Antimicrobial and cytotoxic potential of endophytic actinomycetes isolated from Cinnamomum cassia Presl in Lai Chau Vietnam

Quach Ngoc-Tung, Vu Thi-Hanh Nguyen, Quyet Tien-Phi, Nguyen Quang Huy
Author affiliations


  • Quach Ngoc-Tung Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
  • Vu Thi-Hanh Nguyen Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
  • Quyet Tien-Phi Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
  • Nguyen Quang Huy University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam



Antimicrobial activities, biosynthesis genes, cytotoxicity, Cinnamomum cassia, endophytes, high mountainous region, Streptomyces.


New antimicrobials are urgently needed to combat the threat of multidrug-resistant bacteria worldwide. Our study aimed to access the antimicrobial and cytotoxic profiles of endophytic actinomycetes associated with Cinnamomum cassia Presl taken from a mountainous region in the Northwest of Vietnam. The antimicrobial activity, capability for anthracycline-like compounds production and presence of secondary metabolite-biosynthetic genes were consequently determined. Finally, the cytotoxicity of antibiotic-producing actinomycetes was carried out towards various cancer cell lines. A total of 81 actinomycetes were recovered from different organs (roots, stems, leaves) of Cinnamomum cassia, of which 20/81 isolates exhibited antimicrobial activities against at least one of nine microbial strains. The analysis of 16S rRNA genes indicated that antibiotic-producing isolates were grouped into 4 genera Streptomyces, Micromonospora, Saccharothrix, and Microbacterium, among which Streptomyces was the most prevalent. The presence of biosynthetic genes pks-I, pks-II or nrps was detected in 17/20 of isolates. Five strains of Streptomyces griseorubens LCQ8, Streptomyces variabilis LCQ43, Streptomyces californicus LCQ44, Streptomyces fragilis LCQ75, and Streptomyces beijiangensis LCQ77 exhibited broad-spectrum antimicrobial activity against various pathogens with the MIC values ranging from 16 to 256 µg/mL. Furthermore, characterized strains showed considerable cytotoxicity against MCF-7 cell lines with a 50% inhibition concentration of crude culture extracts of less than 30 µg/mL. The findings demonstrated that Streptomyces species isolated from Cinnamomum cassia in mountainous regions hold the potential for growth inhibition of human disease agents.


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How to Cite

Quach , N.-T., Vu , T.-H. N., Phi, Q. T., & Nguyen , Q. H. (2024). Antimicrobial and cytotoxic potential of endophytic actinomycetes isolated from <i>Cinnamomum cassia</i> Presl in Lai Chau Vietnam. Academia Journal of Biology, 46(2), 121–131.




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