High level expression of a gene coding for pectate lyase in Bacillus subtilis 168

Authors

  • Vo Hoai Bac VAST
  • Do Thi Thu Hang VAST
  • Le Trong Tai VAST
  • Le Van Truong VAST

DOI:

https://doi.org/10.15625/0866-7160/v35n3.3393

Keywords:

Bacillus, expression, pectate lyase, promoter

Abstract

Pectate lyase degrade polygalacturonate of plant cell wall productes oligogalacturonates. In recent years, pectate lyase has attracted the considerable research interest because of their potential industrial application, for instance in the textile industry, paper making. Gene pel coding for pectate lyase from Bacillus subtilis VBS11 was subcloned and fused with α-amylase promoter from B. amyloliquefaciens (BApro). This construct was ligated into vector pMSE3 and transformated into the host strain BS168 for over expression under control of BApro promoter from B. amyloliquefaciens. rPel was producted in high level of 88,6 U/ml in Belisky minimal medium.

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Published

16-10-2013

How to Cite

Bac, V. H., Thu Hang, D. T., Tai, L. T., & Truong, L. V. (2013). High level expression of a gene coding for pectate lyase in Bacillus subtilis 168. Academia Journal of Biology, 35(3), 369–375. https://doi.org/10.15625/0866-7160/v35n3.3393

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