Haemagglutination activity of lectins from extracts of some Vietnam marine sponges
Author affiliations
DOI:
https://doi.org/10.15625/1811-4989/17/4/14457Keywords:
Carbohydrate-binding specificity, haemagglutination activity, lectins, marine sponges, stabilityAbstract
Aqueous extracts from 16 species of Vietnam marine sponges were examined for haemagglutination activity using native and enzyme-treated different animal and human erythrocytes. Among these, extracts from 14 species were found to have haemagglutinination activities toward at least one type of erythrocyte tested meaning that 87.5% of the surveyed marine sponge species possess haemagglutination activity. Strong activity was detected in extracts from marine sponge species Acanthella cavernosa, Axinyssa sp., Cinachyrella sp., 01NT.2.4, 3.5, 3.10, 3.11 and 3.18 with enzyme-treated various animal and human erythrocytes. In a haemagglutination–inhibition test with various monosaccharides and glycoproteins, haemagglutination activity of the extract from A. cavernosa had no affinity for any of the monosaccharides, but inhibited by porcine stomach mucin and fetuin, whereas activities of the extract from Cinachyrella sp. were strongly inhibited by monosaccharides, such as D-galactose and N-acetyl-D-galatosamine, but not with glycoproteins. The activity of Stylissa flexibilis extract was inhibited by D-galactose, porcine stomach mucin, fetuin and their asialo derivatives, suggesting the presence of lectin specific for O-glycans of this species. The activities of four sponge extracts from A. cavernosa, S. flexibilis, Axinyssa sp. and Cinachyrella sp. were stable over a wide range of pH and temperature. Haemagglutination activities of A. cavernosa, Axinyssa sp. and Cinachyrella sp. extracts were independent of the presence of divalent cations, except for the haemagglutination activity of extract from S. flexibilis, which was dependent on the presence of divalent cations. The results suggest that Vietnam marine sponges may be good sources of useful lectins for biochemical and biomedical applications.
Downloads
References
Atta AM, Barral-Netto M, Peixinho S, Sousa-Atta ML (1989) Isolation and functional
characterization of a mitogenic lectin from the marine sponge Cinachyrella alloclada. Braz J Med Biol Res 22: 379–385.
Atta AM, Menezes EP, Peixinho S, Sousa-Atta MLB (1990) Isolation of a lectin from the marine sponge Desmapsama anchorata by affinity chromatography on raffinose-Sepharose 6B. Braz J Med Biol Res 23: 191–194.
Bakus GJ, Green G (1974) Toxicity in sponges and holothurians: A geographic pattern. Science 185: 951–953.
Berlinck RGS, Hajdu E, Rocha RM, Oliveira JHHL, Hernandez ILC, Seleghim MHR, Granato AC, Almeida EVR, Nunez CV, Muricy G (2004) Challenges and rewards of research in marine natural products chemistry in Brazil. J Nat Prod 67: 510–522.
Bretting H, Kabat EA (1976) Purification and characterization of the agglutinins from the sponge Axinella polypoides and a study of their combining sites. Biochemistry 15: 3228–3236.
Bretting H, Phillips SG, Klumpart HJ, Kabat EAA (1981a) Mitogenic lactose binding lectin from the sponge Geodia cydonium. J Immunol 127: 1652–1658.
Bretting H, Donadey C, Vacele J, Jacobs G (1981b) Investigation on the occurrence of lectins in marine sponges with special regard to some species ofthe family Axinellidae. Comp Biochem Physiol B 70: 69–76.
Cao Đăng Nguyên (1998) Điều tra lectin từ các mẫu sinh vật thường gặp ở vùng biển Hải Hậu. Tạp chí Sinh học 20: 122-127.
Cao Đăng Nguyên, Nguyễn Quốc Khang (1998) Tinh sạch, hoạt tính sinh học và khả năng phản ứng tế bào của lectin trai tai tượng (Tridacna elongata). Tạp chí Di truyền học và Ứng dụng 2: 32-35.
