BIODIVERSITY ASSESSMENT OF MICROORGANISMS ASSOCIATED WITH TWO MARINE SPONGES (Haliclona oculata AND Amphius huxleyi) COLLECTED AT THE LANG CO BAY OF VIETNAM

Ton That Huu Dat; Pham Viet Cuong; Tran Thi Dung Dung; Vu Thi Thu Huyen; Nguyen Thi Kim Cuc

doi:10.15625/1859-3097/18/3/10642

Author affiliations

Authors

Ton That Huu Dat Mientrung Institute for Scientific Research, VAST, Vietnam
Pham Viet Cuong Mientrung Institute for Scientific Research, VAST, Vietnam
Tran Thi Dung Dung Mientrung Institute for Scientific Research, VAST, Vietnam
Vu Thi Thu Huyen Institute of Marine Biochemistry, VAST, Vietnam
Nguyen Thi Kim Cuc Institute of Marine Biochemistry, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/18/3/10642

Keywords:

Amphius huxleyi, Haliclona oculata, Illumina Miseq system, sponge-associated microorganisms.

Abstract

Sponges (Phylum Porifera) are ancient sedentary and filter-feeding animals which harbour very diverse and abundant associated microbial community in their tissues with density up to 40–50% of sponge tissue volume. In this study, the diversity of associated microorganisms with two marine sponges Haliclona oculata and Amphius huxleyi collected at the Lang Co bay of Vietnam was assessed by analysis of hypervariable V3 and V4 regions of the 16S rRNA gene using Illumina MiSeq system. The taxonomic diversity of sponge-associated microorganisms was classified to different taxonomic levels (kingdom, phylum, class, order, family, and genus). Based on Bayesian classification method and reference sequences derived from Greengenes database, the associated microorganisms in studied sponges were assigned to 17 phyla (H. oculata) and 13 phyla (A. huxleyi). Many microbial taxa were detected in two sponge species, however, they were distinctive by the abundance. Proteobacteria was the most dominant phylum in both sponge species, and all of 4 classes Epsilonproteobacteria, Gammaproteobacteria, Alphaproteobacteria, and Deltaproteobacteria were found in H. oculata and A. huxleyi.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Hentschel, U., Usher, K. M., and Taylor, M. W., 2006. Marine sponges as microbial fermenters. FEMS Microbiology Ecology, 55(2), 167–177. doi.org/10.1111/j.1574-6941.2005.00046.x.

Lee, Y. K., Lee, J. H., and Lee, H. K., 2001. Microbial symbiosis in marine sponges. The Journal of Microbiology, 39(4), 254–264.

Weisz, J. B., Hentschel, U., Lindquist, N., and Martens, C. S., 2007. Linking abundance and diversity of sponge-associated microbial communities to metabolic differences in host sponges. Marine Biology, 152(2), 475–483.

White, J. R., Patel, J., Ottesen, A., Arce, G., Blackwelder, P., and Lopez, J. V., 2012. Pyrosequencing of bacterial symbionts within Axinella corrugata sponges: diversity and seasonal variability. PloS One, 7(6), e38204. doi.org/10.1371/journal.pone.0038204.

Erwin, P. M., Olson, J. B., and Thacker, R. W., 2011. Phylogenetic diversity, host-specificity and community profiling of sponge-associated bacteria in the northern Gulf of Mexico. PloS One, 6(11), e26806. doi.org/10.1371/journal.pone.0026806.

Hardoim, C. C., Esteves, A. I., Pires, F. R., Gonçalves, J. M., Cox, C. J., Xavier, J. R., and Costa, R., 2012. Phylogenetically and spatially close marine sponges harbour divergent bacterial communities. PLoS One, 7(12), e53029. doi.org/ 10.1371/journal.pone.0053029.

Olsen, G. J., Lane, D. J., Giovannoni, S. J., Pace, N. R., & Stahl, D. A. (1986). Microbial ecology and evolution: a ribosomal RNA approach. Annual reviews in microbiology, 40(1), 337–365. doi.org/10.1146/annurev.mi.40.100186.002005.

Andersson, A. F., Lindberg, M., Jakobsson, H., Bäckhed, F., Nyrén, P., and Engstrand, L., 2008. Comparative analysis of human gut microbiota by barcoded pyrosequencing. PloS One, 3(7), e2836. doi.org/10.1371/journal.pone.0002836.

Herlemann, D. P., Labrenz, M., Jürgens, K., Bertilsson, S., Waniek, J. J., and Andersson, A. F., 2011. Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea. The ISME journal, 5(10), 1571–1579. doi.org/10.1038/ismej.2011.41.

Ouyang, Y., Dai, S., Xie, L., Kumar, M. R., Sun, W., Sun, H., Tang, D., and Li, X., 2010. Isolation of high molecular weight DNA from marine sponge bacteria for BAC library construction. Marine Biotechnology, 12(3), 318–325. doi.org/ 10.1007/s10126-009-9223-0.

Hill, M., Hill, A., Lopez, N., and Harriott, O., 2006. Sponge-specific bacterial symbionts in the Caribbean sponge, Chondrilla nucula (Demospongiae, Chondrosida). Marine Biology, 148(6), 1221–1230. doi.org/10.1007/s00227-005-0164-5.

