Investigation of microalgae culture by autoflocculation methodologies

Le Thi Van Anh, Tran Thi Ngoc Thu, Nguyen Thi Dong Phuong
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Authors

  • Le Thi Van Anh Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Tran Thi Ngoc Thu The University of Danang, University of Technology and Education, 48 Cao Thang Street, Hai Chau District, Da Nang City, Vietnam
  • Nguyen Thi Dong Phuong The University of Danang, University of Technology and Education, 48 Cao Thang Street, Hai Chau District, Da Nang City, Vietnam

DOI:

https://doi.org/10.15625/1811-4989/17059

Abstract

Harvesting of microalgae from their different cultivation media has pointed out challenges in resolving the problems of flocculation. These challenges must be faced with a  suitable method for inducing flocculation that avoid or limit the microalgae’s contamination. This study developed the fundamental experiments with a support of chemicals and some bacteria strains inducing the flocculation of Chlorella vulgaris SAG 211-19.  Particularly, the determination of minimum content of Mg2+, Ca2+, E. coli ATCC 85922 and Bacillus subtilis MT300405 was effectuated with co-cultivation of microalgae and set up in batch culture in Bold’s Basal Medium. As a result, the adjustment in 25 minutes of 199.2 mg/L CaCl2.2H2O, 50 mg/L KH2PO4, and of 141 mg/L MgSO4.7H2O induced a microalgal settling efficiency of 81% and 70%, respectively. Meanwhile, the perfomance of microalgal removing reached up to 83.6% and 84% by the inoculation into microalgal culture media of a minimum initial cell density of 8.1 ´ 105 CFU/mL of Bacillus subtilis MT300405 and 12 ´ 105 CFU/mL of E. coli ATCC 85922, respectively. The flocculation of microalgal cells by bacterial inoculation did not require a high pH adjustment as in the case of salt addition.

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Published

30-09-2022

How to Cite

Van Anh, L. T., Ngoc Thu, T. T., & Thi Dong Phuong, N. (2022). Investigation of microalgae culture by autoflocculation methodologies. Vietnam Journal of Biotechnology, 20(3), 487–494. https://doi.org/10.15625/1811-4989/17059

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