Improved in vitro rooting and acclimatization of “Violetta” Artichoke and “Green Globe” Artichoke


  • Hoang Dac Khai
  • Nguyen Thi Nhu Mai
  • Hoang Le Lan Anh
  • Nguyen Nhu Minh Nguyet
  • Ho Viet Long
  • Vu Quoc Luan
  • Vu Thi Hien
  • Hoang Thanh Tung Viện Nghiên cứu Khoa học Tây Nguyên
  • Do Manh Cuong
  • Tran Van Lich
  • Tran Thi Nhung
  • Chu Duc Ha
  • Le Van Thuc
  • Duong Tan Nhut



Artichoke, giá thể, ra rễ in vitro, thích nghi vườn ươm, vi nhân giống


Artichoke (Cynara scolymus L.), a medicinal plant with high economic value, contains high levels of phenolic compounds; especially cynarine, which plays an important role in preventing cancer, cardiovascular disease, osteoporosis, diabetes and neurodegeneration, etc. Currently, Artichoke micropropagation has achieved some success; however, the rooting efficiency and plantlet quality are still limited. In this study, improving the quality of Artichoke plantlet related to the shoot quality and suitable substrates in in vitro rooting stage was studied on “Violetta” Artichoke (VA) and “Green Globe” Artichoke (GA). The results showed that shoots (1.5 cm) cultured on MS medium supplemented 0.5 mg/L KIN were most suitable to shoot multiplication of VA with the number of shoots/explant (3.67 shoots), number of shoots ≥ 2 cm (3 shoots); while, 1.0 mg/L BA was suitable to shoot multiplication of GA (5.33 shoots; 5.00 shoots; respectively) after 4 weeks of culture. Besides, the in vitro rooting was improved using 8 g/L commercial agar for VA; meanwwhile, 3 g/L gelrite for GA. In addition, the nylon bag culture system (120 mm × 250 mm) has potential in plantlet production (15 plants/bag) and can be applied for large scale micropropagation. In addition, VA and GA plantlets derived from in vitro culture gave the good acclimatization, growth and development after 8, 12 and 20 weeks cultivating at the green house conditions.


Download data is not yet available.


Acquadro A, Papanice MA, Lanteri S, Bottalico G, Portis E, Campanale A, Gallitelli D (2010) Production and fingerprinting of virus-free clones in a reflowering Globe Artichoke. PCTOC 100(3): 329-337.

Barpete S, Khawar KM, Özcan S (2014) Differential competence for in vitro adventitious rooting of Grass pea (Lathyrus sativus L). PCTOC 119(1): 39-50.

Bedini L, Lucchesini M, Bertozzi F, Graifenberg A (2012) Plant tissue cultures from four Tuscan Globe Artichoke cultivars. Open Life Sci 7(4): 680-689.

Bianco P, Robey PG (2000) Marrow stromal stem cells. J Clin Invest 105(12): 1663-1668.

Bigot C, Foury C, Casenave M, Delacroix E, Delage C, Godin B, Lebœuf J (1984) Multiplication in vitro d'Artichaut (Cynara scolymus L.) à partir de semences: comparaison au champ de quelques clones à la lignée dont ils sont issus. Agronomic 4(8): 669-710.

Brutti C, Apostolo NM, Ferrarotti SA, Llorente BE, Krymkiewicz N (2000) Micropropagation of Cynara scolymus L. employing cyclodextrins to promote rhizogenesis. Sci Hort 83(1): 1-10.

Catacora E, Olivera J, Ramos Z, Alve Z, Pinedo R (2019) Micropropagation of clonal lines of thorny Artichoke (Cynara scolymus L.). PJA 3(1): 29-38.

Clifford MN, Brown JE (2006) Dietary Flavonoids and Health - Broadening the Perspective. In: Andersen ØM, Markham KR (Eds). Flavonoid: Chemistry, Biochemistry and Applications. CRC Press, pp. 319-370.

Comino C, Moglia A, Repetto A, Tavazza R (2019) Globe artichoke tissue culture and its biotechnological application. In: Portis E, Acquadro A, Lanteri S (Eds). The Globe Artichoke Genome. Springer, pp. 41-64.

de Falco B, Incerti, G, Amato, M, Lanzotti, V (2015) Artichoke: botanical, agronomical, phytochemical, and pharmacological overview. Phytochem Rev 14(6): 993-1018.

de Leo P, Greco B (1976) A new technique for propagation of Artichoke: in vitro culture of apical meristems. Proceeding II Internation Congress Artichoke. Medica, Italy, 657-665.

Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11(1): 1-42.

El-Boullani R, Elmoslih A, El-Finti A, El-Mousadik A, Serghini MA (2013) Effect of decapitation and size of explants on in vitro multiplication rate of globe artichoke. Acta Hort (983): 325-329.

El-Zeiny OAH, El-Behairy UA, Zocchi G, Rashwan MM (2013) Commercial production of Globe Artichoke (Cynara scolymus L.) in vitro. EJAR 91(3): 933-1007.

Ercan N (2016) Effects of various growth regulators on in vitro rooting of Globe Artichoke (Cynara scolymus L.). J Agri Sci Technol A 6, 335-340.

Iapichino, G (1996) Micropropagation of globe artichoke (Cynara scolymus L.) from underground dormant buds (“ovoli”). In Vitro Cell Dev Biol Plant 32(4): 249-252.

Labrousse, P, Delmail, D, Decou, R, Carlué, M, Lhernould, S, Krausz, P (2012) Nemesia root hair response to paper pulp substrate for micropropagation. Sci World J 2012: 1-7.

López-Pérez AJ, Martínez JA (2015) In vitro root induction improvement by culture in darkness for different Globe Artichoke cultivars. In Vitro Cell Dev Biol 51(2): 160-165.

Marras F, Foddai A, Corda P (1985) La difesa del carciofo dale malattie crittogamiche. Inf Fitopatol 35(8):19-24.

Minutillo SA, Mascia T, Gallitelli D (2012) A DNA probe mix for the multiplex detection of ten Artichoke viruses. Eur J Plant Pathol 134(3): 459-465.

Morzadec JM, Hourmant A (1997) In vitro rooting improvement of Globe Artichoke (cv. Camus de Bretagne) by GA3. Sci Hort 72(1): 59-62.

Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with Tobacco tissue cultures. Physiol Plant 15(3): 473-497.

Ozsan T, Onus AN (2019) A protocol on in vitro rooting of ‘Bayrampaşa’ Artichoke (Cynara scolymus L.). Mediterr Agric Sci 32(2): 129-134.

Pandino G, Lombardo, S Mauromicale, G Williamson, G (2011) Phenolic acids and flavonoids in leaf and floral stem of cultivated and wild Cynara cardunculus L. genotypes. Food Chem 126(2): 417-422.