Simister, R. L., Deines, P., Botté, E. S., Webster, N. S., and Taylor, M. W., 2012. Sponge‐specific clusters revisited: a comprehensive phylogeny of sponge‐associated microorganisms. Environmental Microbiology, 14(2), 517–524. doi.org/10.1111/j.1462-2920.2011.02664.x.

Pita, L., Turon, X., López-Legentil, S., and Erwin, P. M., 2013. Host rules: spatial stability of bacterial communities associated with marine sponges (Ircinia spp.) in the Western Mediterranean Sea. FEMS Microbiology Ecology, 86(2), 268–276. doi.org/10.1111/1574-6941.12159.

Degnan, P. H., and Ochman, H., 2012. Illumina-based analysis of microbial community diversity. The ISME journal, 6(1), 183–194. doi.org/10.1038/ismej.20 11.74.

Caporaso, J. G., Lauber, C. L., Walters, W. A., Berg-Lyons, D., Lozupone, C. A., Turnbaugh, P. J., Fierer, N., and Knight, R., 2011. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proceedings of the National Academy of Sciences, 108(Supplement 1), 4516–4522. doi.org/10.1073/pnas.1000080107.

Gloor, G. B., Hummelen, R., Macklaim, J. M., Dickson, R. J., Fernandes, A. D., MacPhee, R., and Reid, G., 2010. Microbiome profiling by illumina sequencing of combinatorial sequence-tagged PCR products. PloS One, 5(10), e15406. doi.org/10.1371/journal.pone.00 15406.

Öztürk, B., De Jaeger, L., Smidt, H., and Sipkema, D., 2013. Culture-dependent and independent approaches for identifying novel halogenases encoded by Crambe crambe (marine sponge) microbiota. Scientific reports, 3, 2780. doi.org/10.1038/srep02780.

Reveillaud, J., Maignien, L., Eren, A. M., Huber, J. A., Apprill, A., Sogin, M. L., and Vanreusel, A., 2014. Host-specificity among abundant and rare taxa in the sponge microbiome. The ISME journal, 8(6), 1198–1209. doi.org/10.1038/ismej.2013.227.

Webster, N. S., and Taylor, M. W., 2012. Marine sponges and their microbial symbionts: love and other relationships. Environmental microbiology, 14(2), 335–346. doi.org/10.1111/j.1462-2920.2011.02460.x.

Alex, A., and Antunes, A., 2015. Pyrosequencing characterization of the microbiota from Atlantic intertidal marine sponges reveals high microbial diversity and the lack of co-occurrence patterns. PLoS One, 10(5), e0127455. doi.org/10.1371/journal.pone.0127455.

Jasmin, C., Anas, A., and Nair, S., 2015. Bacterial diversity associated with Cinachyra cavernosa and Haliclona pigmentifera, cohabiting sponges in the coral reef ecosystem of Gulf of Mannar, Southeast Coast of India. PloS One, 10(5), e0123222. doi.org/10.1371/journal.pone. 0123222.

Sipkema, D., Holmes, B., Nichols, S. A., & Blanch, H. W. (2009). Biological characterisation of Haliclona (? gellius) sp.: sponge and associated microorganisms. Microbial Ecology, 58(4), 903–920. doi.org/10.1007/s00248-009-9534-8.

Abe, T., Sahin, F. P., Akiyama, K., Naito, T., Kishigami, M., Miyamoto, K., Sakakibara, Y., and Uemura, D., 2012. Construction of a metagenomic library for the marine sponge Halichondria okadai. Bioscience, Biotechnology, and Biochemistry, 76(4), 633–639. doi.org/10.1271/bbb.110533.

Chasanah, E., Patantis, G., Dewi, A. S., Marraskuranto, E., Januar, H. I., Stella, S., Soka, S., and Yogiara, Y., 2013. Analysis of Bacterial Community Associated with Aaptos sp. from Rote and Seribu Islands. Microbiology Indonesia, 7(1), 37–44. dx.doi.org/10.5454/mi.7.1.5.

Freeman, C. J., and Thacker, R. W., 2011. Complex interactions between marine sponges and their symbiotic microbial communities. Limnology and Oceanography, 56(5), 1577–1586. doi.org/10.4319/lo.2011.56.5.1577.

Giles, E. C., Kamke, J., Moitinho-Silva, L., Taylor, M. W., Hentschel, U., Ravasi, T., and Schmitt, S., 2013. Bacterial community profiles in low microbial abundance sponges. FEMS Microbiology Ecology, 83(1), 232–241. doi.org/10.1111/j.1574-6941.2012.01467.x.

Lee, O. O., Wang, Y., Yang, J., Lafi, F. F., Al-Suwailem, A., and Qian, P. Y., 2011. Pyrosequencing reveals highly diverse and species-specific microbial communities in sponges from the Red Sea. The ISME journal, 5(4), 650–644. doi.org/10.1038/ismej.2010.165.

Naim, M. A., Morillo, J. A., Sørensen, S. J., Waleed, A. A. S., Smidt, H., and Sipkema, D., 2014. Host-specific microbial communities in three sympatric North Sea sponges. FEMS Microbiology Ecology, 90(2), 390–403. doi.org/10.1111/1574-6941.12400.

Schläppy, M. L., Schöttner, S. I., Lavik, G., Kuypers, M. M., de Beer, D., and Hoffmann, F., 2010. Evidence of nitrification and denitrification in high and low microbial abundance sponges. Marine biology, 157(3), 593–602.