Impact of metal contamination to the emergence of (multi) antibiotic resistant bacteria in Thai Nguyen, Vietnam

Authors

  • Pham Hoang Nam University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Quang Huy University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Le Thi Thu Hang University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Thy Ngoc University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Thi Kieu Oanh University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Bui Van Hoi University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Ngoc Lien University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Thi Thu Hien Hanoi Open University, B101 Nguyen Hien Street, Ha Ba Trung District, Hanoi, Vietnam
  • Le Mai Huong Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Dinh Luyen Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Thi Huyen Thu Department of Pharmacy, Saint Paul Hospital, 12 Chu Van An Street, Ba Dinh District, Hanoi, Vietnam
  • Tran Thi Hong Minh Hanoi University of Natural Resources and Environment, 41A Phu Dien Road, North-Tu Liem District, Hanoi, Vietnam
  • Nguyen Tien Dat Center for Research and Technology Transfer, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Marie-Genevieve Dijoux-Franca UMR 5557, Ecologie Microbienne, CNRS, INRA, VetagroSup, UCBL, Université de Lyon, Villeurbanne, France

DOI:

https://doi.org/10.15625/1811-4989/13509

Keywords:

antibiotic, emergence, metal contamination, multidrug resistant, Pb

Abstract

Heavy metal contamination in the environment due to anthropogenic activities in industrial and agricultural areas represents stress for the living ecosystem including soil microorganisms. The adaptive response of bacterial communities to these pollutants can be observed through the increasing proportion of resistant bacteria. Our study aimed at evaluating the effect of metal contamination to the emergence of bacteria resistant to 3 common antibiotics, ie., gentamycin, sulfamethoxazole, and norfloxacin in Tan Long Pb-Zn mining site, Thai Nguyen province by a culture-dependent approach. The results showed that under moderated Pb contamination, a significant increase in prevalence of gentamycin-resistant bacteria was observed. Antibiotic susceptibility test showed 45% of isolates resistant to at least two antibiotics among gentamycin, norfloxacin, tetracyclin, imipenem, andsulfamethoxazole, and 10.4 % of isolates resistant tho three antibiotics. More detailed investigations in molecular analysis are needed to identify and genetically characterize of multi-drug resistant isolates as well as to better understand the link between metal resistance and (multi)resistance to antibiotics.

Downloads

Download data is not yet available.

References

Al-Odaini, N. A., Zakaria, M. P., Yaziz, M. I., & Surif, S. (2010). Multi-residue analytical method for human pharmaceuticals and synthetic hormones in river water and sewage effluents by solid-phase extraction and liquid chromatography-tandem mass spectrometry. J. Chromatogr. A, 1217(44), 6791-6806.

Aendekerk, S., Ghysels, B., Cornelis, P., Baysse, C., 2002. Characterization of a new efflux pump, MexGHI-OpmD, from Pseudomonas aeruginosa that confers resistance to vanadium. Microbiol. Read. Engl. 148, 2371–2381. https://doi.org/10.1099/00221287-148-8-2371

Alvarez-Ortega, C., Olivares, J., Martínez, J.L., 2013. RND multidrug efflux pumps: what are they good for? Front. Microbiol. 4. https://doi.org/10.3389/fmicb.2013.00007

Anh, B.T.K., Kim, D.D., Tua, T.V., Kien, N.T., Anh, D.T., 2011. Phytoremediation potential of indigenous plants from Thai Nguyen province, Vietnam. J. Environ. Biol. Acad. Environ. Biol. India 32, 257–262.

