Genetic variation of the D-loop region of the Kinh ethnic group from the northern, central, and southern Vietnam

Duy La; Thao Dinh; Nguyen Thuy Duong

doi:10.15625/2615-9023/20952

Author affiliations

Authors

La Duc Duy Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Dinh Huong Thao Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Nguyen Thuy Duong Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/20952

Keywords:

D-loop, Kinh, mtDNA, variant, Vietnam

Abstract

The displacement-loop (D-loop) region is the 1122 bp sequence of the mitochondrial DNA (mtDNA) that plays an important role in mtDNA replication and transcription. This region is also a hotspot for mutations, making it a good candidate for genetic diversity studies, especially when combined with other unique characteristics of the mitochondria. The Kinh, which accounts for approximately 90% of the Vietnamese population, has been extensively used as a representative of the Vietnamese population. In this study, we sampled and sequenced the mtDNA of 31 Kinh individuals from Central Vietnam and 33 Kinh individuals from Southern Vietnam. In combination with 50 Northern Kinh mtDNA sequences from a previous study, the present study analyzed the diversity of the Kinh across Vietnam through the D-loop region. After aligning to the reference mtDNA sequence RSRS (Reconstructed Sapiens Reference Sequence), 134 unique variants were found in the D-loop region, including 34 variants found in all three populations, 25 variants found in two populations, and 75 variants unique to one Kinh population. Haplogroup calling using Haplogrep3 identified 60 haplogroups, with 23 out of 60 being absent in the Northern Kinh population. Interestingly, despite the genetic diversity of the Kinh, four haplogroups were found in all three populations (F1a1a, F1a2, B5a, M7b1a1+(16192T)). The pairwise genetic distance analysis showed that the Northern Kinh is distinguishable from the Central Kinh while the other pairing experienced the opposite trend. By sampling the Kinh group in three different regions, our results provided the basis for further genetic diversity analysis of the Kinh across Vietnam.

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References

Anderson S., Bankier A. T., Barrell B. G., De Bruijn M. H. L., Coulson A. R., Drouin J., Eperon I. C., Nierlich D. P., Roe B. A., Sanger F., Schreier P. H., Smith A. J. H., Staden R., Young I. G., 1981. Sequence and organization of the human mitochondrial genome. Nature, 290(5806): 457–465. doi: 10.1038/290457A0 DOI: https://doi.org/10.1038/290457a0

Brown W. M., George M., Wilson A. C., 1979. Rapid evolution of animal mitochondrial DNA. Proceedings of the National Academy of Sciences of the United States of America, 76(4): 1967–1971. https://doi.org/ 10.1073/PNAS.76.4.1967 DOI: https://doi.org/10.1073/pnas.76.4.1967

Hutchison C. A., Newbold J. E., Potter S. S., Edgell M. H., 1974. Maternal inheritance of mammalian mitochondrial DNA. Nature, 251(5475): 536–538. https://doi.org/ 10.1038/251536A0 DOI: https://doi.org/10.1038/251536a0

Michaels G. S., Hauswirth W. W., Laipis P. J., 1982. Mitochondrial DNA copy number in bovine oocytes and somatic cells. Developmental Biology, 94(1): 246–251. https://doi.org/10.1016/0012-1606(82)90088-4 DOI: https://doi.org/10.1016/0012-1606(82)90088-4

Hagström E., Freyer C., Battersby B. J., Stewart J. B., Larsson N. G., 2014. No recombination of mtDNA after heteroplasmy for 50 generations in the mouse maternal germline. Nucleic Acids Research, 42(2): 1111–1116. https://doi.org/ 10.1093/NAR/GKT969 DOI: https://doi.org/10.1093/nar/gkt969

Merriwether D. A., Clark A. G., Ballinger S. W., Schurr T. G., Soodyall H., Jenkins T., Sherry S. T., Wallace D. C., 1991. The structure of human mitochondrial DNA variation. Journal of Molecular Evolution, 33(6): 543–555. https://doi.org/10.1007/ BF02102807 DOI: https://doi.org/10.1007/BF02102807

