Effect of dietary astaxanthin on reproductive performance, egg quality and larvae of clowfish Amphiprion ocellaris (Cuvier, 1830)

Nguyen Thi Nguyet Hue, Ho Son Lam, Dao Thi Hong Ngoc, Dang Tran Tu Tram, Huynh Minh Sang, Dinh Truong An, Doan Van Than, Nguyen Truong Tan Tai, Do Hai Dang, Hua Thai An
Author affiliations

Authors

  • Nguyen Thi Nguyet Hue Institute of Oceanography, VAST, Vietnam
  • Ho Son Lam Institute of Oceanography, VAST, Vietnam
  • Dao Thi Hong Ngoc Institute of Oceanography, VAST, Vietnam
  • Dang Tran Tu Tram Institute of Oceanography, VAST, Vietnam
  • Huynh Minh Sang Institute of Oceanography, VAST, Vietnam
  • Dinh Truong An Institute of Oceanography, VAST, Vietnam
  • Doan Van Than Institute of Oceanography, VAST, Vietnam
  • Nguyen Truong Tan Tai Institute of Oceanography, VAST, Vietnam
  • Do Hai Dang Institute of Oceanography, VAST, Vietnam
  • Hua Thai An Institute of Oceanography, VAST, Vietnam

Keywords:

Astaxanthin, nemo clownfish, reproductive quality, nutrition.

Abstract

This study was designed to evaluate the effects of astaxanthin in broodfish diets on reproductive performance, egg quality and larvae quality parameters of clownfish (Amphirion ocellaris). Five treatments were tested with 5 levels of astaxanthin (Carophyll Pink 10% CWS) of 0, 50, 100, 150 and 200 mg/kg added to the feed. Each treatment was repeated in triplicate and the supplemental feeding trials were arranged for 13 months. The results showed that there were significant differences in hatching rate of egg, malformed rate and survival rate of larvae in 3 days post-hatch (p<0.05) among the feeding trials of astaxanthin supplements. The highest hatching rate of egg and survival rate and the lowest malformed rate of larvae were observed in the treatment that was supplemented with astaxanthin 150 mg/kg feed, respectively 92.14 %; 93.57 % and 0.55 %. However, the astaxanthin supplementary diets did not affect the re-maturation and spawning period, spawning frequency, fecundity, egg diameter and larval size of nemo fish among the treatments. The results also suggested that astaxanthin requirement for clownfish broodstock to improve reproductive performance was 150 mg/kg feed.

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References

[1] Ha Le Thi Loc, 2010. Research on hatchery technology and and growth out culture of Nemo fish (Amphiprion ocellaris Cuvier, 1830) for exportation. Summary report on scientific and technological results of national level KC. 06.07/06-10.2010: 207 p. (in Vietnamese).

[2] Rainuzzo, J. R., Reitan, K. I., and Olsen, Y., 1997. The significance of lipids at early stages of marine fish: a review. Aquaculture, 155(1–4), 103–115. https://doi.org/10.1016/S0044-8486(97)00 121-X.

[3] Bruce, M., Oyen, F., Bell, G., Asturiano, J. F., Farndale, B., Carrillo, M., ... and Bromage, N., 1999. Development of broodstock diets for the European sea bass (Dicentrarchus labrax) with special emphasis on the importance of n-3 and n-6 highly unsaturated fatty acid to reproductive performance. Aquaculture, 177(1–4), 85–97. https://doi.org/10.1016/S0044-8486(99)00071-X.

[4] Hamre, K. Fernández-Palacios, H., Izquierdo, M. Norberg, B. and Hamre, K., 2011. Effects of Broodstock Diet on Eggs and Larvae. In: Larval Fish Nutrition. GJ Holt (Ed) Wiley-Blackwell, UK. pp. 153–182.

[5] Izquierdo, M. S., Fernandez-Palacios, H., and Tacon, A. G. J., 2001. Effect of broodstock nutrition on reproductive performance of fish. Aquaculture, 197(1–4), 25–42. https://doi.org/10.1016/S0044-8486(01)00581-6.

[6] Watanabe, T., and Vassallo-Agius, R., 2003. Broodstock nutrition research on marine finfish in Japan. Aquaculture, 227(1–4), 35–61. https://doi.org/10.1016/ S0044-8486(03)00494-0.

