. In vitro propagation of the new orchid Dendrobium trankimianum T. Yukawa

H’ Yon Nie Bing, Dang Thi Tham, Tran Thai Vinh, Quach Van Hoi, Vu Kim Cong, Nong Van Duy
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Authors

  • H’ Yon Nie Bing
  • Dang Thi Tham
  • Tran Thai Vinh
  • Quach Van Hoi
  • Vu Kim Cong
  • Nong Van Duy Tay Nguyen Institute of Scientific resarch

DOI:

https://doi.org/10.15625/1811-4989/16/4/11155

Keywords:

Conservation, Dendrobium trankimianum T. Yukawa, in vitro, substrate, PLBs, wild orchids.

Abstract

Dendrobium trankimianum T. Yukawa is a beautiful, endemic orchid of Vietnam, a new species with a first - published description in 2004. It is very rare and expected to be added to the IUCN Red List status - CR. In vitro studies of orchid D. trankimianum T. Yukawa were conducted in order to conserve and increase the genetic pool of this precious wild orchid species. The results showed that full-strength MS medium supplemented with 2.0 mg/L BA and 0.5 mg/L NAA (10.24 PLBs/explant; 90.11% explants formed PLBs) or full-strength MS medium supplemented with 1.5 mg/L TDZ and 0.5 mg/L NAA (14.11 PLBs/explant; 92.06% explants formed PLBs) were the most suitable for protocorm formation. For subculture, suitable growth of shoots were obtained on full-strength MS medium supplemented 1.5 mg/L BA (22.35 shoots/explant; shoots length of 1.96 cm) and full-strength MS medium supplemented with 60 g ripe banana per liter (25.11 shoots/explant; shoots length of 2.12 cm). The shoots in vitro were transferred to half-strength MS supplemented with different concentrations of IAA, IBA and NAA to investigate root formation. The best rooting occurred at 0,5 mg/L NAA (7.91 roots/shoot; root length of 4.01 cm; 98.51% root formation). The plantlets with uniform growth were planted on different substrate: Eco clean soil, Coconut fiber, Fern fiber, 50% Rice husk in combination with 50% Eco clean soil for research the most suitable substrate. After 60 days of transplantion and acclimatization, the result showed that Fern fiber was suitable substrate for plantlet growth in a nursery garden (8.0 roots/ explant; root length of 5.5 cm; survival rate of 93.29%).

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Published

08-08-2020

How to Cite

Yon Nie Bing, H., Thi Tham, D., Thai Vinh, T., Van Hoi, Q., Kim Cong, V., & Duy, N. V. (2020). . In vitro propagation of the new orchid Dendrobium trankimianum T. Yukawa. Vietnam Journal of Biotechnology, 16(4), 649–657. https://doi.org/10.15625/1811-4989/16/4/11155

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