Pakendorf B., Stoneking M., 2005. Mitochondrial DNA and human evolution. Annual Review of Genomics and Human Genetics, 6: 165–183. https://doi.org/ 10.1146/ANNUREV.GENOM.6.080604.162249 DOI: https://doi.org/10.1146/annurev.genom.6.080604.162249

Taanman J. W., 1999. The mitochondrial genome: structure, transcription, translation and replication. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1410(2): 103–123. https://doi.org/10.1016/ S0005-2728(98)00161-3 DOI: https://doi.org/10.1016/S0005-2728(98)00161-3

Chen Y., Wu W. J., Xing L. W., Zhang X. J., Wang J., Xia X. Y., Zhao R., Zhao R., 2023. Investigating the role of mitochondrial DNA D-loop variants, haplotypes, and copy number in polycystic ovary syndrome: implications for clinical phenotypes in the Chinese population. Frontiers in Endocrinology, 14: 1206995. https://doi.org/10.3389/ FENDO.2023.1206995/BIBTEX DOI: https://doi.org/10.3389/fendo.2023.1206995

Saha S. K., Saba A. Al, Hasib M., Rimon R. Al, Hasan I., Alam M. S., Mahmud I., Nabi A. H. M. N., 2021. Evaluation of D-loop hypervariable region I variations, haplogroups and copy number of mitochondrial DNA in Bangladeshi population with type 2 diabetes. Heliyon, 7(7): e07573. https://doi.org/10.1016/ J.HELIYON.2021.E07573 DOI: https://doi.org/10.1016/j.heliyon.2021.e07573

Torroni A., Schurr T. G., Cabell M. F., Brown M. D., Neel J. V., Larsen M., Smith D. G., Vullo C. M., Wallace D. C., 1993. Asian affinities and continental radiation of the four founding Native American mtDNAs. American Journal of Human Genetics, 53(3): 563.

Pischedda S., Barral-Arca R., Gómez-Carballa A., Pardo-Seco J., Catelli M. L., Álvarez-Iglesias V., Cárdenas J. M., Nguyen N. D., Ha H. H., Le A. T., Martinón-Torres F., Vullo C., Salas A., 2017. Phylogeographic and genome-wide investigations of Vietnam ethnic groups reveal signatures of complex historical demographic movements. Scientific Reports, 7(1): 1–15. https://doi.org/10.1038/s41598-017-12813-6 DOI: https://doi.org/10.1038/s41598-017-12813-6

Duong N.T., Phong N. Van, Ngoc N.T., Hai N. Van, 2020. Study on genetic variations of the D-loop region in three Vietnamese ethnic groups Kinh, Lolo and Lahu. Vietnam Journal of Biotechnology, 18(2): 231–238. https://doi.org/10.15625/1811-4989/18/2/15136 DOI: https://doi.org/10.15625/1811-4989/18/2/15136

Watson E., Forster P., Richards M., Bandelt H. J., 1997. Mitochondrial footprints of human expansions in Africa. American Journal of Human Genetics, 61(3): 691. https://doi.org/10.1086/515503 DOI: https://doi.org/10.1086/515503

Irwin J. A., Saunier J. L., Strouss K. M., Diegoli T. M., Sturk K. A., O’Callaghan J. E., Paintner C. D., Hohoff C., Brinkmann B., Parsons T. J., 2008. Mitochondrial control region sequences from a Vietnamese population sample. International Journal of Legal Medicine, 122(3): 257–259. https://doi.org/10.1007/ S00414-007-0205-3 DOI: https://doi.org/10.1007/s00414-007-0205-3

Derenko M., Malyarchuk B., Grzybowski T., Denisova G., Dambueva I., Perkova M., Dorzhu C., Luzina F., Hong K. L., Vanecek T., Villems R., Zakharov I., 2007. Phylogeographic Analysis of Mitochondrial DNA in Northern Asian Populations. American Journal of Human Genetics, 81(5): 1025. https://doi.org/ 10.1086/522933 DOI: https://doi.org/10.1086/522933

