Antioxidant and cytoprotective activities of marine fungi isolated from brown seaweeds in Nha Trang Bay, Khanh Hoa Province, Central Vietnam

Thi Duy Ngoc Ngo; Thi Hoai Trinh Phan; Ekaterina A. Yurchenko; Anton N. Yurchenko; Thi Thuy Hang Cao; Thi Dieu Trang Vo; Thi Thanh Van Tran; Duc Thinh Pham; Nhu Khanh Huynh Hoang; Thi Nam Phuong Bui

doi:10.15625/1859-3097/18869

Author affiliations

Authors

Ngo Thi Duy Ngoc Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Phan Thi Hoai Trinh Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Ekaterina A. Yurchenko G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Russia
Anton N. Yurchenko G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Russia
Cao Thi Thuy Hang Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Vo Thi Dieu Trang Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Tran Thi Thanh Van Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Pham Duc Thinh Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Huynh Hoang Nhu Khanh Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Bui Thi Nam Phuong Graduate University of Sciences and Technology, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/18869

Keywords:

Antioxidant activity, cytoprotective activity, Penicillium, Aspergillus, brown seaweed, marine fungi.

Abstract

This study aims to evaluate the antioxidant and cytoprotective activities of 32 fungal strains isolated from brown seaweeds collected from Nha Trang Bay, Khanh Hoa Province, Central Vietnam. These fungal extracts were screened for antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radical scavenging assay. In contrast, cytoprotective activity on rat cardiomyocytes H9c2 cell line was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. The results indicated the capacity for free DPPH and ABTS radical scavenging of fungal crude extracts with 31.25% (n = 10) and 81.25% (n = 26), respectively. Among the fungal strains with high antioxidant activity, the three fungal extracts 2104NT-1.3, 2104NT-3.3, and 2104NT-7.7 increased the viability of rotenone-exposed cardiomyocyte cells by 9.9%, 15.2%, and 13.6%, respectively. Three fungal strains with significant antioxidant and cytoprotective activities were identified as Penicillium chermesinum 2104NT-1.3, Aspergillus sp. 2104NT-3.3, and Penicillium sp. 2104NT-7.7 based on sequence analysis of internal transcribed spacer (ITS) region. This study provided the potential fungal strains isolated from Nha Trang Bay for further investigation of antioxidant and cytoprotective compounds.

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References

Bugni, T. S., and Ireland, C. M., 2004. Marine-derived fungi: a chemically and biologically diverse group of microorganisms. Natural product reports, 21(1), 143–163.

Hasan, S., Ansari, M. I., Ahmad, A., and Mishra, M., 2015. Major bioactive metabolites from marine fungi: A Review. Bioinformation, 11(4), 176.

Rateb, M. E., and Ebel, R., 2011. Secondary metabolites of fungi from marine habitats. Natural Product Reports, 28(2), 290–344.

Debbab, A., Aly, A. H., and Proksch, P., 2011. Bioactive secondary metabolites from endophytes and associated marine derived fungi. Fungal Diversity, 49(1), 1–12.

Suryanarayanan, T. S., and Johnson, J. A., 2014. Fungal endosymbionts of macroalgae: need for enquiries into diversity and technological potential. Oceanography, 2(119), 1–3.

Suryanarayanan, T. S., 2012. Fungal endosymbionts of seaweeds. In Biology of marine fungi . Berlin, Heidelberg: Springer Berlin Heidelberg. pp. 53–69.

Zhang, P., Li, X., and Wang, B. G., 2016. Secondary metabolites from the marine algal-derived endophytic fungi: Chemical diversity and biological activity. Planta Medica, 82(09/10), 832–842.

Sarasan, M., Puthumana, J., Job, N., Han, J., Lee, J. S., and Philip, R., 2017. Marine Algicolous Endophytic Fungi-A Promising Drug Resource of the Era. Journal of Microbiology and Biotechnology, 27(6), 1039–1052.

