Isolation of cellulose-degrading Cellulosimicrobium from larval gut of Protaetia brevitarsis seulensis

Phan Thi Quy Quyen; Le Thi Van Anh; Ho Le Han; Luan Tran-Van; Tran Thi My Kieu; Tran Thi Ngoc Thu; Nguyen Thi Dong Phuong

doi:10.15625/1811-4989/18201

Author affiliations

Authors

Phan Thi Quy Quyen The University of Da Nang, University of Technology and Education, 48 Cao Thang Street, Hai Chau District, Da Nang City, Vietnam
Le Thi Van Anh Publishing House for Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
Ho Le Han The University of Da Nang, University of Science and Technology, 54 Nguyen Luong Bang Street, Hoa Khanh District, Da Nang City, Vietnam
Luan Tran-Van The University of Da Nang, University of Science and Technology, 54 Nguyen Luong Bang Street, Hoa Khanh District, Da Nang City, Vietnam
Tran Thi My Kieu The University of Da Nang, University of Technology and Education, 48 Cao Thang Street, Hai Chau District, Da Nang City, Vietnam
Tran Thi Ngoc Thu The University of Da Nang, University of Technology and Education, 48 Cao Thang Street, Hai Chau District, Da Nang City, Vietnam
Nguyen Thi Dong Phuong The University of Da Nang, University of Technology and Education, 48 Cao Thang Street, Hai Chau District, Da Nang City, Vietnam

DOI:

https://doi.org/10.15625/1811-4989/18201

Abstract

Larvae live in soil have been well known for containing abundant microbiota in their gut. This study isolated six bacterial genera from the gut of larvae Protaetia brevitarsis seulensis including Bacillus, Cellulosimicrobium, Microbacterium, Streptomyces, Krasilnikoviella and Isoptericola based on specific media. Among these six genera, Cellulosimicrobium was collected for further analysis of cellulose-degrading features because of the most abundance and less studies up to now. Based on the 16S rDNA gene, the Cellulosimicrobium isolates were classified to C. cellulans, C. aquatile, C. funkei, C. protaetiae that were respectively isolated from four specific media such as modified ISP-2, MRS, modified anaerobic medium, modified fermentation medium. The analysis of their genome proved the presence of genes encoding for chitinases, alkyl resorcinol, and glucosidase in four strains. These cellulose degrading enzymes were useful for textile processing, paper recycling, production of nutritional supplements, food industry, production of alcohol from lignocellulosic materials, and beneficial microorganisms in denitrification and N-cycling in forest ecosystem as well as wastewater process.