Insight into Paphiopedilum x Dalatense Aver. (Orchidaceae) origin based on morphological and molecular markers

Tran Thai Vinh, H’ Yon Nie Bing, Vu Kim Cong, Dang Thi Tham, Ngo Phuong Linh, Le Ngoc Trieu, Nong Van Duy
Author affiliations

Authors

  • Tran Thai Vinh Tay Nguyen Institute of Scientific Research, Vietnam Academy of Science and Technology, 116 Xo Viet Nghe Tinh Road, Da Lat City, Lamdong Province, Vietnam
  • H’ Yon Nie Bing Tay Nguyen Institute of Scientific Research, Vietnam Academy of Science and Technology, 116 Xo Viet Nghe Tinh Road, Da Lat City, Lamdong Province, Vietnam
  • Vu Kim Cong Tay Nguyen Institute of Scientific Research, Vietnam Academy of Science and Technology, 116 Xo Viet Nghe Tinh Road, Da Lat City, Lamdong Province, Vietnam
  • Dang Thi Tham Tay Nguyen Institute of Scientific Research, Vietnam Academy of Science and Technology, 116 Xo Viet Nghe Tinh Road, Da Lat City, Lamdong Province, Vietnam
  • Ngo Phuong Linh Da Lat University, 1 Phu Dong Thien Vuong Street, Da Lat City, Lamdong Province, Vietnam
  • Le Ngoc Trieu Da Lat University, 1 Phu Dong Thien Vuong Street, Da Lat City, Lamdong Province, Vietnam
  • Nong Van Duy Tay Nguyen Institute of Scientific Research, Vietnam Academy of Science and Technology, 116 Xo Viet Nghe Tinh Road, Da Lat City, Lamdong Province, Vietnam

DOI:

https://doi.org/10.15625/1811-4989/15909

Keywords:

ITS1-5.8S rRNA-ITS2, hybrid species, Paphiopedilum × dalatense, partial matK gene, reversible.

Abstract

Paphiopedilum callosum, Paphiopedilum villosum and Paphiopedilum ´ dalatense are endangered species and are currently listed as Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES, 2019) Appendix I species. Thus, the international trade for these naturally occurring species is forbidden. However, the in vitro propagated materials and their inter-species hybrids forced by breeders can be traded for commercial purposes. P. ´ dalatense is an endemic slipper orchid species of Vietnam with high aesthetic value. Currently, the natural reserves of this species have been greatly reduced by overexploitation. In this study, P. ´ dalatense was demonstrated to be the reversible hybrid species between P. callosum and P. villosum based on both morphological and molecular markers. P. ´ dalatense possesses intermediate morphological characteristics between P. callosum and P. villosum which could be recorded in leaf, in flower and its components such as lip, petal, and dorsal sepal. There were 23 sites in ITS1-5.8S rRNA-ITS2 region sequence of P. ´ dalatense which possess the nucleotide characteristic of both P. callosum and P. villosum. There were two out of three P. ´ dalatense investigated samples possessed partial matK gene which was identical to the corresponding sequence in P. villosum and the last sample possessed identical partial matK gene to P. callosum. Proving P. ´ dalatense to be the reversible hybrid species between P. callosum and P. villosum is the essentially scientific base for commercial breeding of P. ´ dalatense by artificial hybridization which contributes to avoid overexploiting the natural P. ´ dalatense resources.

Downloads

Download data is not yet available.

References

Averyanov L, Cribb PJ, Loc PK, Hiep NT (2003) Slipper orchid of Vietnam. With an Introduction to the Flora of Vietnam. Royal Botanic Gardens, Kew; UK ed. Edition.

Averyanov L (2001) New natural interspecific hybrid - Paphiopedilum  dalatense from Vietnam. Orchid Digest 65(3): 133-134.

Chochai A, Leitch IJ, Ingrouille MJ, Fay MF (2012) Molecular phylogenetics of Paphiopedilum (Cypripedioideae; Orchidaceae) based on nuclear ribosomal ITS and plastid sequences. Bot J Linn Soc 170: 176-196.

Corriveau JL, Coleman AW (1988) Rapid screening method to detect potential biparental inheritance of plastid DNA and results for over 200 angiosperm species. Amer J. Bot. 75(10): 1443-1458.

Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11-15.

Goodstadt L, Ponting CP (2001) CHROMA: consensus-based colouring of multiple lignments for publication. Bioinformatics 17(9): 845-46.

Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Paper presented at the Nucl. Acids Sympos. Ser.

Klein RM, Klein DT (1970) Research Methods in Plant science. Garden City, Newyork. Natural History Press.

Moller M, Cronk QC (1997) Origin and relationships of Saintpaulia (Gesneriaceae) based on ribosomal DNA internal transcribed spacer (ITS) sequences. Amer J. Bot. 84(7): 956-965.

Thin NN (2007) Methods for plant research. Hanoi National University Publisher.

Ban NT (2007) Vietnam Red Book - Plant. Natural Science and Technology Publishing.

Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain - terminating inhibitors. Proc Nalt Acad Sci 74(12): 5463-5467.

Szlachetko DL, Kolanowska M, Muller F, Vannini J, Rojek J, Górniak M (2017) First Guatemalan record of natural hybridisation between Neotropical species of the Lady’s Slipper orchid (Orchidaceae, Cypripedioideae). PeerJ 5: e4162.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12): 2725-2729.

Weising K, Nybom H, Wolff K, Kahl G (2005) DNA fingerprinting in plants: principles, methods, and applications. CRC press.

Zuo Y, Chen Z, Kondo K, Funamoto T, Wen J, Zhou S (2011) DNA barcoding of Panax species. Planta Med 77(02): 182-187.

Downloads

Published

30-06-2022

How to Cite

Thai Vinh, T., Nie Bing, H. Y., Kim Cong, V., Tham, D. T., Phuong Linh, N., Ngoc Trieu, L., & Duy, N. V. (2022). Insight into Paphiopedilum x Dalatense Aver. (Orchidaceae) origin based on morphological and molecular markers. Vietnam Journal of Biotechnology, 20(2), 279–287. https://doi.org/10.15625/1811-4989/15909

Issue

Section

Articles

Most read articles by the same author(s)