Efficacy of biosynthesizing folate, riboflavin and typical probiotic traits reveal the potential use of \(\textit{Lactobacillus plantarum}\) LCN13 as a feed additive for swine farming

Vu Thi Hanh Nguyen; Quach Ngoc Tung; Bui Thi Lien; Nguyen  Huyen Trang; Nguyen Van The; Nguyen Thi Thanh Loi; Chu Hoang Ha; Phi Quyet Tien

doi:10.15625/2615-9023/16628

Author affiliations

Authors

Vu Thi Hanh Nguyen Institute of Biotechnology, VAST, Vietnam
Quach Ngoc Tung Institute of Biotechnology, VAST, Vietnam
Bui Thi Lien Institute of Biotechnology, VAST, Vietnam
Nguyen Huyen Trang Institute of Biotechnology, VAST, Vietnam
Nguyen Van The Institute of Biotechnology, VAST, Vietnam
Nguyen Thi Thanh Loi Institute of Biotechnology, VAST, Vietnam
Chu Hoang Ha Institute of Biotechnology, VAST, Vietnam
Phi Quyet Tien Institution of Biotechnology, Vietnam Academy of Science and Technology

DOI:

https://doi.org/10.15625/2615-9023/16628

Keywords:

Lactic acid bacteria, Lactobacillus plantarum, probiotics, riboflavin, swine farming.

Abstract

Lactic acid bacteria have been advocated as probiotics to replace antibiotic growth promoters, improve growth performance, and reduce cost production in swine farming. The aim of this study is to identify and evaluate the probiotic traits of strain LCN13 isolated from traditionally fermented feed for swine. Thirty-five bacterial isolates with different morphological characteristics were isolated, among which 9 isolates showed the ability to produce lactic acid and antibacterial activity against pathogenic bacteria. Among them, isolate LCN13 exhibited a strong capacity to produce lactic acid (18.5 ± 0.31 g/L), inhibited gastrointestinal pathogens such as Salmonella typhimurium ATCC 14028 (18.3 ± 1.52 mm), Escherichia coli ATCC 11105 (24.7 ± 2.14 mm), and Staphylococcus epidermidis ATCC 35984 (31.6 ± 2.93 mm), and produced 182 ng/mL folate and 233 ng/mL riboflavin as measured by LC-MS/MS after 24 hours of incubation. Based on morphological, biochemical, and 16S rRNA gene analysis, the isolate LCN13 was identified as Lactobacillus plantarum. Phenotypic analysis revealed that L. plantarum LCN13 showed remarkable resistance to 1.2% ox-bile salt, 2.0 mM H₂O₂, and pH 3.0. In addition, the ability to produce high levels of folate (253.6 ± 10.7 ng/mL) and riboflavin (312.0 ± 12.2 ng/mL) after 48 hours was exploited for the first time in the L. plantarum. Taken together, L. plantarum LCN13 might serve as a potential probiotic candidate for animal farming.

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