Abdulla M. A., Ahmed I., Assawamakin A., Bhak J., Brahmachari S. K., Calacal G. C., Chaurasia A., Chen C. H., Chen J., Chen Y. T., Chu J., Cutiongco-de La Paz E. M. C., De Ungria M. C. A., Delfin F. C., Edo J., Fuchareon S., Ghang H., Gojobori T., Han J., Ho S. F., Hoh B. P., Huang W., Inoko H., Jha P., Jinam T. A., Jin L., Jung J., Kangwanpong D., Kampuansai J., Kennedy G.C., Khurana P., Kim H.L., Kim K., Kim S., Kim W.Y., Kimm K., Kimura R., Koike T., Kulawonganunchai S., Kumar V., Lai P. S., Lee J. Y., Lee S., Liu E. T., Majumder P. P., Mandapati K. K., Marzuki S., Mitchell W., Mukerji M., Naritomi K., Ngamphiw C., Niikawa N., Nishida N., Oh B., Oh S., Ohashi J., Oka A., Ong R., Padilla C. D., Palittapongarnpim P., Perdigon H. B., Phipps M. E., Png E., Sakaki Y., Salvador J. M., Sandraling Y., Scaria V., Seielstad M., Sidek M. R., Sinha A., Srikummool M., Sudoyo H., Sugano S., Suryadi H., Suzuki Y., Tabbada K. A., Tan A., Tokunaga K., Tongsima S., Villamor L. P., Wang E., Wang Y., Wang H., Wu J. Y., Xiao H., Xu S., Yang J. O., Shugart Y. Y., Yoo H. S., Yuan W., Zhao G., Zilfalil B. A., 2009. Mapping human genetic diversity in Asia. Science (New York, N.Y.), 326(5959): 1541–1545. https://doi.org/ 10.1126/SCIENCE.1177074 DOI: https://doi.org/10.1126/science.1177074

Duong N. T., Macholdt E., Ton N. D., Arias L., Schröder R., Van Phong N., Thi Bich Thuy V., Ha N. H., Thi Thu Hue H., Thi Xuan N., Thi Phuong Oanh K., Hien L. T. T., Hoang N. H., Pakendorf B., Stoneking M., Van Hai N., 2018. Complete human mtDNA genome sequences from Vietnam and the phylogeography of Mainland Southeast Asia. Scientific Reports, 8(1): 1–13. https://doi.org/10.1038/s41598-018-29989-0. DOI: https://doi.org/10.1038/s41598-018-29989-0

Macholdt E., Arias L., Duong N. T., Ton N. D., Van Phong N., Schröder R., Pakendorf B., Van Hai N., Stoneking M., 2020. The paternal and maternal genetic history of Vietnamese populations. European Journal of Human Genetics, 28(5): 636–645. https://doi.org/10.1038/s41431-019-0557-4 DOI: https://doi.org/10.1038/s41431-019-0557-4

Li H., Cai X., Winograd-Cort E. R., Wen B., Cheng X., Qin Z., Liu W., Liu Y., Pan S., Qian J., Tan C. C., Jin L., 2007. Mitochondrial DNA diversity and population differentiation in southern East Asia. American Journal of Physical Anthropology, 134(4): 481–488. https://doi.org/10.1002/AJPA.20690 DOI: https://doi.org/10.1002/ajpa.20690

