Antimicrobial and antioxidant activity of bacterial endophytes isolated from leaves of the mangrove plant Rhizophora stylosa
Author affiliations
DOI:
https://doi.org/10.15625/0866-7160/v41n4.14675Keywords:
Rhizophora stylosa, antimicrobial activity, antioxidant activity, endophytic bacteria, mangroves.Abstract
Mangroves are the most productive ecosystems and contain highly diverse plants and microbial communities. Mangrove endophytes are proved to be a rich source of bioactive secondary metabolites. The biological molecules produced by endophytes play an important role in protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate the endophytic bacteria from the mangrove plant Rhizophora stylosa and screen antimicrobial and antioxidant activity of ethyl acetate extracts from the isolated endophytic bacteria. A total of 64 endophytic bacterial strains from R. stylosa leaves were isolated, of which ethyl acetate extracts of 14 isolated endophytic strains showed antimicrobial activity against at least one of reference microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 25923, Bacillus subtilis ATCC 27212, Staphylococcus aureus ATCC 12222 and Candida albicans ATCC 7754 with MIC values from 32 to 512 µg/ml. Among them, four strains showed activity against one reference microorganism, five strains showed activity against two reference microorganisms, four strains showed activity against three reference microorganisms, and one strain showed activity against four reference microorganisms. Additionally, the ethyl acetate extracts of 12 isolated endophytic bacteria showed ATBS and DPPH radical scavenging activity with scavenging values from 36.27 ± 2.6% to 71.46 ± 6.6% and from 26.22 ± 3.3% to 57.38 ± 5.8%, respectively. The identification of the five most active endophytic bacteria by 16S rRNA sequences revealed that the endophytes belonged to four genera, including Bacillus, Streptomyces, Pseudovibrio and Pseudomonas. The obtained results suggest that the endophytic bacteria from mangrove plants are a promising reservoir of antimicrobial and antioxidant agents.
Downloads
Metrics
References
Alvin A., Miller K. I., Neilan B. A., 2014. Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds. Microbiological Research, 169: 483–495.
Ancheeva E., Daletos G., Proksch P., 2018. Lead compounds from mangrove-associated microorganisms. Marine drugs, 16: 319.
Carroll G., 1988. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology, 69: 2–9.
Dat T. T. H., Hong T. T., Dung T. T. K, Hoa N. P., Cuong P. V., Binh P. T., Dat N. T., Cuc N. T. K., 2018. Screening of antimicrobial producing bacteria associated with sponge and isolation of secondary metabolites from selected strain. Proceedings of National Conference on Biotechnology 2018. Publishing House of Natural Science and Technology, Ha Noi, Vietnam,
pp. 774–779.
Dat T. T. H., Oanh P. T. T., Tam V. T. T., Anh H. L. T., 2019. Antimicrobial activity of bacteria isolated from the coastal mangrove sediment in Thua Thien Hue. Proceedings of National Scientific Forum 2019: Marine Biology and Sustainable Development. Publishing House of Natural Science and Technology, Hai Phong, Vietnam, pp. 971–980.
Eldeen I., 2014. Isolation of 12 bacterial endophytes from some mangrove plants and determination of antimicrobial properties of the isolates and the plant extracts. International Journal of Phytomedicine, 6: 425–432.
Fareza M. S., Choironi N. A., Harwoko H., Sunarto S., 2018. Antibacterial activity of two isolated endophytic fungi extracts associated with Indonesian mangrove plant Rhizophora mucronata. Pharmaciana, 8: 169–175.
Glick B. R., Penrose D. M., Li J., 1998. A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. Journal of Theoretical Biology, 190: 63–68.
Handayani D., Rivai H., Hutabarat M., Rasyid R., 2017. Antibacterial activity of endophytic fungi isolated from mangrove plant Sonneratia griffithii Kurz. Journal of Applied Pharmaceutical Science, 7: 209–212.
Hoai P. T. T., Dat T. T. H., Hong T. T., Cuc N. T. K., Man T. D., Cuong P. V., 2018. Isolation and selection of indigenous antifungal microorganisms against pathogenic fungi of pepper plant in Tay Nguyen. Vietnam Journal of Biotechnology, 16: 385–392.
Hong H. T., Phuong N. N., 2013. The isolation and selection of actinomycete species from Can Gio tropical swamp for their antifungal feature against Fusarium sp. Journal of Science, Ho Chi Minh City University of Education, 51: 59–71.
Kui-Wu W., Shi-Wei W., Bin W0, Ji-Guang W., 2014. Bioactive natural compounds from the mangrove endophytic fungi. Mini-Reviews in Medicinal Chemistry, 14: 370–391.
Manganyi M. C., Tchatchouang C. D. K., Regnier T., Bezuidenhout C. C., Ateba C. N., 2019. Bioactive compound produced by endophytic fungi isolated from Pelargonium sidoides against selected bacteria of clinical importance. Mycobiology, 47: 335–339.
Nia R., Mia M., Oktapiana K., 2017. Antibacterial activity test of endophytic fungus from mangrove plant (Rhizophora apiculata L.) and (Bruguiera gymnorrizha (L.) Lamk.) against Klebsiella pneumoniae ATCC 700603. ICSAFS Conference Proceedings of 2nd International Conference on Sustainable Agriculture and Food Security: A Comprehensive Approach. KnE Life Sciences, West Java, Indonesia, pp. 146–157.
O'Sullivan D. J., O'Gara F., 1992. Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens. Microbiological Reviews, 56: 662–676.
Rahmawati S. I., Izzati F. N., Hapsari Y., Septiana E., Rachman F., Bustanussalam, Simanjuntak P., 2019. Endophytic microbes and antioxidant activities of secondary metabolites from mangroves Avicennia marina and Xylocarpus granatum. IOP Conference Series: Earth and Environmental Science, 278: 012065.
Richardson A. E., Barea J. M., McNeill A. M., Prigent-Combaret C., 2009. Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant and Soil, 321: 305–339.
Ryan R. P., Germaine K., Franks A., Ryan D. J., Dowling D. N., 2008. Bacterial endophytes: recent developments and applications. FEMS Microbiology Letters, 278: 1–9.
Sivaramakrishnan S., Gangadharan D., Nampoothiri K., Soccol C., Pandey A., 2006. α-amylases from microbial sources–an overview on recent developments. Food Technology and Biotechnology, 44: 173–184.
Stone J., Bacon C., White J., 2000. An overview of endophytic microbes: endophytism defined. In: Bacon CW & White J (Eds) Microbial endophytes, CRC Press, Boca Raton, Florida, USA, pp. 3–29.
Thatoi H., Behera B. C., Mishra R. R., Dutta S. K., 2013. Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: a review. Annals of Microbiology, 63: 1–19.
Tudzynski B., 1997. Fungal phytohormones in pathogenic and mutualistic associations. In: Carroll GC & Tudzynski P (Eds) Plant Relationships: Part A, Springer Berlin Heidelberg, Berlin, Heidelberg, Germany, pp. 167–184.
Xu J., 2015. Bioactive natural products derived from mangrove-associated microbes. RSC Advances, 5: 841–892.
Zhou J., Diao X., Wang T., Chen G., Lin Q., Yang X., Xu J., 2018. Phylogenetic diversity and antioxidant activities of culturable fungal endophytes associated with the mangrove species Rhizophora stylosa and R. mucronata in the South China Sea. PloS one, 13: e0197359.
Downloads
Published
How to Cite
Issue
Section
