Genetic variation of Anisoptera costata Korth in Tan Phu tropical forest, Dinh Quan district, Dong Nai province

Dang Phan Hien, Nguyen Minh Duc, Nguyen Phan Lan Hong, Bui Thi Tuyet Xuan, Vu Dinh Duy, Nguyen Minh Tam
Author affiliations


  • Dang Phan Hien
  • Nguyen Minh Duc
  • Nguyen Phan Lan Hong
  • Bui Thi Tuyet Xuan
  • Vu Dinh Duy
  • Nguyen Minh Tam Bảo tàng Thiên nhiên Việt Nam



Bảo tồn, Đa dạng di truyền, microsatellite, Phân cắt nơi sống, Vên vên


Anisoptera costata Korth, an endangered species is distributed in lowland tropical forests of southern Vietnam. Habitat loss and over-exploitation are the major reasons for threatening this species. Eight polymorphic microsatellite markers were used to analyze 64 adult trees from three A. costata populations in lowland tropical forests of Tan Phu, Dinh Quan district, Dong Nai Province in Southeast Vietnam to detect the effects of deforestation on gene flow and the differentiation among populations in lowland tropical forests. The results showed that all A. costata populations have the moderate levels of the genetic diversity within populations with mean values of observed and expected heterozygosities, 0.242 and 0.269, respectively, moderate genetic differentiation among A. costata populations (0.179), and indicating limited gene flow (Nm = 1.15). Analysis of molecular variance indicated high genetic variation within populations (64.68%) and indicating moderate genetic structure in A. costata in Tan Phu. Bayesian analysis detected two genetic lineages, cluster 1 including one population of Mieu Co Nam and cluster 2 including two populations, Thac Mai and Bau Nuoc. These results contribute understanding genetic diversity of A. costata in lowland forests of Southeastern Vietnam and will provide guidelines for conservation, management and resoration of the species.


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Abasolo MA, Fernando ES, Borromeo TH, Hautea DM (2009) Cross-species amplification of Shorea microsatellite DNA markers in Parashorea malaanonan (Dipterocarpaceae). Philippine J Sci 138:23-28.

BKHCN (2007) Sách đỏ Việt Nam. Nhà XBKHCN, Hà Nội.

Chybicki IJ, Burczyk J (2009) Simultaneous estimation of null alleles and inbreeding coefficient. J Hered 100:106-113.

Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small qualities of fresh leaf tissue. Phytochem Bull 19:11-15.

Duc NM, Duy VD, Xuan BTT, Thang BV, Ha NTH, Tam NM (2016) Genetic structure in threatened Dipterocarpus costatus populations in lowland tropical rainforests of southern Vietnam. Genet Mole Res 15 (4).

Earl DA, von-Holdt BM (2012) Structure Harvester: a website and program for visualizing structure output and implementing the Evanno method. Cons Genet Res 4:359-61.

Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14:2611-20.

Excoffier L, Laval G, Schneider S (2005) Arlequin v. 3.0. an integrated software package for population genetics data analysis. Evol Bioinformatics online 1:47-50.

Goudet J (2003) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.4). Available from

Finkeldey R, Hattemer HH (2007) Tropical forest genetics. Springer-Verlag, Berlin, Heidelber. doi: 10.1007/978-3-540-37398-8.

Isagi V, Kenta T, Nakashizuka T (2002) Microsatellite loci for a tropical emergent tree, Dipterocarpus tempehes V. S1 (Dipterocarpaceae). Mol Ecol Notes 2:12-13.

Harada K, Dwiyanti FG, Siregar IS, Subiakto A, Chong L, Diway B, Lee YF, Ninomiya I, Kamiya K (2018) Genetic variation and genetic structure of two closely related dipterocarp species, Dryobalanops aromatica C.F.Gaertn. and D. Beccarii Dyer. J Bot Gard Hort 16: 179-187.

Kalnowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16(5): 1099-1106. doi:10.1111/j.1365-294X.2007.03089.x

Ng KSK, Lee SL, Koh CL (2004) Spatial structure and genetic diversity of two tropical tree species with contrasting breeding systems and different ploidy levels. Mol Ecol 1-13. http://doi:10.1046/j.1365-294X.2004.0294.x.

Nghia NH (2005) Dipterocarps of Vietnam. Agri. Hou. Hanoi.

Nguyen HN, Vu VD, Luu HT, Hoang VS, Pooma R, Khou E, Nanthavong K, NEWMAN MF, Ly V (2017) Barstow. Anisoptera costata. The IUCN Red List of Threatened Species.e.T33166A2833752.

Pandey M, Geburek T (2009) Successful cross-amplification of Shorea microsatellites reveals genetic variation in the tropical tree, Shorea robusta Gaertn. Hereditas 146:29-32.

Peakall R, Smouse PE (2012) GenAlex 6.5: genetic analysis in excel. Population genetic software for teaching and research an update. Bioinformatics 28:2537-39.

Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945-59.

Rachmat HH, Kamiya K, Harada K (2012) Genetic diversity, population structure and conservation implication of the endemic Sumatran lowland dipterocarp tree species (Shorea javanica. Int J Biodiv Cons 4:573-583. doi: 10.5897/IJBC12.045.

Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Res 8:103–106.

Terauchi R (1994) A polymorphic microsatellite marker from the tropical tree Dryobalanops lanceolata (Dipterocarpaceae). Japan J Genet 69:567-76.

Trang NTP, Huong TT, Duc NM, Tim S, Triest L (2014) Genetic population of threatened Hopea odorata Roxb. in the protected areas of Vietnam. J Vietn Env 6:69-76. doi: 10.13141/jve.

Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley DF (2004) Micro-Cheker: software for identifying and correcting genotyping errors in microsatellile data. Mol Ecol Notes 4: 435-538. .

Vu DD, Bui TTX, Nguyen MD, Shah SNM, Vu DG, Zhang Y, Nguyen MT and Huang XH (2019) Genetic diversity and conservation of two threatened dipterocarps (Dipterocarpaceae) in southeast Vietnam. J For Res 30(5): 1823–1831.

Ujino T, Kawaharam T, Tsumara Y, Nagamitsu T, Yoshimaru H, Ratnam W (1998) Development and polymorphism of simple sequence repeat DNA markers for Shorea curtisii and other Dipterocarpaceae species. Heredity 81:422-28.




How to Cite

Hien, D. P., Duc, N. M., Hong, N. P. L., Xuan, B. T. T., Duy, V. D., & Tam, N. M. (2021). Genetic variation of Anisoptera costata Korth in Tan Phu tropical forest, Dinh Quan district, Dong Nai province. Vietnam Journal of Biotechnology, 19(2), 279–288.




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