INORGANIC NITROGEN REMOVAL IN TROPICALLY COASTAL AQUACULTURE PONDS USING INTEGRATED BIOREMEDIATION
Keywords:Coastal aquaculture, inorganic nitrogen, bioremediation, nitrifying bacteria, denitrifying bacteria.
AbstractBioremediation technology has been emergently considered as an economic and environmental-friendly manner to treat inorganic nitrogen in tropically coastal aquaculture ponds. Two laboratory scale experiments were conducted to examine efficacy of nitrogen removal of some bioremediators. The results indicated that total ammonia nitrogen (TAN) and nitrite concentrations of treatments with inoculation of the nitrifying bacterial cultures were significantly lower than those of the treatments without inoculation just after three days and one day of experiment, respectively. TAN in treatments with inoculation was removed at 76.0% while that without inoculation was just removed at 53.7%. Nitrite in treatments without inoculation increased duration of first 20 experiment days while nitrite in treatments with inoculation decreased to 50% just after one day. For treatments with inoculation of denitrifying bacterial cultures, effect of nitrate removal was clear within one day after beginning of the experiment. The present of macroalgae Gracilaria sp. promoted TAN and nitrite removal after two to three days of the experiment. Moreover, the addition of CaCO3 could enhance TAN and nitrite removal in comparison to the control treatments.
Chin, T. S., and Chen, J. C., 1987. Acute toxicity of ammonia to larvae of the tiger prawn, Penaeus monodon. Aquaculture, 66(3-4), 247-253.
Muir, P. R., Sutton, D. C., and Owens, L., 1991. Nitrate toxicity to Penaeus monodon protozoea. Marine Biology, 108(1), 67-71.
Boyd, C. E., 1998. Water quality for pond aquaculture. Alabama Agriculture Experimental Station, Auburn University, Alabama. 37 pp.
Gross, A., Abutbul, S., and Zilberg, D., 2004. Acute and Chronic Effects of Nitrite on white shrimp, Litopenaeus vannamei, cultured in low‐salinity brackish water. Journal of the World Aquaculture Society, 35(3), 315-321.
Chopin, T., Buschmann, A. H., Halling, C., Troell, M., Kautsky, N., Neori, A., Kraemer, G. P., Zertuche-González, J. A., Yarish, C., and Neefus, C., 2001. Integrating seaweeds into marine aquaculture systems: a key toward sustainability. Journal of Phycology, 37(6), 975-986.
Burford, M. A., Costanzo, S. D., Dennison, W. C., Jackson, C. J., Jones, A. B., McKinnon, A. D., Preston, N. P., and Trott, L. A., 2003. A synthesis of dominant ecological processes in intensive shrimp ponds and adjacent coastal environments in NE Australia. Marine Pollution Bulletin, 46(11), 1456-1469.
Worm, B., Sandow, M., Oschlies, A., Lotze, H. K., and Myers, R. A., 2005. Global patterns of predator diversity in the open oceans. Science, 309(5739), 1365-1369.
Anh, P. T., Kroeze, C., Bush, S. R., and Mol, A. P., 2010. Water pollution by intensive brackish shrimp farming in south-east Vietnam: Causes and options for control. Agricultural Water Management, 97(6), 872-882.
Parker, A. E., Dugdale, R. C., and Wilkerson, F. P., 2012. Elevated ammonium concentrations from wastewater discharge depress primary productivity in the Sacramento river and the Northern San Francisco estuary. Marine Pollution Bulletin, 64(3), 574-586.
Isobe, K., and Ohte, N., 2014. Ecological perspectives on microbes involved in N-cycling. Microbes and Environments, 29(1), 4-16.
Prabhu, N. M., Nazar, A. R., Rajagopal, S., and Khan, S. A., 1999. Use of probiotics in water quality management during shrimp culture. Journal of Aquaculture in the Tropics, 14(3), 227-233.
Shariff, M., Yusoff, F. M., Devaraja, T. N., and Rao, P. S., 2001. The effectiveness of a commercial microbial product in poorly prepared tiger shrimp, Penaeus monodon (Fabricius), ponds. Aquaculture Research, 32(3), 181-187.
Sambasivam, S., Chandran, R., and Khan, S. A., 2003. Role of probiotics on the environment of shrimp pond. Journal of Environmental Biology, 24(1), 103-106.
Rombaut, G., Grommen, R., Zizhong, Q., Vanhoof, V., Suantika, G., Dhert, P., Sorgeloos, P., and Verstraete, W., 2003. Improved performance of an intensive rotifer culture system by using a nitrifying inoculum (ABIL). Aquaculture Research, 34(2), 165-174.
Janeo, R. L., Corre, V. L., and Sakata, T., 2009. Water quality and phytoplankton stability in response to application frequency of bioaugmentation agent in shrimp ponds. Aquacultural Engineering, 40(3), 120-125.
