Improvement of <i>in vitro</i> shoot regeneration and flowering from petal explants of <i>Begonia X hiemalis</i> fotsch

Vu Quoc Luan; Duong Tan Nhut

doi:10.15625/vjbt-17561

Author affiliations

Authors

Vu Quoc Luan \(^1\) Taynguyen Institute for Scientific Research, VAST, Dalat, 670000, Vietnam https://orcid.org/0009-0004-7449-2826
Duong Tan Nhut \(^1\) Taynguyen Institute for Scientific Research, VAST, Dalat, 670000, Vietnam

DOI:

https://doi.org/10.15625/vjbt-17561

Keywords:

Begonia x hiemalis Fotsch, inflorescences, myo-inositol, petal explants, red-leaf

Abstract

Begonias hold significant importance due to their use as culinary plants, medicinal remedies, decorative elements, and high-value crops. This research aims to find new explant materials for regeneration shoots and direct flowering with high frequency. In this study, high-frequency shoot regeneration and in vitro flowering from petal explants of Begonia x hiemalis Fotsch were achieved. Whole petal explants disinfected with an AgNPs solution at 0.5 g/L for 15 min achieved the highest survival rate. The highest shoot regeneration rate reached 86.66%, with 65.33 shoots/explant from ½-petal explants. Direct formation of the flower consists of two different structural forms, including single petal clusters and complete flower buds, with the corresponding flower formation rates of 19.66 petals/explant; 0.53 flower buds/explant, respectively, at the keel of the petals on the MS medium supplemented with 1000 mg/L myo-inositol, 1.0 mg/L BA, 1.0 mg/L NAA, 30 g/L sucrose and 9.0 g/L agar. Interestingly, increasing the concentration of myo-inositol in the culture medium led to the appearance of red-leaf shoots at the highest rate (12.66 shoots/explant), reaching a maximum shoot height of 1.40 cm after 8 weeks of culture. After each subculture, the red color on leaves tended to decrease gradually, and they were in vitro flowered after the second subculture at a rate of 15% and the third subculture at a rate of 10% on medium supplemented with 40 mg/L adenine, 1.0 mg/L NAA, 1.0 mg/L BA, 30 g/L sucrose, 9 g/L agar and 1 g/L activated charcoal. The plantlets regenerated from petal cultures bloomed with standard red pigments, similar to those grown in natural growing conditions.

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