Using the RAPD-PCR technique to indentify the genetic ralationship of some Calothrix species isolated from cultural soil of the Dak Lak province
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DOI:
https://doi.org/10.15625/0866-7160/v28n1.835Abstract
Five pure species of the genus Calothrix have been isolated from soil samples in the Daclac province and classified by the classical method. We have used the RAPD-PCR technique to explore the genetic relationship of these 5 Calothrix species. The results showed that 75 bands were multiplied by 3 random primers OPA4, OPA10 and OPL12; each of which on average had 25 bands for these Calothrix species. There were 74 polymorphic bands and one band was common marker for these species. The RAPD-PCR technique showed the genetic difference among these 5 species. The findings by using the RAPD-PCR technique were consistent with the results of classical methods.