Identification of a mutation in a Vietnamese family with Emery-Dreifuss Muscular Dystrophy using whole exome sequencing

Nguyen Thuy Duong; Dinh Huong Thao; Nguyen Thi Thao; Pham Van Anh; Miyake Noriko; Matsumoto Naomichi; Nong Van Hai

doi:10.15625/2615-9023/16742

Author affiliations

Authors

Nguyen Thuy Duong Institute of Genome Research
Dinh Huong Thao Institute of Genome Research
Nguyen Thi Thao Vietnam National Children’s Hospital, Vietnam
Pham Van Anh Vietnam National Children’s Hospital, Vietnam
Miyake Noriko Department of Human Genetics, Yokohama City University Graduate School of Medicine, Kanagawa, Japan. Department of Human Genetics, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
Matsumoto Naomichi Department of Human Genetics, Yokohama City University Graduate School of Medicine, Kanagawa, Japan
Nong Van Hai Institute of Genome Research

DOI:

https://doi.org/10.15625/2615-9023/16742

Keywords:

EDMD, LMNA, Sanger, WES, Vietnamese.

Abstract

Emery-Dreifuss muscular dystrophy (EDMD) is a degenerative neuromuscular disease associated with at least nine genes, including EMD, LMNA, FHL1, TMEM43, SUN1, SUN2, TTN, SYNE1, and SYNE2. Herein, we identified a heterozygous missense LMNA mutation (NM_170707.4: c.1357C>T,p.R453W) in three members of a Vietnamese family using whole-exome sequencing (WES), in which the proband was an 11-year-old girl presenting humeroperoneal muscle weaknesses and generalized contracture. Her father and one other relative also exhibited multiple signs of muscular atrophy and contracture. Sanger sequencing in the extended family verified the causative nature of this mutation, establishing a confirmed diagnosis of autosomal dominant Emery-Dreifuss muscular dystrophy (EDMD2). The clinical presentations of each patient in this study are different from each other, demonstrating the intrafamilial phenotypic variability of this mutation. Early identification of the underlying genetic course of the disease by sequencing, combined with clinical findings provides solid evidence to diagnosis process, genetic counseling and management strategy.

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