Research on application of bromelain and alcalase for production of hydrolyzed powder from round scads
(Decaterus punctatus)
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DOI:
https://doi.org/10.15625/1859-3097/20795Keywords:
Hydrolyzed powder, round scad, Decaterus punctatus, enzyme technology.Abstract
Round scad (Decaterus punctatus) is a species with high nutritional value and is used mainly as food for humans, and partly as food for aquatic animals. There are no processed products from scad fish in our country; thus, the value of scad fish products still needs to be higher. In this report, we have established the hydrolysis procedure by the enzyme mixture of bromelain and alcalase with the following conditions: the enzyme alcalase/bromelain ratio of 1/3; the enzyme and substrate ratio of 1%; hydrolysis temperature at 50oC; nature pH of fish; the incubated time of 6 h; the speed of 200 rpm; The hydrolyzed powder product is rich in protein (74.5%), and minerals (calcium, potassium, phosphorus, copper), thus has many potential applications in functional foods.
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Ministry of Health, Institute of Nutrition, 2007. Vietnamese food composition table. Medical Publishing House, 527 p. (in Vietnamese).
He, C., Sun, Z., Qu, X., Cao, J., Shen, X., and Li, C., 2020. A comprehensive study of lipid profiles of round scad (Decapterus maruadsi) based on lipidomic with UPLC-Q-Exactive Orbitrap-MS. Food Research International, 133, 109138. DOI: https://doi.org/10.1016/j.foodres.2020.109138
Gebhardt, S. E., Lemar, L. E., Cutrufelli, R. L., Haytowitz, D. B., Howe, J. C., Pehrsson, P. R., Stup, M. A., Exler, J., Holcomb, G. T., Thomas, R. G., Showell, B. A., and Holden, J. M., 2005. USDA National Nutrient Database for Standard Reference, Release No. 18. Home Page: www.ars.usda.gov/nutrientdata
Hoyle, N. T., and Merrltt, J. H., 1994. Quality of fish protein hydrolysates from herring (Clupea harengus). Journal of Food Science, 59(1), 76–79. DOI: https://doi.org/10.1111/j.1365-2621.1994.tb06901.x
Shahidi, F., Han, X. Q., and Synowiecki, J., 1995. Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food chemistry, 53(3), 285–293. DOI: https://doi.org/10.1016/0308-8146(95)93934-J
Benjakul, S., and Morrissey, M. T., 1997. Protein hydrolysates from Pacific whiting solid wastes. Journal of Agricultural and Food Chemistry, 45(9), 3423–3430. DOI: https://doi.org/10.1021/jf970294g
Kristinsson, H. G., and Rasco, B. A., 2000. Fish protein hydrolysates: production, biochemical, and functional properties. Critical Reviews in Food Science and Nutrition, 40(1), 43–81. DOI: https://doi.org/10.1080/10408690091189266
Guérard, F., Dufosse, L., de La Broise, D., and Binet, A., 2001. Enzymatic hydrolysis of proteins from yellowfin tuna (Thunnus albacares) wastes using Alcalase. Journal of molecular catalysis B: Enzymatic, 11(4–6), 1051–1059. DOI: https://doi.org/10.1016/S1381-1177(00)00031-X
Nielsen, P. M., Petersen, D., and Dambmann, C. J. J. O. F. S., 2001. Improved method for determining food protein degree of hydrolysis. Journal of Food Science, 66(5), 642–646. DOI: https://doi.org/10.1111/j.1365-2621.2001.tb04614.x
Bligh, E. G., and Dyer, W. J., 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911–917. DOI: https://doi.org/10.1139/o59-099
Bradford, M. M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254. DOI: https://doi.org/10.1006/abio.1976.9999
Ma, X., Zhao, D., Li, X., and Meng, L., 2015. Chromatographic method for determination of the free amino acid content of chamomile flowers. Pharmacognosy Magazine, 11(41), 176–179. DOI: https://doi.org/10.4103/0973-1296.149735
Šelih, V. S., Šala, M., and Drgan, V., 2014. Multi-element analysis of wines by ICP-MS and ICP-OES and their classification according to geographical origin in Slovenia. Food Chemistry, 153, 414–423. DOI: https://doi.org/10.1016/j.foodchem.2013.12.081
Chen, D. W., Zhang, M., and Shrestha, S., 2007. Compositional characteristics and nutritional quality of Chinese mitten crab (Eriocheir sinensis). Food Chemistry, 103(4), 1343–1349. DOI: https://doi.org/10.1016/j.foodchem.2006.10.047
Alparslan, Y., Metin, C., and Baygar, T., 2022. Sex-and season-based comparison of lipid and fatty acid profiles of blue crab meat. Journal of Food & Nutrition Research, 61(1).
Ayas, D., and Özogul, Y., 2011. The chemical composition of carapace meat of sexually mature blue crab (Callinectes sapidus, Rathbun 1896) in the Mersin Bay. Journal of FisheriesSciences.com, 5(3), 262–269. DOI: https://doi.org/10.3153/jfscom.2011030
Kechaou, E. S., Dumay, J., Donnay-Moreno, C., Jaouen, P., Gouygou, J. P., Bergé, J. P., and Amar, R. B., 2009. Enzymatic hydrolysis of cuttlefish (Sepia officinalis) and sardine (Sardina pilchardus) viscera using commercial proteases: Effects on lipid distribution and amino acid composition. Journal of bioscience and bioengineering, 107(2), 158–164. DOI: https://doi.org/10.1016/j.jbiosc.2008.10.018
Nguyen, H. T. M., Sylla, K. S. B., Randriamahatody, Z., Donnay-Moreno, C., Moreau, J., Tran, L. T., and Bergé, J. P., 2011. Enzymatic hydrolysis of yellowfin tuna (Thunnus albacares) by-products using Protamex protease. Food Technology and Biotechnology, 49(1), 48–55.
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