\(\textit{In vitro}\) regeneration and acclimatization of \(\textit{Anthurium scherzerianum }\)schott plants

Dinh Van Khiem; Phan  Xuan Huyen; Nguyen Thi Thanh Hang; Nguyen Thi Phuong Hoang

doi:10.15625/2615-9023/16548

Author affiliations

Authors

Dinh Van Khiem Tay Nguyen Institute for Scientific Research, VAST, Vietnam
Phan Xuan Huyen Tay Nguyen Institute for Scientific Research, VAST, Vietnam
Nguyen Thi Thanh Hang Tay Nguyen Institute for Scientific Research, VAST, Vietnam
Nguyen Thi Phuong Hoang Tay Nguyen Institute for Scientific Research, VAST, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/16548

Keywords:

Anthurium scherzerianum, callus, root formation, shoot regeneration, ventilation condition

Abstract

This study investigated the effects of different PGRs on in vitro plant regeneration of Anthurium scherzerianum Schott and acclimatization in the ex vitro stage. Depending on the objective of each experiment, the in vitro shoots were induced from callus cultured on a modified Murashige and Skoog (MS) medium containing cytokinins and auxins. Results showed that 90% of leaf explants and 48% of petiole explants produced callus. A half-strength MS medium supplemented with 1.0 mg/l Kinetin (KIN) gave the best result for shoot regeneration and 1.5 mg/l
α-naphthalene acetic acid (NAA) is suitable for rooting of A. scherzerianum. The culture vessel having a hole on the cap gave the best result on the growth of A. scherzerianum plantlets. After 90 days transferred to the greenhouse, in vitro plantlets which were cultivated on a mixed substrate of coconut fiber powder: rice husk ash: compost (6:3:1, respectively) had a better growth (shoot length of 8.88 cm, root length of 3.44 cm) than those on another mixed substrate of coconut fiber powder: compost (9:1, respectively).

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References

Atak C., Çelik O., 2009. Micropropagation of Anthurium andraeanum from leaf explant. Pak. J. Bot., 41(3): 1155−1161

Bejoy M., Sumitha V. R., Anish N. P., 2008. Foliar regeneration in Anthurium andraeanum Hort cv. Agnihothri. Biotech., 7(1): 134−138. https://doi.org/ 10.3923/biotech.2008.134.138

Duncan D. B., 1955. Multiple range and F tests. Biometrics, 11: 1−42. https://doi.org/10.2307/3001478

Cardoso J. C., Habermann G., 2014. Adventitious shoot induction from leaf segments in Anthurium andreanum is affected by age of explant, leaf orientation and plant growth regulator. Hortic. Environ. Biotechnol., 55(1): 56–62. https://doi.org/10.1007/s13580-014-0022-9

Fal M., Majada J., Sánchez Tamés R., 2002. Physical environment in non-ventilated culture vessels affects in vitro growth and morphogenesis of several cultivars of Dianthus Caryophyllus L. In vitro Cellular and Developmental Biology-Plant, 38(6): 589−594.

Gu A., Liu W. F., Ma C., Cui J., Henny R. J., Chen J. J., 2012. Regeneration of Anthurium andraeanum from leaf explants and evaluation of microcutting rooting and growth under different light qualities. HortScience, 47: 88−92. https://doi.org/ 10.21273/HORTSCI.47.1.88

Geier T., 1986. Factors affecting plant regeneration from leaf segments of Anthurium scherzerianum Schott (Araceae) cultured in vitro. Plant Cell Tissue Organ Cult., 6: 115−125.

Geier T., 1990. Anthurium. In: Amirato P. V., Evans D. A., Sharp W. R., Bajaj Y. P. S. (Eds.), Handbook of Plant Cell Culture, Ornamental Species, Vol. 5. McGraw-Hill, New York, pp. 228−252.

Kozai T., Fujiwara K., Watanabe I., 1986. Fundamental studies on environments in plant tissue cultue vessels. (2) Effects of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers. J. Agric. Met., 42(2): 119−127 (in Japanese with English abstract).

Kuehnle A. R., Chen F. C., Sugii N., 1992. Somatic embryogenesis and plant regeneration in Anthurium andraeanum L. hybrids. Plant Cell Rep., 11: 438−442. https://doi.org/10.1007/BF00232686

Kumar S. S., Deth S. K., Seeni S., 1992. Development of floricultural resources in Kerala-rapid micropropagation of Anthuriums. In: Proceedings of the Forth Kerala Science Congress, Thrissur Kerala, pp 347−350.