Flewelling, A. J., Currie, J., Gray, C. A., and Johnson, J. A., 2015. Endophytes from marine macroalgae: promising sources of novel natural products. Current Science, 108, 88–111.

Dighton, J., and White, J. F., 2005. Fungal Communities of Seaweeds. In The Fungal Community. CRC Press. pp. 553–600.

Girich, E. V., Trinh, P. T. H., Nesterenko, L. E., Popov, R. S., Kim, N. Y., Rasin, A. B., Menchinskaya, E. S., Kuzmich, A. S., Chingizova, E. A., Minin, A. S., Ngoc, N. T. D., Van, T. T. T., Yurchenko, E. A., Yurchenko, A. N., and Berdyshev, D. V., 2023. Absolute stereochemistry and cytotoxic effects of vismione E from marine sponge-derived fungus Aspergillus sp. 1901nt-1.2. 2. International Journal of Molecular Sciences, 24(9), 8150.

Girich, E. V., Yurchenko, A. N., Smetanina, O. F., Trinh, P. T. H., Ngoc, N. T. D., Pivkin, M. V., Popov, R. S., Pislyagin, E. A., Menchinskaya, E. S., Chingizova, E. A., Afiyatullov, S. S., and Yurchenko, E. A., 2020. Neuroprotective metabolites from vietnamese marine derived fungi of Aspergillus and Penicillium genera. Marine drugs, 18(12), 608.

Yurchenko, A. N., Trinh, P. T. H., Girich, E. V., Smetanina, O. F., Rasin, A. B., Popov, R. S., Dyshlovoy, S. A., von Amsberg, G., Menchinskaya, E. S., Van, T. T. T., and Afiyatullov, S. S., 2019. Biologically active metabolites from the marine sediment-derived fungus Aspergillus flocculosus. Marine drugs, 17(10), 579.

Handayani, D., Ornando, R., and Rustini, 2016. Antimicrobial activity screening of symbiotic fungi from marine sponge Petrosia nigrans collected from South Coast of West Sumatera Indonesia. International Journal of Pharmacognosy and Phtochemical research, 8(4), 623–625.

Sobolevskaya, M. P., Leshchenko, E. V., Hoai, T. P., Denisenko, V. A., Dyshlovoy, S. A., Kirichuk, N. N., Khudyakova, Y. V., Kim, N. Y., Berdyshev, D. V., Pislyagin, E. A., Kuzmich, A. S., Gerasimenko, A. V., Popov, R. S., von Amsberg, G., Antonov, A. S., and Afiyatullov, S. S., 2016. Pallidopenillines: Polyketides from the alga-derived fungus Penicillium thomii Maire KMM 4675. Journal of natural products, 79(12), 3031–3038.

Lee, K. J., Oh, Y. C., Cho, W. K., and Ma, J. Y., 2015. Antioxidant and anti‐inflammatory activity determination of one hundred kinds of pure chemical compounds using offline and online screening HPLC assay. Evidence‐Based Complementary and Alternative Medicine, 2015(1), 165457.

Jeong, S. H., Kim, H. K., Song, I. S., Lee, S. J., Ko, K. S., Rhee, B. D., Kim, N., Mishchenko, N. P., Fedoryev, S. A., Stonik, V. A., and Han, J., 2014. Echinochrome A protects mitochondrial function in cardiomyocytes against cardiotoxic drugs. Marine drugs, 12(5), 2922–2936.

Fredricks, D. N., Smith, C., and Meier, A., 2005. Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR. Journal of clinical microbiology, 43(10), 5122–5128.

White, T. J., Bruns, T., Lee, S. J. W. T., and Taylor, J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18(1), 315–322.

Abdel-Lateff, A., Fisch, K. M., Wright, A. D., and König, G. M., 2003. A new antioxidant isobenzofuranone derivative from the algicolous marine fungus Epicoccum sp. Planta medica, 69(09), 831–834.