Carneiro RF, de Melo AA, Nascimento FEPD, Simplicio CA, Nascimento KS, Rocha BAMD, Saker-Sampaio S, Moura RDM, Mota SS, Cavada BS (2013) Halilectin 1 (H-1) and Halilectin 2 (H-2): Two new lectins isolated from the marine sponge Haliclona caerulea. J Mol Recognit 26: 51–58.
Carneiro RF, de Melo AA, de Almeida AS, Moura RM, Chaves RP, de Sousa R, Nascimento KS, Sampaio SS, Lima JP, Cavada BS (2013b) H-3, a new lectin from the marine sponge Haliclona caerulea: Purification and mass spectrometric characterization. Int J Biochem Cell Biol 45: 2864–2873.
Carte BK (1996) Biomedical potential of marine natural products. Bioscience 46: 271–286.
Dinh Thanh Trung, Vo Thi Dieu Trang, Ngo Thi Duy Ngoc, Phan Thi Hoai Trinh, Tran Thi Hai Yen, Le Dinh Hung (2017) Haemagglutination activity of the extracts from some Vietnam marine invertebrates. Vietnam J Biotechnol 15: 691-701.
Dodd RY, MacLennan AP, Hawkins DC (1968) Haemagglutinins from marine sponges. Vox Sanguinis 15: 386–391.
Dresch RR, Zanetti GD, Lerner CB, Mothes B, Trindade VMT, Henriques AT, Vozári-Hampe MM (2008) ACL-I, a lectin from the marine sponge Axinella corrugata: Isolation, characterization and chemotactic activity. Comp Biochem Physiol Toxicol Pharmacol 148: 23–30.
Dresch RR, Lerner CB, Mothes B, Trindade VMT, Henriques AT, Vozári-Hampe MM (2012) Biological activities of ACL-I and physicochemical properties of ACL-II, lectins isolated from the marine sponge Axinella corrugata. Comp Biochem Physiol Biochem Mol Biol 161: 365–370.
Dresch RR, Zanetti GD, Irazoqui FJ, Sendra VG, Zlocowski N, Bernardi A, Rosa RM, Battastini AMO, Henriques AT, Vozári-Hampe MM (2013) Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata. Biotech Histochem 88: 1–9.
Engel M, Bachmann M, Schröder HC, Rinkevich B, Kljajic Z, Uhlenbruck G,
Müller WEG (1992) A novel galactose- and arabinose-specific lectin from the sponge Pellina semitubulosa: Isolation, characterization and immunobiological properties. Biochimie 74: 527–537.
Gardères J, Bourguet-Kondracki ML, Hamer B, Batel R, Schröder HC, Mülle WEG (2015) Porifera Lectins: Diversity, Physiological Roles and Biotechnological Potential. Mar Drugs 13: 5059-5101.
Gundacker D, Leys SP, Schröder HC, Müller IM, Müller WEG (2001) Isolation and cloning of a C-type lectin from the hexactinellid sponge Aphrocallistes vastus: A putative aggregation factor. Glycobiology 11: 21–29.
Kawsar SMA, Fujii Y, Matsumoto R, Ichikawa T, Tateno H, Hirabayashi J, Yasumitsu H, Dogasaki C, Hosono M, Nitta K, Hamako J, Matsui T, Ozeki Y (2008) Isolation, purification, characterization and glycan-binding profile of a D-galactoside specific lectin from the marine sponge, Halichondria okadai. Comp Biochem Physiol B 150: 349–357.
Kawsar SMA, Mamun SMA, Rahman MDS, Hidetaro Y, Ozeki Y (2011) In vitro antibacterial and antifungal effects of a 30 kDa D-galactoside-specific lectin from the desmosponge Helichondria okadai. Int J Biol Life Sci 7: 31–37.
Kurata O, Hatai K (2002) Activation of carp leukocytes by a galactose binding protein from Aphanomyces piscicida. Dev Comp Immunol 26: 461–469.
