Development of a fluorescence correlation spectroscopy instrument and its application in sizing quantum dot nanoparticles

Nguyen Thi Thanh Bao; Dinh Van Trung

doi:10.15625/0868-3166/25/1/5670

Development of a fluorescence correlation spectroscopy instrument and its application in sizing quantum dot nanoparticles

Nguyen Thi Thanh Bao, Dinh Van Trung

Author affiliations

Authors

Nguyen Thi Thanh Bao Institute of Physics, Vietnam Academy of Science and Technology
Dinh Van Trung Institute of Physics, Vietnam Academy of Science and Technology

DOI:

https://doi.org/10.15625/0868-3166/25/1/5670

Keywords:

fluorescence correlation spectroscopy, single molecule detection, quantum dots, photon counting

Abstract

Fluorescence correlation spectroscopy is a relatively new technique to measure and quantify the statistical fluctuations of the fluorescence signal from the measurement volume. Combining with sensitive detection method and confocal microscopy, the FCS technique has become a powerful tool in studying the dynamic properties of nanoparticles at single particle level. In this paper we present the construction of a highly sensitive FCS instrument and the measurement results from a sample of semiconductor quantum dots. We provide the analysis procedure for determining the hydrodynamic radius of the quantum dots and compare the results with that obtained directly from electron microscope imaging. The good agreement indicates the reliability of the FCS technique and open the way for further applications of this technique in studying nanoparticles.

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Published

21-04-2015

How to Cite

[1]

N. T. T. Bao and D. V. Trung, Development of a fluorescence correlation spectroscopy instrument and its application in sizing quantum dot nanoparticles, Comm. Phys. 25 (2015) 59. DOI: https://doi.org/10.15625/0868-3166/25/1/5670.

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Issue

Vol. 25 No. 1 (2015)

Section

Papers

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