Assessment of fungi and viruses in Artichoke (Cynara scolymus L.) in Da Lat, Lam Dong province

Authors

  • Hoang Thanh Tung Viện Nghiên cứu Khoa học Tây Nguyên https://orcid.org/0000-0002-2614-0429
  • Hoang Dac Khai
  • Do Manh Cuong
  • Le Van Thuc
  • Le The Bien
  • Ho Viet Long
  • Vo Ha Tuyet Hanh
  • Hoang Le Lan Anh
  • Nguyen Thi Nhu Mai
  • Nguyen Nhu Minh Nguyet
  • Vu Thi Hien
  • Vu Quoc Luan
  • Nguyen Khoa Truong
  • Le Ngoc Trieu
  • Hoang Thi Nhu Phuong
  • Duong Tan Nhut

DOI:

https://doi.org/10.15625/1811-4989/18/4/15130

Keywords:

Artichoke, disease-free, fungi, Tomato mosaic virus

Abstract

Artichoke (Cynara scolymus L.), a high economic value crop, was brought to Vietnam by the French in the late 19th century. The artichoke was mainly planted in Lam Dong, Lao Cai, Vinh Phuc provinces, etc. At present, the disease situation of Artichoke plants and the lack of disease-free seedlings result in insufficient source of Artichoke for producers. Artichoke plants are mainly vegetative propagation and pathogens easily transferred from mother to daughter plants. Therefore, low propagation rate and fungal infection are two main factors hindering the expansion and development of Artichoke cultivation (in Lam Dong). Therefore, studying and evaluating the situation of fungi and viruses as well as establishing the in vitro propagation procedures in order to produce high number of disease-free seedlings are urgent for the current Artichoke shortage. In this study, samples of purple and white Artichoke varieties, which suspected fungal manifestations, were collected to assessment of fungi and viruses in artichoke. In addition, in vitro propagation by cultivating apical meristem was applied to produce disease-free seedlings. The recorded results showed that, 19 strains of mold were identified on purple and white Artichoke belonged to nine genera including Mucor (M. sp., M. circinelloides, M. fragilis, M. irregularis, and M. racemosus), Alternaria (Alternaria sp., A. alterinata, A. gaisen, A. tenuissima, and A. tillandsiae), Fusarium (F. acuminatum and F. solani), Cylindrobasidium (Cylindrobasidium sp1 and Cylindrobasidium sp2), Actinomucor elegans, Curvalaria clavata, Plectosphaerella oligotrophica, Phoma herbarum, Rhizomucor variabillis; meanwhile, the Tomato mosaic virus (ToMV) was isolated only purple Artichoke. Shoot explants obtained from apical meristem culture were completely disease-free and used for micropropagation at the next stage.

Downloads

Download data is not yet available.

References

Bariana HS (2016) Detection of five seedborne legume viruses in one sensitive multiplex polymerase chain reaction test. American Phytopathl Soc 84(10): 1201-1205.

Bundy R, Walker AF, Middleton RW (2004) Artichoke leaf extract reduces symptoms of irritable bowel syndrome and improves quality of life in otherwise healthy volunteers suffering from concomitant dyspepsia: a subset analysis. J Altern Complement Med 10(4): 667-669.

Gallitelli D, Mascia T, Martelli GP (2012) Viruses in artichoke. Adv Virus Res 84: 289-324.

Kumar S, Khan M S, Raj SK, Sharma AK (2009) Elimination of mixed infection of Cucumber mosaic and Tomato aspermy virus from Chrysanthemum morifolium Ramat. cv. Pooja by shoot meristem culture. Sci Hort 119: 108-112.

Kyriakopoulou PE (1995) Artichoke Italian latent virus causes artichoke patchy chlorotic stunting disease. Ann Appl Biol 127: 489-497.

Lam Dong Agricultural Center (2017) Artichoke planting technical process. http://khuyennong.lamdong.gov.vn/du-lieu-khuyen-nong/ky-thuat-trong-trot/1685-quy-tr%C3%ACnh-k%E1%BB%B9-thu%E1%BA%ADt-tr%E1%BB%93ng-c%C3%A2y-atis%C3%B4

Ministry of Health (2009) Viet Nam Pharmacopoeia IV, Hanoi.

Minutillo SA, Mascia T, Gallitelli D (2012) A DNA probe mix for the multiplex detection of ten artichoke viruses. European J Plant Pathol 134(3): 459–465.

Murashuige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobaco tissue culture. Physiol Plant 15(3): 473-479.

Sanchez F, Wang X, Jenner CE, Walsh JA, Ponz F (2003) Strains of Turnip mosaic poty virus as defined by the molecular analysis of the coat protein gene of the virus. Virus Res 94: 33-43

Silva RM, Souto ER, Pedroso JC, Arakava R, Almeida AMR, Barboza, AAL, Vida JB (2008) Detection and identification of TMV infecting tomato under protected cultivation in Paraná State. Brazilian Arch Biol Tech 51(5): 903-909.

Sivparas BJ, Gubba A (2008) Isolation and molecular characterization of

Tomato spotted wilt virus (TSWV) isolates occuring in South Africa. African

J Agricultl Res 3: 428-434.

Proctor AG (1977) Mycological method. In: Collins CH, Lyne PM (Eds) Microbiological methods, 4th editions. Butter Worths, UK.

Wang M, Simon JE, Aviles IF, He K, Zheng QY, Tadmor Y (2003) Analysis of antioxidative phenolic compounds in artichoke (Cynara scolymus L.). J Agric Food Chem 51(3): 601-608.

Weising K, Nybom H, Wolff K, Kahl G (2005) DNA Fingerprinting in Plants

Principles, Methods, and applications (second Edition). Cpc Press Taylor and Fancies group.

Wittemer SM, Ploch M, Windeck T, Müller SC, Drewelow B, Derendorf H, Veit M (2005) Bioavailability and pharmacokinetics of caffeoylquinic acids and flavonoids after oral administration of Artichoke leaf extracts in humans. Phytomedicine 12(1-2): 28-38.

Downloads

Published

2021-05-24

Issue

Section

Articles