[7] Doolan, B. J., Allan, G. L., Booth, M. A., and Jones, P. L., 2008. Effect of carotenoids and background colour on the skin pigmentation of Australian snapper Pagrus auratus (Bloch & Schneider, 1801). Aquaculture Research, 39(13), 1423–1433. https://doi.org/10.1111/j.1365-2109.2008.02012.x.

[8] Ramamoorthy, K., Bhuvaneswari, S., Sankar, G., and Sakkaravarthi, K., 2010. Proximate composition and carotenoid content of natural carotenoid sources and its colour enhancement on marine ornamental fish Amphiprion ocellaris (Cuveir, 1880). World Journal of Fish and Marine Sciences, 2(6), 545–550.

[9] Salze, G., Tocher, D. R., Roy, W. J., and Robertson, D. A., 2005. Egg quality determinants in cod (Gadus morhua L.): egg performance and lipids in eggs from farmed and wild broodstock. Aquaculture Research, 36(15), 1488–1499. https://doi.org/10.1111/j.1365-2109.2005.01367.x.

[10] Ahmadi, M. R., Bazyar, A. A., Safi, S., Ytrestøyl, T., and Bjerkeng, B., 2006. Effects of dietary astaxanthin supplementation on reproductive characteristics of rainbow trout (Oncorhynchus mykiss). Journal of Applied Ichthyology, 22(5), 388–394. https://doi.org/10.1111/j.1439-0426.2006. 00770.x.

[11] Díaz-Jiménez, L., Hernández-Vergara, M. P., Pérez-Rostro, C. I., and Ortega-Clemente, L. A., 2019. The effect of astaxanthin and β-carotene inclusion in diets for growth, reproduction and pigmentation of the peppermint shrimp Lysmata wurdemanni. Latin American Journal of Aquatic Research, 47(3), 559–567.

/[12] Craik, J. C. A., 1985. Egg quality and egg pigment content in salmonid fishes. Aquaculture, 47(1), 61–88. https://doi.org/10.1016/0044-8486(85)900 08-0.

[13] Mikulin, A. E., 2000. Functional role of pigments and pigmentation in fish ontogeny. VNIRO, Moscow, 432.

[14] Edge, R., McGarvey, D. J., and Truscott, T. G., 1997. The carotenoids as anti-oxidants–a review. Journal of Photochemistry and Photobiology B: Biology, 41(3), 189–200. https://doi.org/10.1016/S1011-1344(97)00 092-4.

[15] Britton, G., Liaaen-Jensen, S., and Pfander, H. (Eds.), 2008. Carotenoids, Vol. 4: Natural functions. Springer Science & Business Media.

[16] Yasir, I., and Qin, J. G., 2010. Effect of dietary carotenoids on skin color and pigments of false clownfish, Amphiprion ocellaris, Cuvier. Journal of the World Aquaculture Society, 41(3), 308–318. https://doi.org/10.1111/j.1749-7345.2010. 00373.x

[17] Lam, H. S., Vy, N. T., and Ngoc, P. T., 2016. Effect of dietary astaxanthin on growth, survival rate and pigmentation of commercial clownfish, Amphiprion ocellaris. Vietnam Journal of Marine Science and Technology, 16(3), 321–327. https://doi.org/10.15625/1859-3097/16/3/7322.

[18] Ha Le Thi Loc, Nguyen Kim Bich, Nguyen Thi Thanh Thuy, Nguyen Trung Kien, 2012. Protocol of seed production and growth out culture of nemo fish (Amphiprion ocellaris Cuvier, 1830) for exportation. Proceedings of the International Conference on “Bien Dong 2012”, Nha Trang, 12–14 September 2012. pp. 262–268.

[19] Hoff, F. H., 1996. Conditioning, spawning and rearing of fish with emphasis on marine clownfish. Aquaculture Consultants, Incorporated.

[20] Madhu, R., Madhu, K., and Retheesh, T., 2012. Life history pathways in false clown Amphiprion ocellaris Cuvier, 1830: A journey from egg to adult under captive condition. Journal of the Marine Biological Association of India, 54(1), 77–90.

[21] Chou, Y. H., and Chien, Y. H., 2006. Effects of astaxanthin and vitamin E supplement in Japanese sea bass (Lateolabrax japonicus) broodstock diet on their spawning performance and egg quality. Journal of the Fisheries Society of Taiwan, 33(2), 157–169. Doi: 10.29822/JFST.200606.0007.

[22] Tizkar, B., Soudagar, M., Bahmani, M., Hosseini, S. A., and Chamani, M., 2013. The Effects of Dietary Supplementation of Astaxanthin and Β-caroten on the Reproductive Performance and Egg Quality of Female Goldfish (Carassius auratus). Caspian J. Env. Sci., 11(2), 217–231.