Berg, J., Thorsen, M.K., Holm, P.E., Jensen, J., Nybroe, O., Brandt, K.K., 2010. Cu Exposure under Field Conditions Coselects for Antibiotic Resistance as Determined by a Novel Cultivation-Independent Bacterial Community Tolerance Assay. Environ. Sci. Technol. 44, 8724–8728. https://doi.org/10.1021/es101798r

Bui, T.K.A., Dang, D.K., Nguyen, T.K., Nguyen, N.M., Nguyen, Q.T., Nguyen, H.C., 2014. Phytoremediation of heavy metal polluted soil and water in Vietnam. J. Vietnam. Environ. 6, 47–51. https://doi.org/10.13141/jve.vol6.no1.pp47-51.g81

Dang, D.K., 2010. Study on phytoremediation of heavy metal - contaminated soils in mining areas. National project KC08.04/06-10.

Dang, T.A., Chu, T.T.H., 2005. The influence of metals in soil and exposure time to the accumulation of metals in some vegetables. The problems of basic research in life sciences 361–364.

Deredjian, A., Alliot, N., Blanchard, L., Brothier, E., Anane, M., Cambier, P., Jolivet, C., Khelil, M.N., Nazaret, S., Saby, N., Thioulouse, J., Favre-Bonté, S., 2016. Occurrence of Stenotrophomonas maltophilia in agricultural soils and antibiotic resistance properties. Res. Microbiol.

https://doi.org/10.1016/j.resmic.2016.01.001

Deredjian, A., Colinon, C., Brothier, E., Favre-Bonté, S., Cournoyer, B., Nazaret, S., 2011. Antibiotic and metal resistance among hospital and outdoor strains of Pseudomonas aeruginosa. Res. Microbiol. 162, 689–700. https://doi.org/10.1016/j.resmic.2011.06.007

E1*, B., C1, V., E2, S., Y3, K., A1, A., S1, P., Varzakas T4, 2016. Emerging Antibiotic Resistance in Honey as a Hazard for Human Health. J. Bacteriol. Mycol. Open Access 2, 1–6. https://doi.org/10.15406/jbmoa.2016.2.00012

Ghosh, S., LaPara, T.M., 2007. The effects of subtherapeutic antibiotic use in farm animals on the proliferation and persistence of antibiotic resistance among soil bacteria. ISME J. 1, 191–203. https://doi.org/10.1038/ismej.2007.31

Hoa, P.T.P., Managaki, S., Nakada, N., Takada, H., Shimizu, A., Anh, D.H., Viet, P.H., Suzuki, S., 2011. Antibiotic contamination and occurrence of antibiotic-resistant bacteria in aquatic environments of northern Vietnam. Sci. Total Environ. 409, 2894–2901. https://doi.org/10.1016/j.scitotenv.2011.04.030

Knapp, C.W., McCluskey, S.M., Singh, B.K., Campbell, C.D., Hudson, G., Graham, D.W., 2011. Antibiotic Resistance Gene Abundances Correlate with Metal and Geochemical Conditions in Archived Scottish Soils. PLOS ONE 6, e27300. https://doi.org/10.1371/journal.pone.0027300

Liebert, C.A., Hall, R.M., Summers, A.O., 1999. Transposon Tn21, Flagship of the Floating Genome. Microbiol. Mol. Biol. Rev. 63, 507–522.

Lien, L.T.Q., Lan, P.T., Chuc, N.T.K., Hoa, N.Q., Nhung, P.H., Thoa, N.T.M., Diwan, V., Tamhankar, A.J., Stålsby Lundborg, C., 2017. Antibiotic Resistance and Antibiotic Resistance Genes in Escherichia coli Isolates from Hospital Wastewater in Vietnam. Int. J. Environ. Res. Public. Health 14. https://doi.org/10.3390/ijerph14070699

Marshall, B.M., Levy, S.B., 2011. Food Animals and Antimicrobials: Impacts on Human Health. Clin. Microbiol. Rev. 24, 718–733. https://doi.org/10.1128/CMR.00002-11

Miller, D.D., Rutzke, M.A., 2010. Atomic Absorption Spectroscopy, Atomic Emission Spectroscopy, and Inductively Coupled Plasma-Mass Spectrometry, in: Food Analysis, Food Analysis. Springer, Boston, MA, pp. 421–442. https://doi.org/10.1007/978-1-4419-1478-1_24

