Study on in vitro propagation of \(\textit{Vaccinium myrtillus}\) Linn. via nodal culture method
Author affiliations
DOI:
https://doi.org/10.15625/2615-9023/16546Keywords:
Number of nodes, number of shoots, shoot height, Vaccinium myrtillus, WPM medium.Abstract
Vaccinium myrtillus Linn. is an imported fruit tree with high economic value. Currently, in Vietnam there has not been any publication on in vitro propagation of the blueberry plant. This study is focused on in vitro propagation of V. myrtillus by nodal culture. The results showed that in vitro shoot growth and proliferation were the best when nodes were cultured on WPM medium supplemented with 0.5 mg/L zeatin, 30 g/L sucrose, 10 g/L agar, pH 5.8 (shoot height 4.24 cm,
1 shoot/explant, 8.60 nodes/shoot). The third nodes to sixth nodes proved to be suitable materials for in vitro propagation of V. myrtillus. The proliferation and growth of shoots on the medium without activated charcoal (plant height 3.92 cm, 1 shoot/explant, 8.60 nodes/shoot) were better than those grown on the medium supplemented with 1 g/L of activated charcoal (plant height 3.64 cm, 1 shoot/explant, 7.90 nodes/shoot). For the in vitro rooting stage, the WPM medium containing 1.0 mg/L IBA, 30 g/L sucrose, 10 g/L agar, pH 5.8 resulted in plant height 5.49 cm, root length 1.42 cm, root formation rate 100%. After transferring the cultivated-tissue Vaccinium myrtillus to the greenhouse, substrate with coconut fibre powder, the results were observed to be plant height 6.94 cm, root length 3.18 cm, survival rate 95%. The plantlet growth increased overtime after 4 months in the greenhouse.
Downloads
Metrics
References
Cüce M., Sökmen A., 2015. Micropropagation of Vaccinium myrtillus L. (Bilberry) naturally growing in the Turkish flora. Turk J. Biol., 39: 233–240.
Cüce M., Bekircan T., Sökmen A., 2016. Effect of medium pH on shoot regeneration of three Vaccinium species naturally growing in Turkish Flora. Turk J. Life Sci., 1(2): 052–058.
Cüce M., Sökmen A., 2017. In vitro production protocol of Vaccinium uliginosum L. (bog bilberry) growing in the Turkish flora. Turk J. Agric. For., 41: 294–304.
Duncan D. B., 1955. Multiple range and F tests. Biometrics, 11: 1–42.
Elkiran O, Avsar C., 2020. Chemical composition and biological activities of the essential oil from the leaves of Vaccinium myrtillus L. Bangladesh J. Bot., 49(1): 91–96.
Gajdošová A., Ostrolucká M. G., Libiaková G., Ondrušková E., Šimala D., 2006. Microclonal propagation of Vaccinium sp. and Rubus sp. and detection of genetic variability in culture in vitro. J. Fruit Ornam. Plant Res., 14: 103–119.
Güder A., Gür M., Engin M. S., 2015. Antidiabetic and antioxidant properties of bilberry (Vaccinium myrtillus Linn.) fruit and their chemical composition. J. Agr. Sci. Tech., 17: 401–414.
Jaakola L., Riihinen K., Häggman H., Hohtola A., 2009. Bilberry in vitro protocols and analyses of phenolic compounds. Methods Mol. Biol., 547: 71–80.
Lloyd G., McCown B., 1980. Commercially-feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture. Com. Proc. Int. Plant Prop. Soc., 30: 421–427.
Melani N. A., Adriana P.V., 2016. In vitro propagation of Vaccinium myrtillus. J. N. R. D., 6: 122–129.
Mohamed G. R. A., Khusnetdinova L. Z., Timofeeva O. A., 2018. Elaboration of micropropagation protocol for Vaccinium corymbosum cv. "Sunt Blue Giant". Asian J. Plant Sci. Res., 8(5): 1–11.
Nestby R., Percival D., Martinussen I., Opstad N., Rohloff J., 2010. The European blueberry (Vaccinium myrtillus L.) and the potential for cultivation. A review. European J. Plant Sci. Biotechnol., 5: 5–16.
Nguyen Thi Kim Yen, Nguyen Phuc Huy, Hoang Van Cuong, Nguyen Thi Nhat Linh, Nguyen Ba Nam, Vu Quoc Luan, Duong Tan Nhut, 2013. Effect of activated charcoal and ventilation culture on growth and development of Gerbera jamesonii in vitro and ex vitro. Vietnam J. Sci. Technol., 51(4): 435–446.
Pan M. J., Staden van J., 1998. The use of charcoal in in vitro culture: review, Plant Growth Regul., 26: 155–163.
Phan Xuan Huyen, Nguyen Lam Thanh, 2014. Study on in vitro propagation of Codonopsis javanica Blume via axillary shoot culture. Vietnam J. Biotechnol., 12(4): 659–666.
Phan Xuan Huyen, Nguyen Thi Phuong Hoang, 2017. Study on in vitro shoot regeneration and cultivation of Anoectochilus formosaus Hayata. Vietnam J. Biotechnol., 15(3): 515–524.
Pepkolaj A., Faslia N., Ozuni E., 2017. Chemical variability of wild bilberry (Vaccinium myrtillus L.) populations in northern part of Albania. J. Med. Plants Res., 11(37): 576–582.
Phan Xuan Huyen, Huynh Thi Ngoan, Nguyen Thi Phuong Hoang, 2017. Study on In vitro propagation of Hibicus sagittifolius Kurz through stem node culture. Vietnam Agr. Sci. J., 15(5): 664–672.
Phan Xuan Huyen, Tran Thi Hoang Anh, Nguyen Thi Phuong Hoang, Nguyen Thi Thanh Hang, Dinh Van Khiem, Hoang Van Cuong, 2018. Study on in vitro multiplication and effect of foliar fertilizers on growth of Anoectochilus formosanus Hayata at Lamdong province. Vietnam J. Med. Mater., 23(1): 52–59.
Phan Xuan Huyen, Truong Ngoc Thao Vy, Nguyen Thi Phuong Hoang, Nguyen Thi Thanh Hang, Dinh Van Khiem, 2021. In vitro propagation and growth of ex vitro Chrysanthemum indicum L. in Da Lat-Lam Dong. Vietnam J. Biotechnol., 19(1): 175–184.
Rache L. Y. C., Pacheco J. C. M., 2010. In vitro propagation of mature plants of Vaccinium meridionale (Ericaceae). Acta Bot. Bras., 24(4): 1086–1095.
Vu Quoc Luan, Tran Dinh Phuong, Tran Cong Luan, Duong Tan Nhut, 2015. Micropropagation and determination of β-sitosterol active ingredients on Anoectochilus setaceus Blume. Vietnam J. Biotechnol., 13(4): 1113–1125.
Wann S. R., Veazey R. L., Kaphammer J., 1997. Activated charcoal does not catalyze sucrose hydrolysis in tissue culture media during autoclaving. Plant Cell Tiss. Org. Cult., 50: 221–224.
Webb D. T., Flinn B. S., Georgis W., 1988. Micropropagation of eastern white pine (Pinus strobus L.). Can. J. For. Res., 18: 1570–1580.
Downloads
Published
How to Cite
Issue
Section
