Screening for genes encoding protease inhibitor from metagenome of sponge-associated microorganisms in Quang Tri, Vietnam

Tran Thi Hong, Pham Viet Cuong, Nguyen Thi Kim Cuc


In recent years, metagenomics-based method to isolate new compounds from the marine environment has been gaining attention. From the metagenome library, bioinformatics methods can screen for genes with new biological activities from uncultured microorganisms that is a breakthrough for research and application of biotechnology. In this study, of 6 DNA samples of sponge-associated microorganisms collected in Quang Tri (Vietnam), the sample DNA QT2, with its high content and purity, was selected for metagenomic sequencing (DNA concentration: 202.5 ng, A260/ A280= 1.77). 16S rRNA metagenomic sequencing of QT2 produced 44,117,722 reads, which were assembled into 120,236 contigs. ORF prediction using Prodigal produced 386,416 ORFs. Functional annotation was conducted based on 7 different databases (NR, COG, CAZy, Swissprot, GO, KEGG, Pfam), of which 266,553 genes were annotated using Swiss-Prot. In addition, screening the obtained metagenomic data revealed 50 complete genes encoding protease inhibitor proteins. Of these, 28 genes (>50%) belonged to the serine protease inhibitor family, while the remaining 22 genes belonged to the Inter-alpha-trypsin inhibitor group. NCBI BLAST screening of these proteins showed similarities of more than 50% to protease inhibitors.



Bioinformatics, metagenomics, protease inhibitor, sponge, sponge-associated microorganisms.




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