Some methods to stain cartilage and bone of vertebrates and application on small fish in museum of oceanography

Nguyen Thi My Ngan, Bui Quang Nghi, Nguyen Kha Phu, Hoang Xuan Ben
Author affiliations

Authors

  • Nguyen Thi My Ngan Journal of Marine Science and Technology
  • Bui Quang Nghi
  • Nguyen Kha Phu
  • Hoang Xuan Ben

DOI:

https://doi.org/10.15625/1859-3097/19/4A/14594

Keywords:

Staining of cartilage and bone, Amphiprion ocellaris, vertebrates.

Abstract

In a natural scientific museum as Museum of Oceanography, a specimen should be not only awesome but also scientific in order to attract visitors and introduce knowledge to them. One of the methods satisfying those requirements is “Method of staining cartilages and bones for small vertebrates”. This method was applied to handle small fish: Anchovies (Encrasicholina devisi (Whitley, 1940)), amphiprions (Amphiprion ocellaris (Cuvier 1830)) and ponyfish (Leiognathus sp.), which is highly efficient, not only used in studying fish bones and cartilages but also attractive to visitors. This paper also gives details on experiences during handling process of this method.

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References

Pramod, K. L., and Vaswani, V. R., 2011. Museum preservation of skeleton of fetus & small vertebrates. Recent Research in Science and Technology, 3(2), 54–58.

Armbruster, J. W. 1989. Clearing and staining method. (http://www.aquam-edia.org/FileLibrary/27/Diagnostics%20-­‐%20Staining.pdf).

Dingerkus, G., and Uhler, L. D., 1977. Enzyme clearing of alcian blue stained whole small vertebrates for demonstration of cartilage. Stain Technology, 52(4), 229–232.

Bensimon-Brito, A., Cardeira, J., Dionísio, G., Huysseune, A., Cancela, M. L., and Witten, P. E., 2016. Revisiting in vivo staining with alizarin red S-a valuable approach to analyse zebrafish skeletal mineralization during development and regeneration. BMC Developmental Biology, 16(1), 1–10.

Lewis, L. M., Lall, S. P., and Witten, P. E., 2004. Morphological descriptions of the early stages of spine and vertebral development in hatchery-reared larval and juvenile Atlantic halibut (Hippoglossus hippoglossus). Aquaculture, 241(1–4), 47–59. doi: 10.1016/j.aquaculture.2004. 08.018.

Gavaia, P. J., Sarasquete, C., and Cancela, M. L., 2000. Detection of mineralized structures in early stages of development of marine Teleostei using a modified alcian blue-alizarin red double staining technique for bone and cartilage. Biotechnic & Histochemistry, 75(2), 79–84.

Taylor, W. R., 1967. Outline of a method of claring tissues with pancreatic enzymes and staining bones of small vertebrates. Turtox News, 45, 308–309.

Potthoff, T., 1984. Clearing and staining techniques. In: Moser, H. G. (Ed.), Ontog-eny and Systematics of Fishes. Special Publication-American Society of Ichthyolo-gists and Herpetologists,

Vol. 1. Allen Press, Lawrence, KS, USA,

pp. 35–37.

Kawamura, K., and Hosoya, K., 1991. A modified double staining technique for making a transparent fish-skeletal specimen. Bulletin of National Research Institute of Aquaculture (Japan), 20,

–18.

Fjelldal, P. G., Totland, G. K., Hansen, T., Kryvi, H., Wang, X., Søndergaard, J. L., and Grotmol, S., 2013. Regional changes in vertebra morphology during ontogeny reflect the life history of A tlantic cod (G adus morhua L.). Journal of Anatomy, 222(6), 615–624.

Song, J., and Parenti, L. R., 1995. Clearing and staining whole fish specimens for simultaneous demonstration of bone, cartilage, and nerves. Copeia, 114–118.

Rosa-Molinar, E., Proskocil, B. J., Ettel, M., and Fritzsch, B., 1999. Whole-mount procedures for simultaneous visualization of nerves, neurons, cartilage and bone. Brain Research Protocols, 4(2), 115–123.

Taylor, W. R., 1985. Revised procedures for staining and clearing small fishes and other vertebrates for bone and cartilage study. Cybium, 9, 107–119.

Walker, M. B., and Kimmel, C. B., 2007. A two-color acid-free cartilage and bone stain for zebrafish larvae. Biotechnic & Histochemistry, 82(1), 23–28.

Helland, I. S., 1988. On the structure of partial least squares regression. Communications in statistics -Simulation and Computation, 17(2), 581–607.

Johnson, G. D., and Anderson Jr, W. D., 1993. Proceedings of the Symposium on Phylogeny of Percomorpha, June 15-17, 1990, held in Charleston, South Carolina at the 70th Annual Meeting of the American Society of Ichthyologists and Herpetologists.

Summer, A., 2013. On the clarity of animals: the art and science of clearing and staining. http://www.southernfried-science.com/on-the-clarity-of-animals-the-art-and-science-of-clearing-and-staining. Truy cập ngày 12/9/2018.

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Published

08-11-2019

How to Cite

Ngan, N. T. M., Nghi, B. Q., Phu, N. K., & Ben, H. X. (2019). Some methods to stain cartilage and bone of vertebrates and application on small fish in museum of oceanography. Vietnam Journal of Marine Science and Technology, 19(4A), 115–122. https://doi.org/10.15625/1859-3097/19/4A/14594

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