The effects of <i>Gymnema sylvestre</i> extract supplementation on expression of gene associated with glucose homeostasis in diabetic mouse model.

Duc Quan Nguyen; Thi Kim Anh  Tran; Thi Trang Do; Thi Huong Giang Tran; Thi Thanh Trung Do; Tat Thanh Le; Huy Hoang Nguyen

doi:10.15625/vjbt-23222

Author affiliations

Authors

Duc Quan Nguyen \(^1\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam https://orcid.org/0000-0002-8152-5700
Thi Kim Anh Tran \(^2\) School of Life and Environmental Sciences & Sydney Institute of Agriculture, The University of Sydney, 1 Central Avenue, Eveleigh, NSW, Australia, 2015 https://orcid.org/0000-0002-6689-9077
Thi Trang Do \(^1\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
\(^3\) Publishing House for Science Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Thi Huong Giang Tran \(^1\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Thi Thanh Trung Do Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Tat Thanh Le \(^1\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Huy Hoang Nguyen \(^1\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
\(^4\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam

DOI:

https://doi.org/10.15625/vjbt-23222

Keywords:

Diabetes mellitus, Gymnema sylvestre, gymnemic acid, Mus musculus, RT-qPCR analysis.

Abstract

Gymnema sylvestre, a traditional medicinal herb known for its anti-diabetic properties, has been widely used to manage hyperglycemia by various mechanisms, including the inhibition of glucose absorption, stimulation of insulin secretion, and enhancement of pancreatic beta-cell regeneration. In the context of the increasing prevalence of diabetes, particularly in Vietnam, this study investigates the molecular effects of G. sylvestre extract (gymnemic acid) on gene expression associated with glucose homeostasis in diabetic mouse models induced by streptozotocin. The expression levels of key regulators involved in glucose transport, insulin production, and inflammatory responses, such as GLUT2, MAFA, INS2, PDX1, NFKB, and GCK, were evaluated using RT-qPCR analysis. Our findings indicated that diabetic mice, receiving 250 mg of G. sylvestre extract per kg of body weight daily for 6 weeks, exhibited significant improvements in glucose regulation when compared to their counterparts on a high-fat diet. This result was evidenced by increased gene expression related to insulin secretion and glucose uptake, alongside a decrease in body weight and blood glucose levels in diabetic mice. These beneficial outcomes can be attributed to the role of gymnemic acid in supporting various processes, including competitive inhibition of glucose absorption, enhancement of insulin secretion, protection and regeneration of pancreatic beta-cells, and improvement of lipid metabolism. Collectively, these findings highlight the potential of G. sylvestre as a nutrigenomic intervention in diabetes management through its regulation of key metabolic pathways.

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