Impact of metal contamination to the emergence of (multi) antibiotic resistant bacteria in Thai Nguyen, Vietnam

Pham Hoang Nam; Nguyen Quang Huy; Le Thi Thu Hang; Nguyen Thy Ngoc; Nguyen Thi Kieu Oanh; Bui Van Hoi; Nguyen Ngoc Lien; Nguyen Thi Thu Hien; Le Mai Huong; Nguyen Dinh Luyen; Nguyen Thi Huyen Thu; Tran Thi Hong Minh; Nguyen Tien Dat; Marie-Genevieve Dijoux-Franca

doi:10.15625/1811-4989/13509

Author affiliations

Authors

Pham Hoang Nam University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Nguyen Quang Huy University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Le Thi Thu Hang University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Nguyen Thy Ngoc University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Nguyen Thi Kieu Oanh University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Bui Van Hoi University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Nguyen Ngoc Lien University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Nguyen Thi Thu Hien Hanoi Open University, B101 Nguyen Hien Street, Ha Ba Trung District, Hanoi, Vietnam
Le Mai Huong Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Nguyen Dinh Luyen Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Nguyen Thi Huyen Thu Department of Pharmacy, Saint Paul Hospital, 12 Chu Van An Street, Ba Dinh District, Hanoi, Vietnam
Tran Thi Hong Minh Hanoi University of Natural Resources and Environment, 41A Phu Dien Road, North-Tu Liem District, Hanoi, Vietnam
Nguyen Tien Dat Center for Research and Technology Transfer, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Marie-Genevieve Dijoux-Franca UMR 5557, Ecologie Microbienne, CNRS, INRA, VetagroSup, UCBL, Université de Lyon, Villeurbanne, France

DOI:

https://doi.org/10.15625/1811-4989/13509

Keywords:

antibiotic, emergence, metal contamination, multidrug resistant, Pb

Abstract

Heavy metal contamination in the environment due to anthropogenic activities in industrial and agricultural areas represents stress for the living ecosystem including soil microorganisms. The adaptive response of bacterial communities to these pollutants can be observed through the increasing proportion of resistant bacteria. Our study aimed at evaluating the effect of metal contamination to the emergence of bacteria resistant to 3 common antibiotics, ie., gentamycin, sulfamethoxazole, and norfloxacin in Tan Long Pb-Zn mining site, Thai Nguyen province by a culture-dependent approach. The results showed that under moderated Pb contamination, a significant increase in prevalence of gentamycin-resistant bacteria was observed. Antibiotic susceptibility test showed 45% of isolates resistant to at least two antibiotics among gentamycin, norfloxacin, tetracyclin, imipenem, andsulfamethoxazole, and 10.4 % of isolates resistant tho three antibiotics. More detailed investigations in molecular analysis are needed to identify and genetically characterize of multi-drug resistant isolates as well as to better understand the link between metal resistance and (multi)resistance to antibiotics.

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