High-frequency in vitro shoot regeneration of Saintpaulia ionantha Wendl. by light-emitting diodes

Le The Bien, Hoang Thanh Tung, Hoang Dac Khai, Do Manh Cuong, Vu Quoc Luan, Nguyen Ba Nam, Trinh Thi Huong, Bui Van The Vinh, Duong Tan Nhut
Author affiliations

Authors

  • Le The Bien
  • Hoang Thanh Tung Tay Nguyen Institute for Scientific Research, VAST https://orcid.org/0000-0002-2614-0429
  • Hoang Dac Khai
  • Do Manh Cuong
  • Vu Quoc Luan
  • Nguyen Ba Nam
  • Trinh Thi Huong
  • Bui Van The Vinh
  • Duong Tan Nhut

DOI:

https://doi.org/10.15625/1811-4989/13776

Keywords:

Tử linh lan, nhân chồi, lá, đèn LED

Abstract

Saintpaulia ionantha Wendl. is a small flower, with a variety of colors, which are commonly grown indoors for decorative purposes. Currently, micropropagation of S. ionantha has been achieved certain results; however, the shoot quality as well as number of shoots regeneration was not high. In this study, the effect of LEDs system on the shoot multiplication and shoot quality of S. ionantha was conducted. The leaves were cut into square patterns without the main veins (1 cm × 1 cm), and the petiole (3 mm in diameter) was cut with a thick layer (2 mm) of longitudinal cell (lTCL), which were used as the initial materials. The explants were placed under LEDs conditions; and white fluorescent tube lamp (FL) was used as the control for shoot regeneration and rooting. The results found that the quality of light affects the regeneration, growth and development of S. ionantha shoots. The LED condition with a combination of 7R + 3B are optimal for high-frequency of shoot regeneration with the regeneration rate: 67.25%, and number of shoots: 9.33 shoots/explant from the leaves without the main veins. The higher shoot multiplication (number of shoots: 10.00 shoots/explant, fresh weight: 150.07 mg, dry weight: 98.64 mg) and rooting rate of plantlet (fresh weight: 2.17 g; dry weight: 0.09 g; leaf number: 20.33 leaves/plantlet) were also obtained under 7R + 3B condition.

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Published

30-12-2021

How to Cite

Bien, L. T., Thanh Tung, H., Dac Khai, H., Manh Cuong, D., Luan, V. Q., Ba Nam, N., Thi Huong, T., The Vinh, B. V., & Nhut, D. T. (2021). High-frequency in vitro shoot regeneration of Saintpaulia ionantha Wendl. by light-emitting diodes . Vietnam Journal of Biotechnology, 19(4), 717–724. https://doi.org/10.15625/1811-4989/13776

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