Le Dinh Hung, Hori K, Huynh Quang Nang (2009a) Screening and preliminary characterization of hemagglutinins in Vietnamese marine algae. J Appl Phycol 21: 89–97.
Le Dinh Hung, Sato T, Shibata H, Hori K (2009b) Biochemical comparison of lectins among three different color strains of the red alga Kappaphycus alvarezii. Fish Sci 75: 723–730.
Le Dinh Hung, Hori K, Huynh Quang Nang, Tran Kha, Le Thi Hoa (2009c) Seasonal changes in growth rate, carrageenan yield and lectin content in the red alga Kappaphycus alvarezii cultivated in Camranh Bay, Vietnam. J Appl Phycol 21: 265-272.
Le Dinh Hung LD, Sato Y, Hori K (2011) High-mannose N-glycan specific lectins from the red alga Kappaphycus striatum (Carrageenophyte). Phytochemistry 72: 855-861.
Le Dinh Hung, Bui Minh Ly, Vo Thi Dieu Trang, Ngo Thi Duy Ngoc, Le Thi Hoa, Phan Thi Hoai Trinh (2012) A new screening for hemagglutinins from Vietnamese marine macroalgae. J Appl Phycol 24: 227-235.
Le Dinh Hung, Ngo Thi Duy Ngoc, Kanji Hori (2013) Isolation and characterization of novel lectin from the red alga Gracilaria salicornia. Vietnam J Biotechnol 11: 743-753.
Le Dinh Hung, Hirayama M, Bui Min Ly, Hori K (2015a) Purification, primary structure and biological activity of high-mannose N-glycan specific lectin from the cultivated Eucheuma denticulatum. J Appl Phycol 27: 1657-1669.
Le Dinh Hung, Hirayama M, Bui Minh Ly, Hori K (2015b) Biological activity, cDNA cloning and primary structure of lectin KSA-2 from the cultivated red alga Kappaphycus striatum (Schmitz) Doty ex Silva. Phytochem Lett 14: 99–105.
Le Dinh Hung, Makoto Hirayama, Kanji Hori (2016) Cloning and characterizing cDNA sequences coding high-mannose N-glycan binding lectins from cultivated red algae Eucheuma denticulatum and Kappaphycus striatum. Vietnam J Biotechnol 14: 327-336.
Le Dinh Hung, Tran Thi Hai Yen, Dinh Thanh Trung (2018a) Characterization of O-glycan binding lectin from the red alga Hydropuntia eucheumatoides. Vietnam J Biotechnol 16: 687-696.
Le Dinh Hung, Bui Minh Ly, Vo Thi Hao, Dinh Thanh Trung, Vo Thi Dieu Trang, Phan Thi Hoai Trinh, Ngo Thi Duy Ngoc, Thai Minh Quang (2018b) Purification, characterization and biological effect of lectin from the marine sponge Stylissa flexibilis (Lévi, 1961). Comp Biochem Physiol Part B 216: 32-38.
Le Dinh Hung, Le Thi Hoa, Le Nhu Hau, Dinh Thanh Trung (2019) The lectin accumulation, growth rate, carrageenan yield, and quality from the red alga Kappaphycus striatus cultivated at Camranh Bay, Vietnam. J Appl Phycol 31: 1991–1998.
Mebs D, Weiler I, Heinke HF (1985) Bioactive proteins from marine sponges: Screening of sponge extracts for hemagglutinating, hemolytic, ichthyotoxic and lethal properties and isolation and characterization of hemagglutinins. Toxicon 23: 955–962.
Medeiros DS, Medeiros TL, Ribeiro JKC, Monteiro NKV, Migliolo L, Uchoa AF, Vasconcelos IM Oliveira AS, de Sales MP, Santos EA (2010) A lactose specific lectin from the sponge Cinachyrella apion: Purification, characterization, N-terminal sequences alignment and agglutinating activity on Leishmania promastigotes. Comp Biochem Physiol Biochem Mol Biol 155: 211–216.