Auton A., Abecasis G. R., Altshuler D. M., Durbin R. M., Bentley D. R., Chakravarti A., Clark A. G., Donnelly P., Eichler E. E., Flicek P., Gabriel S. B., Gibbs R. A., Green E. D., Hurles M. E., Knoppers B. M., Korbel J. O., Lander E. S., Lee C., Lehrach H., Mardis E. R., Marth G. T., McVean G. A., Nickerson D. A., Schmidt J. P., Sherry S. T., Wang J., Wilson R. K., Boerwinkle E., Doddapaneni H., Han Y., Korchina V., Kovar C., Lee S., Muzny D., Reid J. G., Zhu Y., Chang Y., Feng Q., Fang X., Guo X., Jian M., Jiang H., Jin X., Lan T., Li G., Li J., Li Y., Liu S., Liu X., Lu Y., Ma X., Tang M., Wang B., Wang G., Wu H., Wu R., Xu X., Yin Y., Zhang D., Zhang W., Zhao J., Zhao M., Zheng X., Gupta N., Gharani N., Toji L. H., Gerry N. P., Resch A. M., Barker J., Clarke L., Gil L., Hunt S. E., Kelman G., Kulesha E., Leinonen R., McLaren W. M., Radhakrishnan R., Roa A., Smirnov D., Smith R. E., Streeter I., Thormann A., Toneva I., Vaughan B., Zheng-Bradley X., Grocock R., Humphray S., James T., Kingsbury Z., Sudbrak R., Albrecht M. W., Amstislavskiy V. S., Borodina T. A., Lienhard M., Mertes F., Sultan M., Timmermann B., Yaspo M. L., Fulton L., Ananiev V., Belaia Z., Beloslyudtsev D., Bouk N., Chen C., Church D., Cohen R., Cook C., Garner J., Hefferon T., Kimelman M., Liu C., Lopez J., Meric P., O’Sullivan C., Ostapchuk Y., Phan L., Ponomarov S., Schneider V., Shekhtman E., Sirotkin K., Slotta D., Zhang H., Balasubramaniam S., Burton J., Danecek P., Keane T. M., Kolb-Kokocinski A., McCarthy S., Stalker J., Quail M., Davies C. J., Gollub J., Webster T., Wong B., Zhan Y., Campbell C. L., Kong Y., Marcketta A., Yu F., Antunes L., Bainbridge M., Sabo A., Huang Z., Coin L. J. M., Fang L., Li Q., Li Z., Lin H., Liu B., Luo R., Shao H., Xie Y., Ye C., Yu C., Zhang F., Zheng H., Zhu H., Alkan C., Dal E., Kahveci F., Garrison E. P., Kural D., Lee W. P., Leong W. F., Stromberg M., Ward A. N., Wu J., Zhang M., Daly M. J., DePristo M. A., Handsaker R. E., Banks E., Bhatia G., Del Angel G., Genovese G., Li H., Kashin S., McCarroll S. A., Nemesh J. C., Poplin R. E., Yoon S. C., Lihm J., Makarov V., Gottipati S., Keinan A., Rodriguez-Flores J. L., Rausch T., Fritz M. H., Stütz A. M., Beal K., Datta A., Herrero J., Ritchie G. R. S., Zerbino D., Sabeti P.C., Shlyakhter I., Schaffner S. F., Vitti J., Cooper D. N., Ball E. V., Stenson P. D., Barnes B., Bauer M., Cheetham R. K., Cox A., Eberle M., Kahn S., Murray L., Peden J., Shaw R., Kenny E. E., Batzer M. A., Konkel M. K., Walker J. A., MacArthur D. G., Lek M., Herwig R., Ding L., Koboldt D. C., Larson D., Ye K., Gravel S., Swaroop A., Chew E., Lappalainen T., Erlich Y., Gymrek M., Willems T. F., Simpson J. T., Shriver M. D., Rosenfeld J. A., Bustamante C. D., Montgomery S. B., De La Vega F. M., Byrnes J. K., Carroll A. W., DeGorter M. K., Lacroute P., Maples B. K., Martin A. R., Moreno-Estrada A., Shringarpure S. S., Zakharia F., Halperin E., Baran Y., Cerveira E., Hwang J., Malhotra A., Plewczynski D., Radew K., Romanovitch M., Zhang C., Hyland F. C. L., Craig D. W., Christoforides A., Homer N., Izatt T., Kurdoglu A. A., Sinari S. A., Squire K., Xiao C., Sebat J., Antaki D., Gujral M., Noor A., Ye K., Burchard E. G., Hernandez R. D., Gignoux C. R., Haussler D., Katzman S. J., Kent W. J., Howie B., Ruiz-Linares A., Dermitzakis E. T., Devine S. E., Kang H. M., Kidd J. M., Blackwell T., Caron S., Chen W., Emery S., Fritsche L., Fuchsberger C., Jun G., Li B., Lyons R., Scheller C., Sidore C., Song S., Sliwerska E., Taliun D., Tan A., Welch R., Wing M. K., Zhan X., Awadalla P., Hodgkinson A., Li Y., Shi X., Quitadamo A., Lunter G., Marchini J. L., Myers S., Churchhouse C., Delaneau O., Gupta-Hinch A., Kretzschmar W., Iqbal Z., Mathieson I., Menelaou A., Rimmer A., Xifara D. K., Oleksyk T. K., Fu Y., Liu X., Xiong M., Jorde L., Witherspoon D., Xing J., Browning B.L., Browning S. R., Hormozdiari F., Sudmant P. H., Khurana E., Tyler-Smith C., Albers C. A., Ayub Q., Chen Y., Colonna V., Jostins L., Walter K., Xue Y., Gerstein M. B., Abyzov A., Balasubramanian S., Chen J., Clarke D., Fu Y., Harmanci A. O., Jin M., Lee D., Liu J., Mu X. J., Zhang J., Zhang Y., Hartl C., Shakir K., Degenhardt J., Meiers S., Raeder B., Casale F. P., Stegle O., Lameijer E. W., Hall I., Bafna V., Michaelson J., Gardner E. J., Mills R. E., Dayama G., Chen K., Fan X., Chong Z., Chen T., Chaisson M. J., Huddleston J., Malig M., Nelson B. J., Parrish N. F., Blackburne B., Lindsay S. J., Ning Z., Zhang Y., Lam H., Sisu C., Challis D., Evani U. S., Lu J., Nagaswamy U., Yu J., Li W., Habegger L., Yu H., Cunningham F., Dunham I., Lage K., Jespersen J. B., Horn H., Kim D., Desalle R., Narechania A., Sayres M. A. W., Mendez F. L., Poznik G. D., Underhill P. A., Mittelman D., Banerjee R., Cerezo M., Fitzgerald T. W., Louzada S., Massaia A., Yang F., Kalra D., Hale W., Dan X., Barnes K.C., Beiswanger C., Cai H., Cao H., Henn B., Jones D., Kaye J. S., Kent A., Kerasidou A., Mathias R., Ossorio P. N., Parker M., Rotimi C. N., Royal C. D., Sandoval K., Su Y., Tian Z., Tishkoff S., Via M., Wang Y., Yang H., Yang L., Zhu J., Bodmer W., Bedoya G., Cai Z., Gao Y., Chu J., Peltonen L., Garcia-Montero A., Orfao A., Dutil J., Martinez-Cruzado J. C., Mathias R. A., Hennis A., Watson H., McKenzie C., Qadri F., LaRocque R., Deng X., Asogun D., Folarin O., Happi C., Omoniwa O., Stremlau M., Tariyal R., Jallow M., Joof F. S., Corrah T., Rockett K., Kwiatkowski D., Kooner J., Hien T. T., Dunstan S. J., ThuyHang N., Fonnie R., Garry R., Kanneh L., Moses L., Schieffelin J., Grant D.S., Gallo C., Poletti G., Saleheen D., Rasheed A., Brooks L. D., Felsenfeld A. L., McEwen J. E., Vaydylevich Y., Duncanson A., Dunn M., Schloss J. A., 2015. A global reference for human genetic variation. Nature, 526(7571): 68–74. https://doi.org/10.1038/nature15393

