Antioxidant and cytoprotective activities of marine fungi isolated from brown seaweeds in Nha Trang Bay, Khanh Hoa Province, Central Vietnam

Thi Duy Ngoc Ngo; Thi Hoai Trinh Phan; Ekaterina A. Yurchenko; Anton N. Yurchenko; Thi Thuy Hang Cao; Thi Dieu Trang Vo; Thi Thanh Van Tran; Duc Thinh Pham; Nhu Khanh Huynh Hoang; Thi Nam Phuong Bui

doi:10.15625/1859-3097/18869

Author affiliations

Authors

Ngo Thi Duy Ngoc Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Phan Thi Hoai Trinh Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Ekaterina A. Yurchenko G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Russia
Anton N. Yurchenko G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Russia
Cao Thi Thuy Hang Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Vo Thi Dieu Trang Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Tran Thi Thanh Van Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Pham Duc Thinh Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Huynh Hoang Nhu Khanh Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Bui Thi Nam Phuong Graduate University of Sciences and Technology, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/18869

Keywords:

Antioxidant activity, cytoprotective activity, Penicillium, Aspergillus, brown seaweed, marine fungi.

Abstract

This study aims to evaluate the antioxidant and cytoprotective activities of 32 fungal strains isolated from brown seaweeds collected from Nha Trang Bay, Khanh Hoa Province, Central Vietnam. These fungal extracts were screened for antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radical scavenging assay. In contrast, cytoprotective activity on rat cardiomyocytes H9c2 cell line was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. The results indicated the capacity for free DPPH and ABTS radical scavenging of fungal crude extracts with 31.25% (n = 10) and 81.25% (n = 26), respectively. Among the fungal strains with high antioxidant activity, the three fungal extracts 2104NT-1.3, 2104NT-3.3, and 2104NT-7.7 increased the viability of rotenone-exposed cardiomyocyte cells by 9.9%, 15.2%, and 13.6%, respectively. Three fungal strains with significant antioxidant and cytoprotective activities were identified as Penicillium chermesinum 2104NT-1.3, Aspergillus sp. 2104NT-3.3, and Penicillium sp. 2104NT-7.7 based on sequence analysis of internal transcribed spacer (ITS) region. This study provided the potential fungal strains isolated from Nha Trang Bay for further investigation of antioxidant and cytoprotective compounds.

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