Profile of the gut microbial composition in <i>Apis mellifera</i> larvae collected in Ha Noi

Van Quyen Dong; Thuy Duong Bui Thi; Thi Lanh Pham; Thi Hoa Nguyen; Quang Huy Nguyen; Thi Thu Ha; Hong Thai Pham

doi:10.15625/2615-9023/20165

Author affiliations

Authors

Dong Van Quyen Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Bui Thi Thuy Duong Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Pham Thi Lanh Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Nguyen Thi Hoa Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Nguyen Quang Huy University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Ha Thi Thu Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Pham Hong Thai Research Center for Tropical Bees and Beekeeping, Vietnam National University of Agriculture, Ha Noi, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/20165

Keywords:

Apis mellifera, honeybees, Lactobacillus, larvae, microbiome.

Abstract

The gut microbiota plays a crucial role in food digestion, enhances the host's immune system, and against pathogens. Numerous studies have been conducted on the microbiota of insects in general and honeybees in particular. However, studies have primarily focused on adult honeybees, with fewer studies dedicated to larvae. Despite being within the hive, honeybee larvae still possess their distinct microbiota. To gain a deeper understanding of the microbiota in the larvae of Apis mellifera honeybees, the larva from honeybee colonies collected in Ha Noi, Vietnam was investigated. Next-generation sequencing (NGS) targeting the 16S rRNA gene was employed for microbiome analysis. Results revealed the presence of 5 phyla including Proteobacteria (70.43%), Actinobacteria (1.16%), Firmicutes (20.87%), Bacteroidetes (2.72%), and Chloroflexi (2%). Representative genera included Bombella (29.97%), Lactobacillus (14.91%), Gilliamella (9.59%), Frischella (4.69%), Snodgrassella (3.85%), and Marinobacter (1.21%). Further characterized species composition in the sample we identified the prevalence of Bifidobacterium intestini (29.96%), Gilliamella apicola (8.08%), Frischella perrara (4.55%), Lactobacillus kimbladii (2.85%), Lactobacillus plantarum (2.80%), Snodgrassella alvi (2.77%), Lactobacillus mellis (2.59%), Lactobacillus_uc (unclassified or not yet classified to species, 2.19%), Lactobacillus kunkeei (1.43%), and Lactobacillus melliventris (1.31%). Understanding these microbial dynamics is crucial for developing strategies to support honeybee health and mitigate the challenges posed by factors, such as pesticides, environmental pollution, and honeybee diseases.

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