Li, X., Li, X. M., Xu, G. M., Li, C. S., and Wang, B. G., 2014. Antioxidant metabolites from marine alga-derived fungus Aspergillus wentii EN-48. Phytochemistry letters, 7, 120–123.

Hulikere, M. M., Joshi, C. G., Ananda, D., Poyya, J., and Nivya, T., 2016. Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii). Mycology, 7(4), 203–211.

Trinh, P. T. H., Van, T. T. T., Ngoc, N. T. D., Hang, C. T. T., Hoa, L. T., Trung, D. T., Khanh, H. H. N., and Hung, L. D., 2019. Evaluation of antioxidant and antimicrobial activities of marine fungi isolated from Nha Trang Bay. Journal of Biology, 41(2se1&2se2), 409–417. (in Vietnamese).

Dao, D. Q., Phan, T. T. T., Nguyen, T. L. A., Trinh, P. T. H., Tran, T. T. V., Lee, J. S., Shin, H. J., and Choi, B. K., 2020. Insight into antioxidant and photoprotective properties of natural compounds from marine fungus. Journal of chemical information and modeling, 60(3), 1329–1351.

Phaniendra, A., Jestadi, D. B., and Periyasamy, L., 2015. Free radicals: properties, sources, targets, and their implication in various diseases. Indian Journal of Clinical Biochemistry, 30, 11–26.

Bălașa, A. F., Chircov, C., and Grumezescu, A. M., 2020. Marine biocompounds for neuroprotection—A review. Marine Drugs, 18(6), 290.

Yurchenko, E. A., Menchinskaya, E. S., Pislyagin, E. A., Trinh, P. T. H., Ivanets, E. V., Smetanina, O. F., and Yurchenko, A. N., 2018. Neuroprotective activity of some marine fungal metabolites in the 6-hydroxydopamin-and paraquat-induced Parkinson’s disease models. Marine drugs, 16(11), 457.

Trinh, P. T. H., Girich, E. V., Yurchenko, A. N., Khmel, O. O., Dieu, T. V. T., Ngoc, N. T. D., Lee, J. S., Menshov, A. S., Kim, N. Y., Chingizova, E. A., Van, T. T. T., Lee, H. S., and Yurchenko, E. A., 2022. Cytoprotective polyketides from sponge-derived fungus Lopadostoma pouzarii. Molecules, 27(21), 7650.

Imhoff, J. F., 2016. Natural products from marine fungi—Still an underrepresented resource. Marine drugs, 14(1), 19.

Nicoletti, R., and Vinale, F., 2018. Bioactive compounds from marine-derived Aspergillus, Penicillium, Talaromyces and Trichoderma species. Marine drugs, 16(11), 408.

Trinh, P. T. H., Chanh, N. T. K., Ngoc, N. T. D., Tien, P. Q., Ly, B. M., and Van, T. T. T., 2017. Secondary metabolites from a marine-derived fungus Penicillium chrysogenum 045-357-2. Vietnam Journal of Science and Technology, 55(1A), 65–72.

Choi, B.-K., Park, S.-Y., Jo, S.-H., Choi, D.-K., Trinh, P. T. H., Lee, H.-S., Cao, V. A., Ngo, T. D. N., Van, T. T. T., and Shin, H. J., 2020. Restricticin B from the marine fungus Penicillium janthinellum and its inhibitory activity on the NO production in BV-2 microglia cells. Preprints, 2020020139.

Yurchenko, A. N., Smetanina, O. F., Ivanets, E. V., Phan, T. T. H., Ngo, N. T. D., Zhuravleva, O. I., Rasin, A. B., Dyshlovoy, S. A., Menchinskaya, E. S., Pislyagin, E. A., Amsberg, G. V., Afiyatullov, S. S., and Yurchenko, E. A., 2020. Auroglaucin-related neuroprotective compounds from Vietnamese marine sediment-derived fungus Aspergillus niveoglaucus. Natural product research, 34(18), 2589–2594.