Brandão A., Eng K. K., Rito T., Cavadas B., Bulbeck D., Gandini F., Pala M., Mormina M., Hudson B., White J., Ko T. M., Saidin M., Zafarina Z., Oppenheimer S., Richards M. B., Pereira L., Soares P., 2016. Quantifying the legacy of the Chinese Neolithic on the maternal genetic heritage of Taiwan and Island Southeast Asia. Human Genetics, 135(4): 363. https://doi.org/10.1007/S00439-016-1640-3 DOI: https://doi.org/10.1007/s00439-016-1640-3

Ngoc N. T., Trang N. B., Huy N. Q., Ton N. D., Duong N. T., 2018. Single nucleotide polymorphisms in the d-loop region of the mitochondrial genomes of individuals from two ethnic groups kinh and mang of austro-asiatic language family. Vietnam Journal of Biotechnology, 16(2): 231–240. doi: 10.15625/1811-4989/16/2/13432 DOI: https://doi.org/10.15625/1811-4989/16/2/13432

Maricic T., Whitten M., Pääbo S., 2010. Multiplexed DNA Sequence Capture of Mitochondrial Genomes Using PCR Products. PLoS ONE, 5(11): e14004. https://doi.org/10.1371/JOURNAL.PONE.0014004 DOI: https://doi.org/10.1371/journal.pone.0014004

