Biocontrol potentiality of <i>Burkholderia vietnamiensis</i> nrv12 against the rice blast fungus <i>magnaporthe oryzae</i>

Nguyen Thi Thanh Loi; Nguyen Tran Mai Anh; Ho Manh Tuong; Vu Thi Hanh Nguyen; Quach Ngoc Tung; Nguyen Thi Thu An; Do Tien Phat; Chu Hoang Ha; Phi Quyet Tien

doi:10.15625/vjbt-20167

Author affiliations

Authors

Nguyen Thi Thanh Loi \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
\(^2\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam https://orcid.org/0009-0009-1861-491X
Nguyen Tran Mai Anh \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam https://orcid.org/0000-0003-3750-8169
Ho Manh Tuong \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam https://orcid.org/0000-0002-0214-7757
Vu Thi Hanh Nguyen \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
\(^2\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Quach Ngoc Tung \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
\(^2\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam https://orcid.org/0000-0002-1396-9465
Nguyen Thi Thu An \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Do Tien Phat \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
\(^2\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam https://orcid.org/0000-0002-4227-7396
Chu Hoang Ha \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
\(^2\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Phi Quyet Tien \(^1\) Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
\(^2\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam

DOI:

https://doi.org/10.15625/vjbt-20167

Keywords:

Antifungal activity, biocontrol, Burkholderia, chitinase, plant growth promotion, rice blast disease

Abstract

Rice blast disease, caused by the pathogenic fungus Magnaporthe oryzae, is a widespread infection leading to serious crop loss worldwide. In order to achieve sustainable agriculture, root-associated bacteria have been applied to manage fungal diseases and promote growth. The present study aimed to evaluate in vitro the growth-promoting ability and in vivo biocontrol activity against M. oryzae of rice rhizosphere bacterium. Out of sixty-eight isolates recovered from the rhizosphere of blast-infected rice plants, isolate NRV12 exhibited the highest antifungal activity against M. oryzae SH, with an inhibition percentage of 72.7±3.44%. By analysis of 16S rRNA sequence associated with morphology, physiological and biochemical tests, the strain was identified as Burkholderia vietnamiensis. In addition, NRV12 produced hydrolytic enzymes (amylase, cellulase, protease), indole acetic acid (IAA), exhibited nitrogen-fixing potential and the ability to solubilize phosphate and zinc. Innoculation with NRV12 significantly promoted in vivo rice seedling growth to 23.3% as compared to the non-bacteria-treated seedlings. Importantly, infected rice seedlings treated with NRV12 led to a 40% disease reduction in rice blast. These findings suggest that NRV12 is a valuable and promising isolate with biocontrol potential against rice blast caused by M. oryzae.

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