[23] Hansen, Ø. J., Puvanendran, V., and Bangera, R., 2016. Broodstock diet with water and astaxanthin improve condition and egg output of brood fish and larval survival in Atlantic cod, Gadus morhua L. Aquaculture Research, 47(3), 819–829. https://doi.org/10.1111/are.12540.

[24] Ignatius, B., Rathore, G., Jagadis, I., Kandasamy, D., & Victor, A. C. C. (2001). Spawning and larval rearing technique for tropical clown fish Amphiprion sebae under captive condition. Journal of Agriculture in Tropics, 16(3), 241-249.

[25] Madhu, K., Madhu, R., Gopakumar, G., and Sasidharan, C. S., 2006. Breeding, larval rearing and seed production of maroon clown Premnas biaculeatus under captive conditions. Marine Fisheries Information Service, Technical and Extension Series, 190, 1–5.

[26] Anil, M. K., Santhosh B., Jasmine. S, Reenamole, S., Unnikrishnan, C. and Anukumar, A., 2010. Techniques for mass production of two species of clown fish: Clown anemonefish Amphiprion ocellaris Cuvier, 1830 and Spinecheek anemonefish Premnas biaculeatus (Bloch, 1790). In: Felix, S. (Ed.), The proceedings of the National Seminar on technology and trade prospects in Ornamental aquaculture, Fisheries Research and Extension Centre, Tamil Nadu Veterinary and Animal Sciences University, Chennai, pp. 96–102.

[27] Smith, C., and Reay, P., 1991. Cannibalism in teleost fish. Reviews in Fish Biology and Fisheries, 1(1), 41–64. https://doi.org/10.1007/BF00042661.

[28] Hoelzer, G. (1992). The ecology and evolution of partial-clutch cannibalism by paternal Cortez damselfish. Oikos, 65(1), 113–120. Doi: 10.2307/3544893.

[29] Kraak, S. B., 1996. Female preference and filial cannibalism in Aidablennius sphynx (Teleostei, Blenniidae); a combined field and laboratory study. Behavioural Processes, 36(1), 85–97. https://doi.org/10.1016/0376-6357(95)00019-4.

[30] Kvarnemo, C., Svensson, O. L. A., and Forsgren, E., 1998. Parental behaviour in relation to food availability in the common goby. Animal Behaviour, 56(5), 1285–1290. https://doi.org/10.1006/anbe.1998.0899.

[31] Lindström, K., and Sargent, R. C., 1997. Food access, brood size and filial cannibalism in the fantail darter, Etheostoma flabellare. Behavioral Ecology and Sociobiology, 40(2), 107–110. https://doi.org/10.1007/s00265005 0322.

[32] Belles-Isles, J. C., and FitzGerald, G. J., 1991. Filial cannibalism in sticklebacks: a reproductive management strategy?. Ethology Ecology & Evolution, 3(1), 49–62. https://doi.org/10.1080/08927014.1991.9525388.

[33] Pangantihon-Kühlmann, M. P., Millamena, O., and Chern, Y., 1998. Effect of dietary astaxanthin and vitamin A on the reproductive performance of Penaeus monodon broodstock. Aquatic Living Resources, 11(6), 403–409. https://doi.org/10.1016/S0990-7440(99)80006-0.

[34] Steven, D. M., 1949. Studies on animal carotenoids: II. Carotenoids in the reproductive cycle of the brown trout. Journal of experimental Biology, 26(3), 295–303.

[35] Bjerkeng, B., Storebakken, T., and Liaaen-Jensen, S., 1992. Pigmentation of rainbow trout from start feeding to sexual maturation. Aquaculture, 108(3-4), 333–346. https://doi.org/10.1016/0044-8486(92)90117-4.

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Published

11-04-2021

How to Cite

Hue, N. T. N., Lam, H. S., Ngoc, D. T. H., Tram, D. T. T., Sang, H. M., An, D. T., Than, D. V., Tai, N. T. T., Dang, D. H., & An, H. T. (2021). Effect of dietary astaxanthin on reproductive performance, egg quality and larvae of clowfish <i>Amphiprion ocellaris</i> (Cuvier, 1830). Vietnam Journal of Marine Science and Technology, 20(4A), 163–172. Retrieved from https://vjs.ac.vn/index.php/jmst/article/view/15644

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Vol. 20 No. 4A (2020)

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