Screening and characterization of paclitaxel-producing fungus \(\textit{Talaromyces wortmannii}\) WQF18 isolated from \(\textit{Cephalotaxus mannii }\)Hook. f..

Quach Ngoc Tung; Vu Thi Hanh Nguyen; Pham Quynh Anh; Tran Hong Quang; Chu Hoang Ha; Phi Quyet Tien

doi:10.15625/2615-9023/18002

Author affiliations

Authors

Quach Ngoc Tung Institute of Biotechnology, VAST, Vietnam
Vu Thi Hanh Nguyen Institute of Biotechnology, VAST, Vietnam
Pham Quynh Anh Institute of Biotechnology, VAST, Vietnam
Tran Hong Quang Institute of Marine Biochemistry, VAST, Vietnam
Chu Hoang Ha Institute of Biotechnology, VAST, Vietnam
Phi Quyet Tien Institute of Biotechnology, VAST, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/18002

Keywords:

Cephalotaxus mannii, cytotoxicity, dbat, endophytic fungi, paclitaxel, Talaromyces wortmannii.

Abstract

Cephalotaxus mannii Hook. f. is a rare evergreen conifer native listed in the International Union for Conservation of Nature (IUCN) Red List, which is utilized for leukemia treatment. Although endophytic fungi from C. mannii was reported before, their cytotoxic property has not been revealed yet. In the present study, a total of 7 endophytic fungi were isolated from C. mannii collected in Ha Giang province, Vietnam, among which the isolate WQF18 was active against 5 tested pathogens with inhibition zones ranging from 18.0 ± 0.7 to 25.0 ± 0.4 mm. In addition, only ethyl acetate extract of isolate WQF18 showed cytotoxicity on A549 and MCF7 cell lines with IC₅₀ values of 69.6 ± 2.3 µg/mL and 78.6 ± 1.6 µg/mL, respectively. PCR-based molecular screening revealed that the positive hits for both 10-deacetylbaccatin III-10-O-acetyl transferase (dbat) and taxadiene synthase (ts) genes involved in paclitaxel biosynthesis were only observed in the WQF18 isolate. Based on morphological and molecular identification, the WQF18 isolate was identified as Talaromyces wortmannii WQF18. The presence of paclitaxel in T. wortmannii WQF18 was further confirmed by dbat sequence alignment, phenotypic, and HPLC-DAD analysis. To the best of our knowledge, this is the first report demonstrating the paclitaxel-producing capability of endophytic fungi from C. mannii. These findings provide a new platform for deciphering paclitaxel biosynthesis of endophytic fungi from non-Taxus plants and further paclitaxel production.

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