Meyer M., Kircher M., 2010. Illumina sequencing library preparation for highly multiplexed target capture and sequencing. Cold Spring Harbor Protocols, 2010(6): pdb.prot5448. https://doi.org/10.1101/PDB.PROT5448 DOI: https://doi.org/10.1101/pdb.prot5448

Behar D. M., Van Oven M., Rosset S., Metspalu M., Loogväli E. L., Silva N. M., Kivisild T., Torroni A., Villems R., 2012. A “Copernican” Reassessment of the Human Mitochondrial DNA Tree from its Root. American Journal of Human Genetics, 90(4): 675. https://doi.org/ 10.1016/J.AJHG.2012.03.002 DOI: https://doi.org/10.1016/j.ajhg.2012.03.002

Li H., 2013. Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM.

Katoh K., Standley D. M., 2013. MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Molecular Biology and Evolution, 30(4): 772. https://doi.org/ 10.1093/MOLBEV/MST010 DOI: https://doi.org/10.1093/molbev/mst010

Schönherr S., Weissensteiner H., Kronenberg F., Forer L., 2023. Haplogrep 3 - an interactive haplogroup classification and analysis platform. Nucleic Acids Research, 51(W1): W263–W268. https://doi.org/10.1093/NAR/GKAD284 DOI: https://doi.org/10.1093/nar/gkad284

van Oven M., Kayser M., 2009. Updated comprehensive phylogenetic tree of global human mitochondrial DNA variation. Human Mutation, 30(2): E386–E394. https://doi.org/10.1002/HUMU.20921 DOI: https://doi.org/10.1002/humu.20921

Excoffier L., Lischer H. E. L., 2010. Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources. 10: 564–567. Evolutionary Bioinformatics Online. DOI: https://doi.org/10.1111/j.1755-0998.2010.02847.x

Lott M. T., Leipzig J. N., Derbeneva O., Michael Xie H., Chalkia D., Sarmady M., Procaccio V., Wallace D. C., 2013. mtDNA Variation and Analysis Using Mitomap and Mitomaster. Current Protocols in Bioinformatics, 44(1): 1–23. https://doi.org/10.1002/0471250953.BI0123S44 DOI: https://doi.org/10.1002/0471250953.bi0123s44

Pimenoff V. N., Comas D., Palo J. U., Vershubsky G., Kozlov A., Sajantila A., 2008. Northwest Siberian Khanty and Mansi in the junction of West and East Eurasian gene pools as revealed by uniparental markers. European Journal of Human Genetics : EJHG, 16(10):

1254–1264. doi: 10.1038/EJHG.2008.101 DOI: https://doi.org/10.1038/ejhg.2008.101

Comas D., Plaza S., Wells R. S., Yuldaseva N., Lao O., Calafell F., Bertranpetit J., 2004. Admixture, migrations, and dispersals in Central Asia: evidence from maternal DNA lineages. European Journal of Human Genetics : EJHG, 12(6): 495–504. https://doi.org/10.1038/ SJ.EJHG.5201160 DOI: https://doi.org/10.1038/sj.ejhg.5201160

Kutanan W., Kampuansai J., Srikummool M., Kangwanpong D., Ghirotto S., Brunelli A., Stoneking M., 2017. Complete mitochondrial genomes of Thai and Lao populations indicate an ancient origin of Austroasiatic groups and demic diffusion in the spread of Tai–Kadai languages. Human Genetics, 136(1): 85–98. https://doi.org/10.1007/S00439-016-1742-Y DOI: https://doi.org/10.1007/s00439-016-1742-y

Rajkumar R., Banerjee J., Gunturi H. B., Trivedi R., Kashyap V. K., 2005. Phylogeny and antiquity of M macrohaplogroup inferred from complete mt DNA sequence of Indian specific lineages. BMC Evolutionary Biology, 5: 26. https://doi.org/10.1186/1471-2148-5-26 DOI: https://doi.org/10.1186/1471-2148-5-26

