Serogroups and <i>Neisseria meningitidis PORA, FETA</i> and <i>FHBP</i> gene distribution in Vietnam from 2014 to 2024

Phi Long Trieu; Viet Cuong Vo; Thi Lan Anh Bui; Van Toan Trinh; Thi Thanh Nga Bui; Dang Hieu Hoang; Van Tong Hoang; Van Quyen Dong; Huy Hoang Nguyen

doi:10.15625/vjbt-22546

Author affiliations

Authors

Phi Long Trieu \(^1\) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
\(^5\) Military Institute of Preventive Medicine, 21 Trung Liet, Dong Da, Hanoi, Vietnam
Viet Cuong Vo \(^2\) Institute of Tropical Medicine, Joint Vietnam-Russia Tropical Science and Technology Research Center, Viet Nam, 63 Nguyen Van Huyen, Hanoi, Vietnam https://orcid.org/0000-0001-5147-7085
Thi Lan Anh Bui \(^2\) Institute of Tropical Medicine, Joint Vietnam-Russia Tropical Science and Technology Research Center, Viet Nam, 63 Nguyen Van Huyen, Hanoi, Vietnam
Van Toan Trinh \(^2\) Institute of Tropical Medicine, Joint Vietnam-Russia Tropical Science and Technology Research Center, Viet Nam, 63 Nguyen Van Huyen, Hanoi, Vietnam
Thi Thanh Nga Bui \(^2\) Institute of Tropical Medicine, Joint Vietnam-Russia Tropical Science and Technology Research Center, Viet Nam, 63 Nguyen Van Huyen, Hanoi, Vietnam
Dang Hieu Hoang \(^2\) Institute of Tropical Medicine, Joint Vietnam-Russia Tropical Science and Technology Research Center, Viet Nam, 63 Nguyen Van Huyen, Hanoi, Vietnam
Van Tong Hoang \(^3\) Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, 222 Phung Hung, Ha Dong, Hanoi, Vietnam
Van Quyen Dong \(^4\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Huy Hoang Nguyen \(^4\) Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam

DOI:

https://doi.org/10.15625/vjbt-22546

Keywords:

Factor H binding protein, genosubtype, invasive meningococcal disease, outer membrane protein porin A, serogroup.

Abstract

Invasive meningococcal disease (IMD), caused by Neisseria meningitidis, is a continued public health concern. Invasive meningococcal disease, caused by Neisseria meningitidis, is a continued public health concern. Of the 12 N. meningitidis serogroups, the majority of invasive disease is caused by serogroups A, B, C, Y and W. This study aimed to investigate the serogroups, subgenotype, and fHbp antigenic profiles of N. meningitidis strains isolated in Vietnam from 2014 to 2023. Serogrouping of N. meningitidis by multiplex PCR assay and the genetic profiles including the porA, fHbp genes were analyzed using Sanger sequencing and bioinformatic methods. Of the 186 strain isolates, the most common serogroups were B (161 isolates, 86.6%), following serogroup B (5, 2.7%) and the remaining rates belongs to the other serogroups except serogroups A, B, C, W135, and Y. A total of 32 different combinations of VR1/VR2 PorA were identified in this study including 13 VR1 families and 21 VR2. The most common VR1 families were P1.22-25 (n = 36; 23.2%) followed by P1.7-2 (n= 31; 20.0%), P1.22 (n = 24; 15.5%), P1.22-11 (n = 19; 12.3%). As for VR2, the most frequent variants were 14 (n = 60, %), 15-25 (n = 20; 21.67%). The three most common profiles of porA gene were P1.22-25,14,36 (n = 24), P1.22,14,36 (n = 22), and P1.22-11,15-25,36 (n = 19). FHbp variant group 2 was most common (n = 142; 97.3%), followed by group 1 (n = 4; 2.7%), meaning a similar number of subfamily A (n = 142, being variant group 2) and B (n = 4, being variant group 1). 5 variants of subfamily A were found. The A22 variant was the most frequent (n = 52; 19.7%). On the basis of genotypic data showed poor matching the PorA and subfamily B FHbp component of the multicomponent 4CMenB Novartis meningococcal B vaccine.

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