Evaluation of the protection of astaxanthin on human hair follicle palilla cells against the aging effects triggered by hydrogen peroxide

Nguyen Thi Mong Thu; Nguyen Phan Nhu Quynh; Dang Nguyen Tuong Van; Luu Tran Thanh Uyen; Ho Thien Hoang; Le Thanh Long; Ho Nguyen Quynh Chi; To Minh Quan

doi:10.15625/vjbt-20532

Author affiliations

Authors

Nguyen Thi Mong Thu \(^1\) University of Science – VNUHCM;227 Nguyen Van Cu Std., Ward 4, District 5, Ho Chi Minh City, Vietnam.
Nguyen Phan Nhu Quynh \(^1\) University of Science – VNUHCM;227 Nguyen Van Cu Std., Ward 4, District 5, Ho Chi Minh City, Vietnam.
Dang Nguyen Tuong Van \(^3\) School of Medicine – Vietnam National University Ho Chi Minh City, YA1 Administrative Building, Hai Thuong Lan Ong Street, Dong Hoa Ward, Di An City, Binh Duong Province, Vietnam.
Luu Tran Thanh Uyen \(^1\) University of Science – VNUHCM;227 Nguyen Van Cu Std., Ward 4, District 5, Ho Chi Minh City, Vietnam.
Ho Thien Hoang \(^4\) Industrial University of Ho Chi Minh city, 12 Nguyen Van Bao Street, Ward 4, Go Vap District, Vietnam. https://orcid.org/0000-0002-3706-7917
Le Thanh Long \(^2\) Institute of Tropical Biology - Vietnam Academy of Science and Technology, 9/621 Hanoi highway, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.
Ho Nguyen Quynh Chi \(^2\) Institute of Tropical Biology - Vietnam Academy of Science and Technology, 9/621 Hanoi highway, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.
To Minh Quan \(^1\) University of Science – VNUHCM;227 Nguyen Van Cu Std., Ward 4, District 5, Ho Chi Minh City, Vietnam.

DOI:

https://doi.org/10.15625/vjbt-20532

Keywords:

Anti-aging, Astaxanthin (ASX), Dermal papillae, Oxidative Stress.

Abstract

Astaxanthin (ASX) is a powerful antioxidant with high efficacy in inhibiting reactive oxygen species (ROS), one of the most harmful factors leading to hair loss. Our research specifically evaluated the ability of ASX to protect human dermal papillae cells from hydrogen peroxide (H₂O₂). In this study, the human dermal papillae (hDP) cells were successfully isolated. The cells expressed DP cell markers in the 2D model, including alkaline phosphatase, alpha-smooth muscle actin (98.66 ± 0.02%), and vimentin (98.88 ± 0.02%), cluster aggregation and spheroid formation in the 3D model. The hDP cells (P3-P5) were pretreated with ASX and subsequently treated with H₂O₂ at a concentration of 550 µM for 4 hours. After 4 days of treatment, the percentage of cells expressing the marker b-galactosidase decreased from 87 ± 0.01% in the control group to 27.61 ± 0.01% in the ASX-treated group (1 µg/mL). Our results indicated that astaxanthin effectively protects against oxidative stress in dermal papillae cells, suggesting its potential application in hair loss treatment due to ROS.

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