Barik, P., Vardia, H. K., and Gupta, S. B., 2011. Bioremediation of ammonia and nitrite in polluted water. International Journal of Fisheries and Aquaculture, 3(7), 136-142.
Stephenson, D., and Stephenson, T., 1992. Bioaugmentation for enhancing biological wastewater treatment. Biotechnology Advances, 10(4), 549-559.
Gatesoupe, F. J., 1999. The use of probiotics in aquaculture. Aquaculture, 180(1), 147-165.
Diab, S., and Shilo, M., 1988. Effect of adhesion to particles on the survival and activity of Nitrosomonas sp. and Nitrobacter sp. Archives of Microbiology, 150(4), 387-393.
Shan, H., and Obbard, J., 2001. Ammonia removal from prawn aquaculture water using immobilized nitrifying bacteria. Applied Microbiology and Biotechnology, 57(5), 791-798.
Manju, N. J., Deepesh, V., Achuthan, C., Rosamma, P., and Singh, I. B., 2009. Immobilization of nitrifying bacterial consortia on wood particles for bioaugmenting nitrification in shrimp culture systems. Aquaculture, 294(1), 65-75.
Chen, S., Ling, J., and Blancheton, J. P., 2006. Nitrification kinetics of biofilm as affected by water quality factors. Aquacultural Engineering, 34(3), 179-197.
Neori, A., Chopin, T., Troell, M., Buschmann, A. H., Kraemer, G. P., Halling, C., Shpigel, M., and Yarish, C., 2004. Integrated aquaculture: rationale, evolution and state of the art emphasizing seaweed biofiltration in modern mariculture. Aquaculture, 231(1), 361-391.
Marinho-Soriano, E., Azevedo, C. A. A., Trigueiro, T. G., Pereira, D. C., Carneiro, M. A. A., and Camara, M. R., 2011. Bioremediation of aquaculture wastewater using macroalgae and Artemia. International biodeterioration and Biodegradation, 65(1), 253-257.
Raja, S., Dinesh, K. P. B., Kesavan, K., Kodungallur, T., and Thrissur, K., 2014. Bioremediation by using of microbes and algae with special reference to coastline environment. Int. J. Biosci. Nanosci, 1(6), 130-140.
Watson, S. W., Bock, E., Harms, H., Koops, H., and Hooper, A. B., 1989. Nitrifying bacteria, in Bergey's Manual of Systematic Bacteriology (eds Stanley, J. T., Bryant, M. P., Pfennig, N., and Holt, J. G.), Williams & Wilkins, Baltimore, pp. 1808-1834.
Solorzano, L., 1969. Determination of ammonia in natural waters by the phenolhypochlorite method. This research was fully supported by US Atomic Energy Commission Contract No. ATS (11‐1) GEN 10, PA 20. Limnology and oceanography, 14(5), 799-801.
Strickland, J. D., and Parsons, T. R., 1972. A practical handbook of seawater analysis. Fisheries Research Board of Canada, Ottawa.
Grommen, R., Van Hauteghem, I., Van Wambeke, M., and Verstraete, W., 2002. An improved nitrifying enrichment to remove ammonium and nitrite from freshwater aquaria systems. Aquaculture, 211(1), 115-124.
Van Rijn, J., Tal, Y., and Schreier, H. J., 2006. Denitrification in recirculating systems: theory and applications. Aquacultural Engineering, 34(3), 364-376.
Tal, Y., Schreier, H. J., Sowers, K. R., Stubblefield, J. D., Place, A. R., and Zohar, Y., 2009. Environmentally sustainable land-based marine aquaculture. Aquaculture, 286(1), 28-35.
Müller-Belecke, A., Zienert, S., Thürmer, C., Kaufhold, S., and Spranger, U., 2013. The “self cleaning inherent gas denitrification-reactor” for nitrate elimination in RAS for pike perch (Sander lucioperca) production. Aquacultural Engineering, 57, 18-23.
McGowan, C. J., 2007. Nitrification Potential and Nitrate Reduction to Ammonium in a Nitrogen-Polluted Estuary Doctoral dissertation, Brown University, (date). 29 pp.
Ando, Y., Nakagawa, T., Takahashi, R., Yoshihara, K., and Tokuyama, T., 2009. Seasonal changes in abundance of ammonia-oxidizing archaea and ammonia-oxidizing bacteria and their nitrification in sand of an eelgrass zone. Microbes and Environments, 24(1), 21-27.
Chen, G. H., Ozaki, H., and Terashima, Y., 1989. Modelling of the simultaneous removal of organic substances and nitrogen in a biofilm. Water Science and Technology, 21(8-9), 791-804.
Gujer, W., and Boller, M., 1986. Design of a nitrifying tertiary trickling filter based on theoretical concepts. Water Research, 20(11), 1353-1362.