Investigating the diversity of arbuscular mycorrhizal fungi from Gymnema sylvestre and Curcuma longa in Vietnam
Keywords:Arbuscular mycorrhizal, AM fungal diversity, Gymnema sylvestre, Curcuma longa
Arbuscular mycorrhizal (AM) fungi are soil eukaryotes that belong to phylum Glomeromycota and have symbiosis with the vast majority of higher plants’ roots. AM fungi are believed to be coevolved with terrestrial plants, the abundance and diversity of AM fungal communities as a result are host plant dependent. A survey of AM fungi from the rhizospheres of medicinal plants in Northern Vietnam including gurma Gymnema sylvestre and turmeric Curcuma longa was carried out. From the extracted total DNAs of the medicinal plants’ rhizosphere soil samples, 35 mycorrhizal fungal species were identified by analyzing small subunit rRNA gene sequences. Result revealed that genus Glomus is the most abundant in the AM communities of G. sylvestre and C. longa, followed by Gigaspora and Acaulospora. Besides, AM species belonging to genera Scutellospora, Diversispora and Rhizophagus were observed in almost all rhizosphere soil samples. The spore counting by wet sieving and decanting method uncovered a variation in AM spore density of gurma and turmeric rhizosphere. In general, AM species were found more abundantly and more diverse in collected rhizome soil samples of C. longa (27 species belonging to 10 genera) than of G. sylvestre (17 species found belonging to 7 genera). The observed difference in AM communities of G. sylvestre and C. longa supports evidence for the dependence of AM fungal species on host plants, and indicates that AM fungi may have relation to the host plants’ secondary metabolite production.
Badri DV, Vivanco JM (2009) Regulation and function of root exudates. Plant Cell Environ 32(6): 666-681.
Furlan V, Bartschi H, Fortin JA (1980) Media for density gradient extraction of endomycorrhizal spores. Trans Br Mycol Soc 75(2): 336-338.
Gerdemann JW, Nicolson TH (1963) Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans Br Mycol Soc 46(2): 235-244.
Gupta ML, Prasad A, Ram M, Kumar S (2002) Effect of the vesicular–arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum on the essential oil yield related characters and nutrient acquisition in the crops of different cultivars of menthol mint (Mentha arvensis) under field conditions. Bioresour Technol 81(1), 77-79
Helgason T, Daniell TJ, Husband R, Fitter AH, Young JPW (1998) Ploughing up the wood-wide web?. Nat Biotechnol 394(6692): 431.
Husband R, Herre EA, Turner SL, Gallery R, Young JPW (2002) Molecular diversity of arbuscular mycorrhizal fungi and patterns of host association over time and space in a tropical forest. Mol Ecol 11(12): 2669-2678.
Jurenka JS (2009) Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: a review of preclinical and clinical research. Altern Med Rev 14(2): 141-151.
Khramov VA, Spasov AA, Samokhina MP (2008) Chemical composition of dry extracts of Gymnema sylvestre leaves. Pharm Chem J 42(1): 29-38.
Maheshwari RK, Singh AK, Gaddipati J, & Srimal RC (2006) Multiple biological activities of curcumin: a short review. Life Sci 78(18): 2081-2087.
Muthukumar T, Senthilkumar M, Rajangam M, Udaiyan K (2006) Arbuscular mycorrhizal morphology and dark septate fungal associations in medicinal and aromatic plants of Western Ghats, Southern India. Mycorrhiza 17(1): 11-24.
Panwar J, Tarafdar JC (2006) Distribution of three endangered medicinal plant species and their colonization with arbuscular mycorrhizal fungi. J Arid Environ 65(3): 337-350.
Reddy CA, Saravanan RS (2013) Polymicrobial multi-functional approach for enhancement of crop productivity. Adv Appl Microbiol 82: 53-113.
Rreddy MN, Devi MC, Sridevi NV (2003) Evaluation of turmeric cultivars for VAM colonization. Indian Phytopathol 56(4): 465-466.
Ruby AJ, Kuttan G, Babu KD, Rajasekharan KN, Kuttan R (1995) Anti-tumour and antioxidant activity of natural curcuminoids. Cancer Lett 94(1): 79-83.
Santos-González JC, Finlay RD, Tehler A (2007) Seasonal dynamics of arbuscular mycorrhizal fungal communities in roots in a seminatural grassland. Appl Environ Microbiol 73(17): 5613-5623.
Simon L, Lalonde M, Bruns TD (1992) Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Appl Environ Microbiol 58(1): 291-295.
Singh VK, Umar S, Ansari SA, Iqbal M (2008) Gymnema sylvestre for diabetics. J Herbs Spices Med Plants 14(1-2): 88-106.
Smith SE, Read DJ (2010) Mycorrhizal symbiosis, 3rd ed. Academic press. Page13.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol Biol Evol 30: 2725-2729.
Thapa T, De UK, Chakraborty B (2015) Association and root colonization of some medicinal plants with arbuscular mycorrhizal fungi. J Med Plant Stud 3: 25-35.
Zimare SB, Borde MY, Jite PK, Malpathak NP (2013) Effect of AM Fungi (Gf, Gm) on Biomass and Gymnemic Acid Content of Gymnema sylvestre (Retz) R. Br. ex Sm. Proceedings of the National Academy of Sciences, India - Section B: Biological Sciences 83(3): 439-445.
Zitterl-Eglseer K, Nell M, Lamien-Meda A, Steinkellner S, Wawrosch C, Kopp B, Novak J (2015) Effects of root colonization by symbiotic arbuscular mycorrhizal fungi on the yield of pharmacologically active compounds in Angelica archangelica L.. Acta Physiol Plant 37(2): 21.
Zubek S, Mielcarek S, Turnau K (2012) Hypericin and pseudohypericin concentrations of a valuable medicinal plant Hypericum perforatum L are enhanced by arbuscular mycorrhizal fungi. Mycorrhiza 22(2): 149-156.