Ciguatoxin-like toxin in mackerel <i>Acanthocybium soladria</i> in Vietnam

Dao Viet Ha; Phan Bao Vy

doi:10.15625/1859-3097/19/4A/14611

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Authors

Dao Viet Ha Journal of Marine Science and Technology
Phan Bao Vy

DOI:

https://doi.org/10.15625/1859-3097/19/4A/14611

Keywords:

Mackerel, ciguatera, food poisoning, Vietnam.

Abstract

Two processed mackerel products were sent to VAST keylab on “Food and Environmental Safety” in Institute of Oceanography for Ciguatoxin criteria as exported seafood requirement. The specimens were caught from offshore region of Vietnam South-Eastern sea in April 2019. By DNA sequencing of COI gene, both specimens were identified as Acanthocybium solandri, one of high commercial mackerel species which is well-known to be distributed in Central and South-Eastern sea of Vietnam. In mouse bioassay, both extracts from 2 samples exhibited acute toxicity, which killed tested mice within 6 and 15 hours after i.p. injection. In SIM LC/MS-MS analysis, the extracts showed the peak with retention time closed to that in CTX-1B standard in both cases of optimized and non-optimized solid phases. The result suggested that at least there was occurrence of CTX-like toxin in these mackerel specimens. The equivalent toxin levels were estimated as 0.51 ng/g and 0.84 ng/g; 5 and 8 times higher than regulation limit for CTXs in seafood product (0.1 ng CTX-1B/g). Further verification is in progress to confirm toxin in these mackerel samples in Vietnam. However, this data is the first alert on potential poisoning by consumption of mackerel, not only in Vietnam but also in Asia.

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References

Ahmed, F. E., 1991. Seafood safety. National Academy Press. 768 p.

Ha, D. V., Uesugi, A., Uchida, H., Ky, P. X., Minh, D., Watanabe, R., Matsushima, R., Oikawa, H., Nagai, S., Iwataki, M., Fukuyo, Y., and Suzuki, T., 2018. Identification of causative ciguatoxins in red snappers Lutjanus bohar implicated in ciguatera fish poisonings in Vietnam. Toxins, 10(10), 420.

Ward, R. D., Zemlak, T. S., Innes, B. H., Last, P. R., and Hebert, P. D., 2005. DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462), 1847–1857. doi: 10.1098/rstb.2005.1716.

Kumar, S., Stecher, G., and Tamura, K., 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular biology and evolution, 33(7), 1870–1874.

Darriba, D., Taboada, G. L., Doallo, R., and Posada, D., 2012. jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9(8), 772.

Oshiro, N., Yogi, K., Asato, S., Sasaki, T., Tamanaha, K., Hirama, M., Yasumoto, T., and Inafuku, Y., 2010. Ciguatera incidence and fish toxicity in Okinawa, Japan. Toxicon, 56(5), 656–661. https://doi.org/10.1016/j.toxicon.2009.05.036.

Lewis, R. J., and Sellin, M., 1992. Multiple ciguatoxins in the flesh of fish. Toxicon, 30(8), 915–919. https://doi.org/ 10.1016/0041-0101(92)90390-Q.

Yogi, K., Oshiro, N., Inafuku, Y., Hirama, M., and Yasumoto, T., 2011. Detailed LC-MS/MS analysis of ciguatoxins revealing distinct regional and species characteristics in fish and causative alga from the Pacific. Analytical Chemistry, 83(23), 8886–8891. DOI: 10.1021/ac200799j.

Suzuki, T., Ha, D. V., Uesugi, A., and Uchida, H., 2017. Analytical challenges to ciguatoxins. Current Opinion in Food Science, 18, 37–42.

Đào Việt Hà. 2017. Độc tính của mẫu cá hồng gây ngộ độc thực phẩm tại Việt Nam. Tạp chí Khoa học và Công nghệ biển, 17(4A), 331–337.

EFSA Panel on Contaminants in the Food Chain, 2010. Scientific Opinion on marine biotoxins in shellfish-Emerging toxins: Ciguatoxin group. EFSA Journal, 8(6), 1627. https://doi.org/10.2903/j.efsa.2010. 1627.

Oshiro, N., Matsuo, T., Sakugawa, S., Yogi, K., Matsuda, S., Yasumoto, T., and Inafuku, Y., 2011. Ciguatera Fish Poisoning on Kakeroma Island, Kagoshima Prefecture, Japan. Tropical Medicine & Health, 39(2), 53–57. DOI: 10.2149/ tmh.2011-03.

Yogi, K., Oshiro, N., Matsuda, S., Sakugawa, S., Matsuo, T., and Yasumoto, T., 2013. Toxin profiles in fish implicated in ciguatera fish poisoning in Amami and Kakeroma islands, Kagoshima prefecture, Japan. Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan, 54(6), 385–391. DOI: 10.3358/shokueishi. 54.385.