Dinesh. S, Roohi Fatima. M, Komal B Patil, Kanika Verma, Noopur Gupta, Liz Thenamkodath, Priyanka Menon, Mekata. T, Itami. T, Sudhakaran. R


White spot syndrome virus (WSSV) is the most lethal pathogenic virus affecting the penaeid shrimp. Outbreak of WSSV causes high mortality among the populations of cultured penaeid shrimp. Aim of the present study was to diagnose the WSSV in early stage of infection. Immediate early genes are genes that are activated due to presence of cellular stimuli and have significant role in replication and proliferation of virus. In this study, wsv303 and wsv477 genes were chosen for analysis. This study demonstrates the detection of WSSV using primers designed from early genes and sequences the Indian isolates for homology analysis. Polymerase chain reaction was performed for the detection confirmation and the PCR products were cloned. Different organs such as gill, head soft tissue, heart tissue, intestine and tail tissue had been used for the PCR assay. Both genes were amplified at the size of 420 bp and 457 bp, respectively. Different duration samples of WSSV post-infection muscle DNAs were analyzed with the two primers and compared with OIE-nested PCR method convincing the early detecting ability of the virus. Sequencing analysis was performed with other isolates from France, China, The Netherlands and Taiwan. Phylogenetic analysis revealed that the Indian isolate was closely related to other isolates.


White spot syndrome virus, high mortality, early gene, wsv303 and wsv477 genes, Penaeid shrimp, single step PCR, the Indian isolate.

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Wu, W., Wang, L., and Zhang, X., 2005. Identification of white spot syndrome virus (WSSV) envelope proteins involved in shrimp infection. Virology, 332(2): 578-583.

Leu, J. H., Yang, F., Zhang, X., Xu, X., Kou, G. H., and Lo, C. F., 2009. Whispovirus. In Lesser Known Large dsDNA Viruses (pp. 197-227). Springer Berlin Heidelberg.

Natividad, K. D. T., Nomura, N., and Matsumura, M., 2008. Detection of White spot syndrome virus DNA in pond soil using a 2-step nested PCR. Journal of virological methods, 149(1): 28-34.

Wang, Y. G., Hassan, M. D., Shariff, M., Zamri, S. M., and Chen, X., 1999. Histopathology and cytopathology of white spot syndrome virus (WSSV) in cultured Penaeus monodon from peninsular Malaysia with emphasis on pathogenesis and the mechanism of white spot formation. Diseases of aquatic organisms, 39(1): 1-11.

Sudheer, N. S., Philip, R., and Singh, I. B., 2012. Anti-white spot syndrome virus activity of Ceriops tagal aqueous extract in giant tiger shrimp Penaeus monodon. Archives of virology, 157(9): 1665-1675.

Jensen, O. N., Houthaeve, T., Shevchenko, A., Cudmore, S., Ashford, T. O. N. Y., Mann, M. A. T. T. H. I. A. S., Griffiths, G., and Locker, J. K., 1996. Identification of the major membrane and core proteins of vaccinia virus by two-dimensional electrophoresis. Journal of virology, 70(11): 7485-7497.

Ma, G., Yu, L., Wang, Q., Liu, W., Cui, Y., and Kwang, J., 2012. Sf-PHB2, A new transcription factor, Drives WSSV Ie1 Gene Expression via a 12-bp DNA Element. Virology journal, 9(1): 206.

Liu, W. J., Chang, Y. S., Wang, C. H., Kou, G. H., and Lo, C. F., 2005. Microarray and RT-PCR screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp. Virology, 334(2): 327-341.

Li, L., Lin, S., and Yanga, F., 2005. Functional identification of the non-specific nuclease from white spot syndrome virus. Virology, 337(2): 399-406.

Chang, P. S., Chen, L. J., and Wang, Y. C., 1998. The effect of ultraviolet irradiation, heat, pH, ozone, salinity and chemical disinfectants on the infectivity of white spot syndrome baculovirus. Aquaculture, 166(1): 1-17.

Okano, K., Mikhailov, V. S., and Maeda, S., 1999. Colocalization of baculovirus IE-1 and two DNA-binding proteins, DBP and LEF-3, to viral replication factories. Journal of virology, 73(1): 110-119.

Lo, C. F., Ho. C., Peng, S., Chen, C. H., Hsu, H., Chiu, Y., Chang, C., Liu, K., Su, M., Wang, C., 1996. White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Diseases of Aquatic Organisms, 27, 215-225.

Lo, C. F., Leu, J. H., Ho, C. H., Chen, C. N., Peng, S. E., Chen, Y. T., Chou,C. M., Yeh, P. Y., Huang, C. J., Chou, H. Y., Wang, C. H., and Kou, G. H., 1996. Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. Diseases of aquatic organisms, 25, 133-141.

Sánchez-Paz, A., 2010. White spot syndrome virus: an overview on an emergent concern. Veterinary research, 41(6): 43.

Manjanaik, B., Umesha, K. R., Karunasagar, I., and Karunasagar, I., 2005. Detection of hepatopancreatic parvovirus (HPV) in wild shrimp from India by nested polymerase chain reaction (PCR). Diseases of aquatic organisms, 63, 255-259.

Han, F., Xu, J., and Zhang, X., 2007. Characterization of an early gene (wsv477) from shrimp white spot syndrome virus (WSSV). Virus genes, 34(2): 193-198.

Lin, F., Huang, H., Xu, L., Li, F., and Yang, F., 2011. Identification of three immediate-early genes of white spot syndrome virus. Archives of virology, 156(9): 1611-1614.

Tamura, K., Dudley, J., Nei, M., and Kumar, S., 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular biology and evolution, 24(8): 1596-1599.

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