Effects of simulated microgravity on seed germination, growth, development and accumulated secondary compounds of Hibiscus sagittifolius Kurz. cultured in vitro

Authors

  • Dương Tấn Nhựt Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology
  • Nguyễn Xuân Tuấn Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology
  • Nguyễn Thị Thùy Anh Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology
  • Nguyễn Bá Nam Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology
  • Nguyễn Phúc Huy Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology
  • Hoàng Thanh Tùng Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology
  • Vũ Thị Hiền Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology
  • Vũ Quốc Luận Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology
  • Bùi Thế Vinh Center of Ginseng and Medicinal Materials in Ho Chi Minh City
  • Trần Công Luận Center of Ginseng and Medicinal Materials in Ho Chi Minh City

DOI:

https://doi.org/10.15625/1811-4989/15/1/12322

Keywords:

Clinostat, development, germination, growth, simulated microgravity

Abstract

In the present study, Hibiscus sagittifolius Kurz. seeds were used as the plant materials for studying on the effects of simulated microgravity (on a 2D clinostat) on seed germination, shoot multiplication, growth, development and secondary metabolite accumulation. After surface sterilization, seeds were cultured on MS medium supplemeted with 30 g/l sucrose and 9 g/l agar in Petri dishes (9 seeds per dish, the seed to seed distance of 1.5 cm and kept in the same direction), and maintained in a Clinostat (2 rpm). The results showed that simulated microgravity inhibited the growth and development of Hibiscus sagittifolius roots with root length of 11.83 cm, fresh and dry weight of 58.28 and 5.23 mg, respectively but it made an increase in germination rate (87%) and accumulation of secondary metabolites (the total saponins content of 53.00 mg/g and the total coumarin content of 25.67 mg/g) after 3 weeks of culture. In addition, the simulated microgravity also resulted in positive shoot multiplication (shoot height of 3.07 cm, 6.33 nodes per shoot, 3.33 shoots per explant, and the fresh and dry weight of 401.33 and 37.00 mg, respectively), and growth and development of Hibiscus sagittifolius shoots (plant height of 12.17 cm with 5.67 leaves per shoot together with the average root length of 1.77 cm, and the fresh and dry weight of 419.00 and 36.00 mg) after 4 weeks of culture. The results from this study could be attributed to future perspectives in research on plant breeding and accumulation of secondary metabolites in medicinal plants.

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Published

2018-04-20

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Articles