Research on isolation, identification, and determination of suitable conditions for the cultivation of albino <i>Cordyceps militaris</i> SHBTD fruiting bodies

Dao Ngoc Anh; Truong Thi Chien; Do Thi Kim Trang; Tran Bao Tram; Nguyen Thi Thanh Mai; Thai Hanh Dung; Tran Van Tuan; Vu Xuan Tao

doi:10.15625/vjbt-21015

Author affiliations

Authors

Dao Ngoc Anh \(^1\) Center for Experimental Biology, National Center for Technological Progress, Ministry of Science and Technology, Hanoi, Vietnam https://orcid.org/0009-0000-7317-2282
Truong Thi Chien \(^1\) Center for Experimental Biology, National Center for Technological Progress, Ministry of Science and Technology, Hanoi, Vietnam https://orcid.org/0009-0008-4637-7870
Do Thi Kim Trang \(^1\) Center for Experimental Biology, National Center for Technological Progress, Ministry of Science and Technology, Hanoi, Vietnam https://orcid.org/0009-0006-2105-0068
Tran Bao Tram \(^1\) Center for Experimental Biology, National Center for Technological Progress, Ministry of Science and Technology https://orcid.org/0000-0003-3707-9958
Nguyen Thi Thanh Mai \(^1\) Center for Experimental Biology, National Center for Technological Progress, Ministry of Science and Technology https://orcid.org/0009-0003-2258-5791
Thai Hanh Dung \(^2\) National Key Laboratory of Enzyme and Protein Technology, University of Science, Vietnam National University, Hanoi, Vietnam https://orcid.org/0009-0001-2675-9336
Tran Van Tuan \(^2\) National Key Laboratory of Enzyme and Protein Technology, University of Science, Vietnam National University, Hanoi, Vietnam
\(^3\) Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam https://orcid.org/0000-0003-3916-2624
Vu Xuan Tao \(^1\) Center for Experimental Biology, National Center for Technological Progress, Ministry of Science and Technology, Hanoi, Vietnam https://orcid.org/0009-0007-5320-6554

DOI:

https://doi.org/10.15625/vjbt-21015

Keywords:

Albino, cordycepin, Cordyceps militaris, mating-type (MAT) gene, fruiting body

Abstract

Cordyceps militaris is a valuable fungus that is well-known in many Asian countries. The fruiting bodies of C. militaris are commonly used in traditional medicine and the production of functional foods. Currently, C. militaris mushrooms are being cultivated on a large scale in Vietnam, yielding the characteristic yellow-orange fruiting bodies typical of C. militaris. In the mushroom cultivation industry, C. militaris is one of the most highly valued species. The yield and quality of the fruiting bodies are greatly dependant on the strain source. Therefore, the search for new strains of C. militaris with high productivity, quality, and uniqueness is always of interest to scientists. This study has isolated and identified the albino strain C. militaris SHBTD based on morphological characteristics and the rDNA ITS region gene sequence. The optimal conditions for fruiting body formation of the SHBTD strain are MT6 medium, a temperature of 22°C, a humidity of 90%, a light intensity of 500 lux (12 hours/day), and 65 days of cultivation. Additionally, this study determined that the albino strain C. militaris SHBDT carrying the single Mating-type (MAT) gene (MAT1-1-1), could provide stable yield and quality of fruiting bodies over 5 consecutive cultivation generations. The obtained results demonstrate the potential applications of the C. militaris SHBTD in the industrial-scale production of unique and high-value mushroom products.

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