Evaluating photosynthetic performance of transgenic soybean harboring CKX13 under salinity conditions

Vo Thi My Duyen, Hoang Thi Lan Xuan, Tran Nguyen Hoang Tu, Nguyen Tien-Dung, Nguyen Phuong Thao
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Authors

  • Vo Thi My Duyen Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam
  • Hoang Thi Lan Xuan Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam
  • Tran Nguyen Hoang Tu Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam
  • Nguyen Tien-Dung College of Agriculture and Forestry, Thai Nguyen University, Quyet Thang Commune, Thai Nguyen City, Vietnam
  • Nguyen Phuong Thao Applied Biotechnology for Crop Development Research Unit, School of Biotechnology, International University, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

DOI:

https://doi.org/10.15625/1811-4989/17661

Abstract

Cytokinins (CKs) are considered one of the critical phytohormones with an important role in plant response to abiotic stresses. One of the key enzymes in regulating CK levels is CK oxidase/dehydrogenase (CKX), which catalyzes the irreversible CK degradation. Although CKX gene members in soybean (Glycine max) have been identified, their importance in plant tolerance against salinity has not yet been fully elucidated. In this study, GmCKX13, a gene with upregulated expression in various tissues of soybean at various stages under water deficit condition as shown in previous investigation, was selected for exploring its role in relation to photosynthetic performance under salt stress conditions. The results showed that the transgenic soybean overexpressing GmCKX13 could maintain a better assimilation rate compared to their wild-type (WT) counterparts under the salinity condition. This could be explained by the higher chlorophyll content and the less damage caused by the stress in the photosynthetic machinery of the transgenic plants. According to the analyses, the transformers displayed higher values of maximal photosystem II (PSII) photochemical efficiency (Fv/Fm), maximal quantum yield of primary PSII photochemistry (φPo), quantum yield for electron transport (φEo) and efficiency for electron being transferred from QA- to plastoquinone (ψEo). The evaluation for the overall photosynthetic performance using performance index in absorption basis (PIABS) also supported the differential photosynthesis potential between the two genotypes. Collectively, these findings suggested that appropriate GmCKX13 modulation could enhance photosynthetic adaptation to salt-stressed conditions in soybeans.

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Published

30-03-2023

How to Cite

My Duyen, V. T., Lan Xuan, H. T., Hoang Tu, T. N., Tien-Dung, N., & Phuong Thao, N. (2023). Evaluating photosynthetic performance of transgenic soybean harboring CKX13 under salinity conditions. Vietnam Journal of Biotechnology, 21(1), 83–92. https://doi.org/10.15625/1811-4989/17661

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