Jahan M. T., Islam M. R., Khan R., Mamun A. N. K., Ahmed G., Hakim H., 2009. In vitro clonal propagation of Anthurium (Anthurium andraeanum Lind) using callus culture. Plant Tiss Cult Biotech, 19(1): 61−69. https://doi.org/10.3329/ ptcb.v19i1.4961

Joseph D., Martin K. P., Madassery J., Philip V. J., 2003. In vitro propagation of three commercial cut flower cultivars of Anthurium andraeanum Hort. Indian J. Exp. Biol., 41(2): 154−159.

Lightbourn G. J., Deviprasad P. V., 1990. In vitro techniques for rapid multiplication of four varieties of Anthurium andraeanum in Jamaica. Proc Interam Soc Trop Hortic., 34: 3−5.

Liu C. M., Xu Z. H., 1992. An efficient procedure for micropropagation of Anthurium scherzerianum Schott (flamingo flower). Chin. J. Bot., 4: 49−55 (in Chinese with English abstract).

Martin K. P., Joseph D., Madassery J., Philip V. J., 2003. Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort. In Vitro Cellular and Developmental Biology-Plant, 39(5): 500–504. doi:10.1079/IVP2003460.

McCown B. H., Sellmer J. C., 1986. General media and vessels suitable for woody plant culture, Ed, Vol 1 General Principles and Biotechnology. Martinus Nijhoff Publishers, The Netherlands.

Murashige T., Skoog F., 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant., 15: 473−497. https://doi.org/ 10.1111/j.1399-3054.1962.tb08052.x

Musa H., Abdul Ghani A. K., Pierre D., 1997. Somatic embryogenesis and plant regeneration in Anthurium scherzerianum. Plant Cell, Tissue and Organ Culture, 48: 189−193.

Mills D., Yanqing Z., Benzioni A., 2004. Improvement of jojoba shoot multiplication in vitro by ventilation. In Vitro Cellular and Developmental Biology-Plant, 40(4): 396−402. https://doi.org/10.1079/IVP2004537.

Nhut D.T., Duy N., Vy A. N. H., Khue C.D., Khiem D. V., Vinh D. N., 2006. Impact of Anthurium spp. genotype on callus induction derived from leaf explants, shoot and root regeneration capacity from callus. J. Appl. Hort. 8(2): 135−137. https://doi.org/10.37855/jah.2006.v08i02.31

Raad M. K., Zanjani S. B., Shoor M., Hamidoghli Y., Sayyad A. R., Harabian Masouleh A., Kaviani B., 2012. Callus induction and organogenesis capacity from lamina and petiole explants of Anthurium andraeanum Linden (Casino and Antadra). Aust. J. Crop. Sci., 6(5): 928−937.

Puchooa D., Sookun D., 2003. Induced mutation and in vitro cultured of Anthurium andraeanum. AMAS, Food and Agricultural Research Council, Reduit, Mauritius: 17−27. https://doi.org/ 10.1.1.611.7415

Reisch L., 1998. Effect of media on production of Anthurium. Hawaii Agr. Exp. Sta. Prog. Notes No. 94.

Sallanon H., Maziere Y., 1992. Influence of growth room and vessel humidity on the in vitro development of rose plants. Plant Cell, Tissue Organ Cult., 30(2): 121−125.

Te-Chato S., Naksombut S., Boonsiri J., 2002. Effect of variety and explants on callus formation and micropropagation of Anthurium. Songklanakarin J Sci Technol, 24: 569−578.

Te-Chato S., Susanon T., Sontikun Y., 2006. 20 medium influencing embryogenesis and organogenesis in Anthurium spp. cultivar, explants type and culture. Songklanakarin J Sci Technol, 28 (4): 717−722.

Winarto B., Hayati N. A., Purwito A., Marwoto B., 2009. Effect of sucrose and glucose on successful induction and regeneration of callus derived from anther culture of Anthurium. J Biol Res, 14(2): 165−172 (in Indonesian with English abstract).

Winarto B., Hayati N. A., Purwito A., Marwoto B., 2010a. Improvement of selected induction culture media on callus induction in anther culture of Anthurium and a histological study on its callus formation. J. Nat. Indones, 12(2): 93−10 (in Indonesian with English abstract).

Winarto, B., Hayati, N. A., Purwito, A., Marwoto, B., 2010b. Application of 2,4-D and TDZ in formation and regeneration of callus derived from anther culture of Anthurium. J. Hortik., 20(1): 1−9 (in Indonesian with English abstract).