The Sensitivity of Animal Cells Sensitized With Bilirubin under Irradiation of Blue and Green Spectral Range

O. A. Kozlenkova; L. G. Plavskaya; A. V. Mikulich; I. A. Leusenko; A. I. Tretyakova; V. Yu Plavskii; J. Gao; D. Xiong; X. Wu

doi:10.15625/0868-3166/26/4/9004

The Sensitivity of Animal Cells Sensitized With Bilirubin under Irradiation of Blue and Green Spectral Range

O. A. Kozlenkova, L. G. Plavskaya, A. V. Mikulich, I. A. Leusenko, A. I. Tretyakova, V. Yu Plavskii, J. Gao, D. Xiong, X. Wu

Author affiliations

Authors

O. A. Kozlenkova Laboratory of Organic Heterogeneous Media, B.I. Stepanov Institute of Physics of NAS of Belarus, 68, Nezavisimosti prospect, 220072 Minsk, Republic of Belarus
L. G. Plavskaya Laboratory of Organic Heterogeneous Media, B.I. Stepanov Institute of Physics of NAS of Belarus, 68, Nezavisimosti prospect, 220072 Minsk, Republic of Belarus
A. V. Mikulich Laboratory of Organic Heterogeneous Media, B.I. Stepanov Institute of Physics of NAS of Belarus, 68, Nezavisimosti prospect, 220072 Minsk, Republic of Belarus
I. A. Leusenko Laboratory of Organic Heterogeneous Media, B.I. Stepanov Institute of Physics of NAS of Belarus, 68, Nezavisimosti prospect, 220072 Minsk, Republic of Belarus
A. I. Tretyakova Laboratory of Organic Heterogeneous Media, B.I. Stepanov Institute of Physics of NAS of Belarus, 68, Nezavisimosti prospect, 220072 Minsk, Republic of Belarus
V. Yu Plavskii Laboratory of Organic Heterogeneous Media, B.I. Stepanov Institute of Physics of NAS of Belarus, 68, Nezavisimosti prospect, 220072 Minsk, Republic of Belarus
J. Gao Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, China
D. Xiong Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, China
X. Wu Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, China

DOI:

https://doi.org/10.15625/0868-3166/26/4/9004

Keywords:

hyperbilirubinemia, bilirubin, photosensitizer, cell viability, MTT-test, phototherapy, singlet oxygen

Abstract

It has been shown that exposure to radiation of LED sources of light with an emission band maximum at about 465 and 520 nm having substantially identical damaging effects on animal cells in culture, that are in a logarithmic growth phase and preincubated with pigment. Photobiological effect is caused by photodynamic processes involving singlet oxygen generated by triplet excited sensitizer. Mono-exponential type dependence of cell survival on the energy dose indicates that it is bilirubin that acts as a sensitizer but not its photoproducts. The inclusion of bilirubin in the cells, where it is primarily localized in the mitochondria cells, it is accompanied by multiple amplification photochemical stability compared to pigment molecules bound with albumin.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

07-03-2017

How to Cite

[1]

O. A. Kozlenkova, L. G. Plavskaya, A. V. Mikulich, I. A. Leusenko, A. I. Tretyakova, V. Y. Plavskii, J. Gao, D. Xiong and X. Wu, The Sensitivity of Animal Cells Sensitized With Bilirubin under Irradiation of Blue and Green Spectral Range, Comm. Phys. 26 (2017) 345. DOI: https://doi.org/10.15625/0868-3166/26/4/9004.

Download Citation

Issue

Vol. 26 No. 4 (2016)

Section

Papers

License

Authors who publish with CIP agree with the following terms:

The manuscript is not under consideration for publication elsewhere. When a manuscript is accepted for publication, the author agrees to automatic transfer of the copyright to the editorial office.
The manuscript should not be published elsewhere in any language without the consent of the copyright holders. Authors have the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of their work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to or during the submission process, as it can lead to productive exchanges or/and greater number of citation to the to-be-published work (See The Effect of Open Access).