Investigation of anti-allergic activity of piceatannol from Rhodomyrtus tomentosa fruits


  • Vo Thanh Sang
  • Nguyen Hoang Nhat Minh
  • Ngo Xuan Quang Institute of Tropical Biology, Vietnam Academy of Science and Technology
  • Pham Ngoc Hoai
  • Bach Long Giang
  • Le Van Minh
  • Nguyen Huu Hung
  • Nguyen Luong Hieu Hoa
  • Ngo Dai Hung



Allergy, degranulation, mast cells, Rhodomyrtus tomentosa, piceatannol


Piceatannol is a naturally occurring polyphenolic stilbene found in various fruits and vegetables. It has been reported to possess various pharmaceutical properties and health benefit effects such as anticancer, antidiabetes, antioxidant, anti-inflammation, and anti-obesity activities. Recently, Rhodomyrtus tomentosa fruits have been determined to contain a large amout of piceatannol. In this study, piceatannol was extracted and isolated from R. tomentosa fruits collected from Phu Quoc district. Moreover, the anti-allergic activity of piceatannol was investigated using RBL-2H3 cells as an in vitro experimental model. Firstly, piceatannol was isolated from ethyl acetate extract of R. tomentosa fruit powder via using a column chromatography and high performance liquid chromatography eluted by several types of binary solvent systems with different polarity. Subsequently, the characterization of the isolated compound was identified by using the mass spectrometer and nuclear magnetic resonance methods. The degranulation of mast cellls was examined via measuring the release of β-hexsosaminidase in the culture supernatant. Moreover, observation of cell morphology was conducted under the inverted microscope as the RBL-2H3 cells treated with 40 µM of piceatannol. In addition, the cytotoxic effect of piceatannol at the concentration treatment of 40 µM was also tested via MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. As the result, piceatannol treatment significantly inhibited the mast cell degranulation via reducing the β-hexsosaminidase release to 44% at the concentration of 40 µM. Moreover, piceatannol exhibited the protective effect against calcium ionophore-induced morphological change of the RBL-2H3 cells. Notably, no significant cytotoxic effect at the concentration treatment up to 40 µM of piceatannol was observed on RBL-2H3 cells. Therefore, piceatannol from the R. tomentosa fruits could be suggested as a potential anti-allergic agent for future therapeutics.


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Author Biography

Ngo Xuan Quang, Institute of Tropical Biology, Vietnam Academy of Science and Technology

  1. nematology,
  2. aquatic ecology,
  3. aquatic environment,
  4. invertebrate,
  5. bioindicator,
  6. estuarine ecology,
  7. marine biology,
  8. communities ecology,
  9. ecotoxicology


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How to Cite

Sang, V. T., Nhat Minh, N. H., Quang, N. X., Hoai, P. N., Long Giang, B., Minh, L. V., Huu Hung, N., Hieu Hoa, N. L., & Dai Hung, N. (2021). Investigation of anti-allergic activity of piceatannol from Rhodomyrtus tomentosa fruits. Vietnam Journal of Biotechnology, 19(1), 119–128.