Intracellular reactive oxygen species scavenging effect of fucosterol isolated from the brown alga <i>Sargassum crassifolium</i> in Vietnam

Hoang Kim Chi; Le Huu Cuong; Nguyen Thi Hong Van; Tran Thi Nhu Hang; Do Huu Nghi; Tran Mai Duc; Le Mai Huong; Tran Thi Hong Ha

doi:10.15625/1859-3097/20/3/15248

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Authors

Hoang Kim Chi Institute of Natural Products Chemistry, VAST, Vietnam Graduate University of Science and Technology, VAST, Vietnam
Le Huu Cuong Institute of Natural Products Chemistry, VAST, Vietnam
Nguyen Thi Hong Van Institute of Natural Products Chemistry, VAST, Vietnam
Tran Thi Nhu Hang Institute of Natural Products Chemistry, VAST, Vietnam
Do Huu Nghi Institute of Natural Products Chemistry, VAST, Vietnam
Tran Mai Duc Nha Trang Institute of Technology Research and Application, VAST, Vietnam
Le Mai Huong Institute of Natural Products Chemistry, VAST, Vietnam
Tran Thi Hong Ha Institute of Natural Products Chemistry, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/20/3/15248

Keywords:

Sargassum crassifolium, alga, fucosterol, ROS, antioxidant, Hep-G2 cells.

Abstract

Sargassum is a widely distributed marine brown algal genus in Vietnam and has been considered a source of diverse bioactive metabolites. In this study, S. crassifolium collected from South Central region of Vietnam was chemically studied and bioactively evaluated. Fucosterol was isolated and identified from the methanolic extract of the alga by means of chemical fractionation and spectral analysis and showed no cytotoxic effect in Hep-G2 cells at the observed concentrations. In vitro assay for intracellular reactive oxygen species by dichlorofluorescein method revealed a potent scavenging effect of the isolated compound. Accordingly, the level of intracellular reactive oxygen species induced by hydrogen peroxide (H₂O₂) was reduced by 71.66% with the treatment of fucosterol at 10 µg.ml^-1. The results indicated the ability of algal fucosterol in diffusing into cells and preventing the production of different ROS compounds and further suggested the therapeutic potential against diseases caused by oxidative stress of natural metabolites from S. crassifolium in Vietnam.

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