H., 2018. Callus induction and in vitro plant regeneration of Polygonatum stenophyllum Maxim. J Plant Biotechnol, 45(3): 266–272. https://doi.org/10.5010/ JPB.2018.45.3.266
Qadir J., Singh S., Kour S., Kaloo Z. A., Ganai B. A., 2020. In vitro propagation of Polygonatum verticillatum (L.) All. a threatened medicinal herb through seed explant. J Sci Res, 64 (2): 111–117. https://doi.org/10.37398/JSR.2020.640214
Tiwari T., Chaturvedi P., 2018. Callus induction in Polygonatum verticillatum (L.) All.: An Astavarga medicinal herb. J. Pharmacogn. Phytochem., 7(2): 2671–2674.
Tiwari T., Chaturvedi P., 2024. High frequency in vitro multiplication of Polygonatum verticillatum (L.) All. from rhizome bud explants. Bangladesh J. Bot., 53(1): 203–208. https://doi.org/10.3329/ bjb.v53i1.72286
Vinh T. T., Bing H. Y. N., Tham D. T., Hang N. T. T, Cong V. K., Duy N. V., 2021. Micropropagation of paphiopedilum x dalatense. Journal of Biotechnology, 19(1): 155–163. https://doi.org/10.15625/ 1811-4989/14550 (In Vietnamese with English summary).
Xi Y.-K., Yang M., Meng Q.-H., Huang H.-Y., 2022. Induction of adventitious buds and establishment of an efficient regeneration system in Polygonatum cyrtonema Hua. Acta Sci. Pol. Hortorum Cultus, 21(2): 53–62. https://doi.org/ 10.24326/asphc.2022.2.5
Xuyen N. T., Son D. T., Hien P. T., Giang D. T., Huong N. T., Sam V. H., 2021. Effect of different medium factors on in vitro shoot multiplication of Polygonatum kinggianum. Journal of Vietnam Agricultural Science and Technology, 128: 24–29 (In Vietnamese with English summary).
Yan M. M., Xu C., Kim C. H., Um Y. C., Bah A. A., Guo D. P., 2009. Effects of explant type, culture media and growth regulators on callus induction and plant regeneration of Chinese jiaotou (Allium chinense). Sci Hort, 123: 124–128.
Yang G., Huang L. J., Jiang D., Huang J., Cui C., Li N., 2024. Development of indirect somatic embryogenesis and plant regeneration system with immature embryos of the cultivated traditional Chinese medicinal herb Polygonatum cyrtonema. Ind Crops Prod, 214: 118557. https://doi.org/10.1016/j.indcrop.2024.118557
Zhao Q., Wu C., Wang W., Yuan S., Bao J., Chen F., 2009. In vitro plantlet regeneration system from rhizomes and mannose-binding lectin analysis of Polygonatum cyrtonema Hua. Plant Cell Tiss Organ Cult, 99: 269–275. https://doi.org/10.1007/s11240-009-9600-4
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Dang Thi Tham, Nguyen Thi Thanh Hang, Tran Thai Vinh, Quach Van Hoi, Vu Kim Cong, Nong Van Duy
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