Tanaka M., Cabrera V. M., González A. M., Larruga J. M., Takeyasu T., Fuku N., Guo L. J., Hirose R., Fujita Y., Kurata M., Shinoda K. I., Umetsu K., Yamada Y., Oshida Y., Sato Y., Hattori N., Mizuno Y., Arai Y., Hirose N., Ohta S., Ogawa O., Tanaka Y., Kawamori R., Shamoto-Nagai M., Maruyama W., Shimokata H., Suzuki R., Shimodaira H., 2004. Mitochondrial Genome Variation in Eastern Asia and the Peopling of Japan. Genome Research, 14(10a): 1832–50. https://doi.org/10.1101/GR.2286304 DOI: https://doi.org/10.1101/gr.2286304

Wen B., Li H., Gao S., Mao X., Gao Y., Li F., Zhang F., He Y., Dong Y., Zhang Y., Huang W., Jin J., Xiao C., Lu D., Chakraborty R., Su B., Deka R., Jin L., 2005. Genetic structure of Hmong-Mien speaking populations in East Asia as revealed by mtDNA lineages. Molecular Biology and Evolution, 22(3): 725–734. https://doi.org/10.1093/MOLBEV/MSI055 DOI: https://doi.org/10.1093/molbev/msi055

Woravatin W., Stoneking M., Srikummool M., Kampuansai J., Arias L., Kutanan W., 2023. South Asian maternal and paternal lineages in southern Thailand and the role of sex-biased admixture. PLOS ONE, 18(9): e0291547. https://doi.org/10.1371/ JOURNAL.PONE.0291547. DOI: https://doi.org/10.1371/journal.pone.0291547

Jaisamut K., Pitiwararom R., Sukawutthiya P., Sathirapatya T., Noh H., Worrapitirungsi W., Vongpaisarnsin K., 2023. Unraveling the mitochondrial phylogenetic landscape of Thailand reveals complex admixture and demographic dynamics. Scientific Reports, 13(1): 20396. https://doi.org/ 10.1038/S41598-023-47762-W DOI: https://doi.org/10.1038/s41598-023-47762-w

Peng M. S., He J. D., Liu H. X., Zhang Y. P., 2011. Tracing the legacy of the early Hainan Islanders--a perspective from mitochondrial DNA. BMC Evolutionary Biology, 11(46). https://doi.org/10.1186/ 1471-2148-11-46 DOI: https://doi.org/10.1186/1471-2148-11-46

Peng M. S., Quang H. H., Dang K. P., Trieu A. V., Wang H. W., Yao Y. G., Kong Q. P., Zhang Y. P., 2010. Tracing the Austronesian footprint in Mainland Southeast Asia: a perspective from mitochondrial DNA. Molecular Biology and Evolution, 27(10): 2417–2430. https://doi.org/10.1093/MOLBEV/MSQ131 DOI: https://doi.org/10.1093/molbev/msq131

Chandrasekar A., Kumar S., Sreenath J., Sarkar B. N., Urade B. P., Mallick S., Bandopadhyay S.S., Barua P., Barik S. S., Basu D., Kiran U., Gangopadhyay P., Sahani R., Prasad B. V. R., Gangopadhyay S., Lakshmi G. R., Ravuri R. R., Padmaja K., Venugopal P. N., Sharma M. B., Rao V. R., 2009. Updating phylogeny of mitochondrial DNA macrohaplogroup m in India: dispersal of modern human in South Asian corridor. PloS One, 4(10): e7447. https://doi.org/ 10.1371/JOURNAL.PONE.0007447 DOI: https://doi.org/10.1371/journal.pone.0007447

Ko A. M. S., Chen C. Y., Fu Q., Delfin F., Li M., Chiu H. L., Stoneking M., Ko Y. C., 2014. Early Austronesians: Into and Out Of Taiwan. American Journal of Human Genetics, 94(3): 426–36. https://doi.org/ 10.1016/J.AJHG.2014.02.003 DOI: https://doi.org/10.1016/j.ajhg.2014.02.003

Tam H. T., Linh D. P., 2022. How minor immigrants became the dominants: the case of the Kinh people migrating to the Central Highlands, Vietnam in the twentieth century. Social Identities, 28(5): 608–627. https://doi.org/10.1080/135046 30.2022.2131512 DOI: https://doi.org/10.1080/13504630.2022.2131512