Mirzaei, R., Yunesian, M., Nasseri, S., Gholami, M., Jalilzadeh, E., Shoeibi, S., Bidshahi, H.S., Mesdaghinia, A., 2017. An optimized SPE-LC-MS/MS method for antibiotics residue analysis in ground, surface and treated water samples by response surface methodology- central composite design. J. Environ. Health Sci. Eng. 15. https://doi.org/10.1186/s40201-017-0282-2

Moghannem, S.A., Refaat, B.M., El-Sherbiny, G.M., El-Sayed, M.H., Elsehemy, I.A., Kalaba, M.H., 2015. Characterization of heavy metal and antibiotic-resistant bacteria isolated from polluted localities in Egypt. Egypt. Pharm. J. 14, 158. https://doi.org/10.4103/1687-4315.172856

Perron, K., Caille, O., Rossier, C., Delden, C. van, Dumas, J.-L., Köhler, T., 2004. CzcR-CzcS, a Two-component System Involved in Heavy Metal and Carbapenem Resistance in Pseudomonas aeruginosa. J. Biol. Chem. 279, 8761–8768. https://doi.org/10.1074/jbc.M312080200

Pham, H.N., Michalet, S., Bodillis, J., Nguyen, T.D., Nguyen, T.K.O., Le, T.P.Q., Haddad, M., Nazaret, S., Dijoux-Franca, M.-G., 2017. Impact of metal stress on the production of secondary metabolites in Pteris vittata L. and associated rhizosphere bacterial communities. Environ. Sci. Pollut. Res. Int. 24, 16735–16750. https://doi.org/10.1007/s11356-017-9167-2

Phuong Hoa, P.T., Nonaka, L., Hung Viet, P., Suzuki, S., 2008. Detection of the sul1, sul2, and sul3 genes in sulfonamide-resistant bacteria from wastewater and shrimp ponds of north Vietnam. Sci. Total Environ. 405, 377–384. https://doi.org/10.1016/j.scitotenv.2008.06.023

Youenou, B., Favre-Bonté, S., Bodilis, J., Brothier, E., Dubost, A., Muller, D., Nazaret, S., 2015. Comparative Genomics of Environmental and Clinical Stenotrophomonas maltophilia Strains with Different Antibiotic Resistance Profiles. Genome Biol. Evol. 7, 2484–2505. https://doi.org/10.1093/gbe/evv161

Zhou, Y., Xu, Y.-B., Xu, J.-X., Zhang, X.-H., Xu, S.-H., Du, Q.-P., 2015. Combined Toxic Effects of Heavy Metals and Antibiotics on a Pseudomonas fluorescens Strain ZY2 Isolated from Swine Wastewater. Int. J. Mol. Sci. 16, 2839–2850. https://doi.org/10.3390/ijms16022839

Zhu, Y.-G., Johnson, T.A., Su, J.-Q., Qiao, M., Guo, G.-X., Stedtfeld, R.D., Hashsham, S.A., Tiedje, J.M., 2013. Diverse and abundant antibiotic resistance genes in Chinese swine farms. Proc. Natl. Acad. Sci. U. S. A. 110, 3435–3440. https://doi.org/10.1073/pnas.1222743110

Downloads

Published

2022-03-30

How to Cite

Hoang Nam, P., Quang Huy, N., Thi Thu Hang, L., Thy Ngoc, N., Thi Kieu Oanh, N., Van Hoi, B., Ngoc Lien, N., Thi Thu Hien, N., Mai Huong, L., Dinh Luyen, N., Thi Huyen Thu, N., Thi Hong Minh, T., Tien Dat, N., & Dijoux-Franca, M.-G. (2022). Impact of metal contamination to the emergence of (multi) antibiotic resistant bacteria in Thai Nguyen, Vietnam. Vietnam Journal of Biotechnology, 20(1), 187–196. https://doi.org/10.15625/1811-4989/13509

Issue

Section

Articles