Research on the growth ability of Saccharomyces cerevisiae MN2 in the medium containing different concentrations of NaCl and NH\(_{4}^{+}\) for applying in aquaculture

Mai Anh Thi Dang, Thu Minh Nguyen, Huong Thi Thu Luong, Nguyen Sy Nguyen, Cuong Tu Ho
Author affiliations

Authors

  • Mai Anh Thi Dang Institute of Environmental Technology, VAST, Vietnam
  • Thu Minh Nguyen Institute of Environmental Technology, VAST, Vietnam
  • Huong Thi Thu Luong Institute of Environmental Technology, VAST, Vietnam
  • Nguyen Sy Nguyen Institute of Environmental Technology, VAST, Vietnam
  • Cuong Tu Ho Institute of Environmental Technology, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/17506

Keywords:

NaCl concentration, NH4 concentration, Saccharomyces cerevisiae, aquacultural treatment.

Abstract

Salinity is one of the factors affecting the growth and activity of microorganisms. The study of the salinity effect can indicate the range of microorganism applications. This study evaluated the effects of NaCl concentration on growth, amino acid and NH4+ absorption of yeast strain Saccharomyces cerevisia MN2. The results showed that, within NaCl concentration from 0 to 3%, the negative effects on the growth of MN2 strain were not observed. The cell density on all culture media reached 108 CFU/ml after 24 hours and maintained until 48 hours.The strain NM2 was able to absorb amino acids at a high rate, which was indicated by the decrease of the amino acid concentration in the culture media after 48 hours (from 20.8 to 37.5%) in comparison with the control. In addition, the strain NM2 was also able to decrease NH4+ concentration in culture media, reducing from 17 to 25.4% in comparison with the control after 48 hours. Strain MN2 is capable of using ammonium as a nitrogen source. When the ammonium concentration fluctuated in the range of 37,327 - 75,433 mg/l, it did not affect the growth of MN2 much. In conclusion, the strain of S. cerevisia MN2 is tolerance to the salinity up to 3%, and suitable for the aquaculture wastewater treatment process.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Logothetis, S., Walker, G., and Nerantzis, E. T., 2007. Effect of salt hyperosmotic stress on yeast cell viability. Zbornik Matice srpske za prirodne nauke, (113), 271–284.

Logothetis, S., Nerantzis, E. T., Gioulioti, A., Kanelis, T., Panagiotis, T., and Walker, G., 2010. Influence of sodium chloride on wine yeast fermentation performance. International Journal of Wine Research, 2, 35–42.

Rostami, F., Davoodi, R., Nafisi Bahabadi, M., Salehi, F., and Nooryazdan, H., 2019. Effects of ammonia on growth and molting of Litopenaeus vannamei postlarvae reared under two salinity levels. Journal of Applied Aquaculture, 31(4), 309–321.

Meade, J. W., 1985. Allowable ammonia for fish culture. The Progressive Fish‐Culturist, 47(3), 135–145.

Robinette, H. R., 1976. Effect of selected sublethal levels of ammonia on the growth of channel catfish (Ictalurus punctatus). The Progressive Fish-Culturist, 38(1), 26–29.

Takagi, H., Shichiri, M., Takemura, M., Mohri, M., and Nakamori, S., 2000. Saccharomyces cerevisiae Σ1278b has novel genes of the N-acetyltransferase gene superfamily required for L-proline analogue resistance. Journal of Bacteriology, 182(15), 4249–4256.

Magasanik, B., 2003. Ammonia assimilation by Saccharomyces cerevisiae. Eukaryotic cell, 2(5), 827–829.

Marini, A. M., Soussi-Boudekou, S., Vissers, S., and Andre, B., 1997. A family of ammonium transporters in Saccharomyces cerevisiae. Molecular and cellular biology, 17(8), 4282–4293.

El-Samragy, Y. A., and Zall, R. R., 1988. The influence of sodium chloride on the activity of yeast in the production of single cell protein in whey permeate. Journal of dairy science, 71(5), 1135–1140.

Courchesne, W. E., and Magasanik, B. O. R. I. S., 1983. Ammonia regulation of amino acid permeases in Saccharomyces cerevisiae. Molecular and Cellular Biology, 3(4), 672–683.

Grenson, M., Dubois, E., Piotrowska, M., Drillien, R., and Aigle, M., 1974. Ammonia assimilation in Saccharomyces cerevisiae as mediated by the two glutamate dehydrogenases: Evidence for the gdh A locus being a structural gene for the NADP-dependent glutamate dehydrogenase. Molecular and General Genetics MGG, 128, 73–85.

Van Quyen, N., Thao, N. Q., Anh, N. T., and Dat, N. T., 2018. The influence of some factors on the production of alcohol whisky by Saccharomyces cerevisiae MS42 from barley malt. Vietnam Journal of Biotechnology, 16(3), 525–532.

Nguyen, P. M., and Dong, T. A. D., 2013. Investigation of Saccharomyces cerevisiae in fermented mulberry juice. Middle East Journal of Scientific Research, 17(6), 814–820.

Hang, P. T., Hong, T. T. T., Tao, T. S., Quan, N. H., Na, T. T., Thao, L. D., Shimamura, Y., Kamisoyama, H., and Hai, D. T., 2020. Fermenting rice bran and maize with Saccharomyces cerevisiae and feeding the fermented product to chickens. Livestock Research for Rural Development, 32, 23.

Ha, N. T., Hong, T. T., Nhan, N. T. T., Van, D. T. C., and Yen, L. T. T., 2007. Preliminary study on removal of some heavy metals (Cu2+, Pb2+, Zn2+) in water by biosorption using Saccharomyces cerevisiae yeast. VNU Journal of Science: Natural Science and Technology, 23, 99–106. (in Vietnamese).

Directorate for Standards, Metrology and Quality, Ministry of Science and Technology, 2012. TCVN 87:2012. (in Vietnamese).

Directorate for Standards, Metrology and Quality, Ministry of Science and Technology, 1996. TCVN 6179-1:1996. (in Vietnamese).

Oda, Y., and Tonomura, K., 1993. Sodium chloride enhances the potential leavening ability of yeast in dough. Food microbiology, 10(3), 249–254.

Ljungdahl, P. O., and Daignan-Fornier, B., 2012. Regulation of amino acid, nucleotide, and phosphate metabolism in Saccharomyces cerevisiae. Genetics, 190(3), 885–929.

Santos, J., Sousa, M. J., and Leão, C., 2012. Ammonium is toxic for aging yeast cells, inducing death and shortening of the chronological lifespan. PloS one, 7(5), e37090.

Downloads

Published

08-06-2023

How to Cite

Dang, T. M. A., Nguyen, T. M., Luong, H. T. T., Nguyen, N. S., & Ho, C. T. (2023). Research on the growth ability of Saccharomyces cerevisiae MN2 in the medium containing different concentrations of NaCl and NH\(_{4}^{+}\) for applying in aquaculture. Vietnam Journal of Marine Science and Technology, 23(2), 161–168. https://doi.org/10.15625/1859-3097/17506

Issue

Section

Articles