Detection of single nucleotide polymorphisms (SNPs) in a SARS-CoV-2 virus strain in Vietnam

Tran Xuan Thach; Bui Thi Thuy Duong; Le Van Truong; Nguyen Thi Hoa; Pham Thi Lanh; Nguyen Thai Uy; Le Quynh Mai; Dang Duc Anh; Dinh Duy Khang; Dong Van Quyen

doi:10.15625/2615-9023/15846

Author affiliations

Authors

Tran Xuan Thach Institute of Biotechnology, VAST, Vietnam
Bui Thi Thuy Duong Institute of Biotechnology, VAST, Vietnam https://orcid.org/0000-0003-1002-7517
Le Van Truong Institute of Biotechnology, VAST, Vietnam
Nguyen Thi Hoa Institute of Biotechnology, VAST, Vietnam
Pham Thi Lanh Institute of Biotechnology, VAST, Vietnam
Nguyen Thai Uy Institute of Biotechnology, VAST, Vietnam
Le Quynh Mai The National Institute of Hygiene and Epidemiology, Ha Noi, Vietnam
Dang Duc Anh
Dinh Duy Khang Institute of Biotechnology, VAST, Vietnam
Dong Van Quyen Institute of Biotechnology, VAST, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/15846

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of a pandemic of acute respiratory disease in humans. This pandemic has now spread worldwide and caused more than 2,000,000 deaths by 15 January 2021. The complexity and the ongoing pandemic of coronavirus SARS-CoV-2 make it difficult to control the disease. Sequence analysis on some open reading frames (ORFs) of a SARS-CoV-2 virus strain isolated in Vietnam in February 2020 (SARS-CoV-2/NIHE/human/2020/VIE strain) revealed some single nucleotide polymorphisms (SNPs) that only appeared in the Vietnamese strain in comparison to those of the isolates in other countries, including: (1) a change in ORF8-Nucleocapsid (accession number MT127114.1) at nucleotide (nt) 691 (CTC in SARS-CoV-2/NIHE/human/2020/VIE strain, TTC in all other isolates) but it does not change the encoded amino acid, (2) ORF3a-EM-ORF6-ORF7a region (accession number MT127115.1) has four-point changes, three of which lead to changes in the amino acid sequences, being nt 479 (GTA encoding Valine changed into TTA encoding Leucine), nt 575 (CGC encoding Arginine changed to GGC encoding Glycine) in the M gene, and nt 1126 (GTG encoding Valine changed to GAG encoding Glutamic acid) in ORF6. Taken together, the results provided useful information for the SARS-CoV-2 diagnostic kit and vaccine development.

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