Miarons PB, Fresno M (2000) Lectins from tropical sponges. Purification and characterization of lectins from genus Aplysina. J Biol Chem 275: 29283–29289.
Mistry AC, Honda S, Hirose S (2001) Structure, properties and enhanced expression of galactose-binding C-type lectins in mucous cells of gills from freshwater Japanese eels (Anguilla japonica). Biochem J 360: 107–115.
Moura RM, Queiroz AFS, Fook JMSLL, Dias ASF, Monteiro NKV, Ribeiro JKC, Moura GEDD, Macedo LLP, Santos EA, Sales MP (2006) CvL, a lectin from the marine sponge Cliona varians: Isolation, characterization and its effects on pathogenic bacteria and Leishmania promastigotes. Comp Biochem Physiol Mol Integr Physiol 145: 517–523.
Nguyễn Quốc Khang, Trần Quốc Vượng, Cao Đăng Nguyên (2000) Một vài tính chất lý, hoá học của lectin cá nheo (Prasilurus asotus). Tạp chí sinh học 22: 149-152.
Pajic I, Kljajic Z, Dogovic N, Sladic D, Juranic Z, Gasic MJ (2002) A novel lectin from the sponge Haliclona cratera: Isolation, characterization and biological activity. Comp Biochem Physiol Toxicol Pharmacol 132: 213–221.
Queiroz AFS, Moura RM, Ribeiro JKC, Lyra IL, Cunha DCS, Santos EA, De-Sales MP (2008) Pro-inflammatory effect in mice of CvL, a lectin from the marine sponge Cliona varians. Comp Biochem Physiol C 147: 216–221.
Queiroz A, Silva R, Moura R, Dreyfuss J, Paredes-Gamero E, Souza A, Tersariol I, Santos E, Nader H, Justo G (2009) Growth inhibitory activity of a novel lectin from Cliona varians against K562 human erythroleukemia cells. Cancer Chemother Pharmacol 63: 1023–1033.
Schröder HC, Kljajic Z, Weiler BE, Gasic M, Uhlenbruck G, Kurelec B, Müller WEG (1990) The galactose-specific lectin from the sponge Chondrilla nucula displays anti-human immunodeficiency virus activity in vitro via stimulation of the (2′-5′) oligoadenylate metabolism. Antivir Chem Chemother 1: 99–105.
Schröder HC, Ushijima H, Krasko A, Gamulin V, Thakur NL, Diehl-Seifert B, Müller IM, Müller WEG (2003) Emergence and disappearance of an immune molecule, an antimicrobial lectin, in basal metazoa. A tachylectin-related protein in the sponge Suberites domuncula. J Biol Chem 278: 32810–32817.
Schröder HC, Boreiko A, Korzhev M, Tahir MN, Tremel W, Eckert C, Ushijima H, Müller IM, Müller WEG (2006) Co-expression and functional interaction of silicatein with galectin: Matrix-guided formation of siliceous spicules in the marine demosponge Suberites domuncula. J Biol Chem 281: 12001–12009.
Sepcic K, Kauferstein S, Mebs D, Turk T (2010) Biological activities of aqueous and organic extracts from tropical marine sponges. Mar Drugs 8: 1550–1566.
Sharon N, Lis H (2003) Lectins, second ed. Kluwer Academic Publishers, The Netherlands.
Vo TS, Kim SK (2010) Potential anti-HIV agents from marine resources: an overview. Marine Drugs 8: 2871–2892.
Xiong C, Li W, Liu H, Zhang W, Dou J, Bai X, Du Y, Ma X (2006) A normal mucin-binding lectin from the sponge Craniella australiensis. Comp Biochem Physiol Toxicol Pharmacol 143: 9–16.
Yousif AN, Albright LJ, Evelyn TPT (1994) Purification and characterization of a galactose-specific lectin from eggs of coho salmon Oncorhynchus kisutch and its interaction with bacterial fish pathogens. Dis Aquat Org 20: 127–136.
Downloads
Published
How to Cite
Issue
Section
