Comparision protocols for extraction of microplastics in water samples

Ngoc Dinh Hai; Nghi Duong Thanh; Thanh Nga Cao Thi; Phuong Quynh Le Thi; Oanh Doan Thi; Kien Nguyen Trung; Thuy Duong Thi

doi:10.15625/1859-3097/17430

Author affiliations

Authors

Dinh Hai Ngoc Institute of Marine Environment and Resources, VAST, Vietnam; Graduate University of Science and Technology, VAST, Vietnam
Duong Thanh Nghi Institute of Marine Environment and Resources, VAST, Vietnam
Cao Thi Thanh Nga Graduate University of Science and Technology, VAST, Vietnam; Institute of Human Geography, VAST, Vietnam
Le Thi Phuong Quynh Institute of Natural Products Chemistry, VAST, Vietnam
Doan Thi Oanh Faculty of Environment, Hanoi University of Natural Resources and Environment, Hanoi, Vietnam
Nguyen Trung Kien Institute of Environmental Technology, VAST, Vietnam
Duong Thi Thuy Graduate University of Science and Technology, VAST, Vietnam; Institute of Environmental Technology, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/17430

Abstract

Microplastics (MPs) are increasingly recognized as emerging pollutants in various environmental components. However, protocols for sampling, analysis, and standardization of measurements in MPs research are still under development. The extraction method is a crucial factor that affects the accuracy and comparability of microplastic abundance data. In this study, we evaluated and compared the effectiveness of four different extraction protocols (D, MJ, MA, and S) for separating MPs from water samples of different types (brackish, marine, and river water). Known combinations of MP particles (polyethylene (PE), polyethylene terephthalate (PET), polystyrene (PS), and polyvinyl chloride (PVC)) with sizes ranging between 150 μm and 700 μm were spiked into water samples. Our results showed that the average recovery efficiency of microplastics using the four studied methods ranged from 53% to 86%. Notably, the recovery efficiency of light-density MPs was higher than that of heavy-density MPs. For purified water samples (PW) obtained from a filtration system, only H₂O₂ was effective in recovering MPs with an efficiency of 80 ± 6.61%. The S method for MP extraction, which combines SDS, Bioenzyme, H2O2 30%, and a saturated salt solution using NaCl, gave the highest average MP recovery of 78.13 ± 2.39% in PW and 69.72 ± 4.81% in surface water. This method has several advantages over the other three methods, such as low cost, environmental friendliness, and compatibility with various water samples, making it suitable for analyzing large amounts of MPs. Our study highlights the importance of carefully selecting the appropriate extraction protocol for accurate and reliable microplastic analysis in different water samples.

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