Sequencing and analysis of the Meq oncogene of MDV causing marek’s disease in Bac Ninh province from 2019 to 2022

Doan Thi Thanh Huong, Nguyen Thi Thu Hien, Do Thi Roan, Luu Minh Duc, Le Thi Hue, Le Thanh Hoa, Le Thi Kim Xuyen, Nguyen Thi Khue
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Authors

  • Doan Thi Thanh Huong Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Thi Thu Hien Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam https://orcid.org/0000-0003-4940-4746
  • Do Thi Roan Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam https://orcid.org/0000-0002-2522-9229
  • Luu Minh Duc Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Le Thi Hue Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Le Thanh Hoa Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Le Thi Kim Xuyen Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Thi Khue Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam https://orcid.org/0000-0002-0158-973X

DOI:

https://doi.org/10.15625/1811-4989/18177

Abstract

Marek’s disease is a contagious avian viral disease that is caused by a Marek’s disease virus serotype 1 (MDV-1) or gallid herpesvirus 2 (GaHV-2), a member of the genus Alphaherpesvirus, family Herpesviridae. Up to now, the disease has caused significant losses to the chicken farming industry in many provinces and cities across the country. In this study, four MDV-1 (GaHV-2) strains causing the disease in Bac Ninh province during the 2019–2022 period were identified by multiplex Polymerase Chain Reaction (PCR). Subsequently, the segments of 1020 bp encoding 339 amino acids of Meq gene (Marek’s disease virus EcoRI fragment Q) from four samples were collected and sequenced. The rate of identity and homology between the four Vietnamese strains and other global strains ranged from 89.4% to 100% and 87.6% to 100%, respectively. These rates were higher between the four Vietnamese strains and MDV-1 Chinese strains, ranging from 99.2% to 100% and 98.2% to 100%, respectively. The molecular characterization of the Meq gene revealed that all four of the GaHV-2 strains infecting chickens in the Bac Ninh province belonged to the highly virulent group, with a low proline ratio in Meq protein (ranging from 20.59–21.18%), consisting of three PPPP motifs and three interrupted motifs that contain mutations at the second position of the proline rich region (PRR): PPPP>P (Q/A/R)PP. Overall, this study provides valuable information on molecular characteristics of MDV-1 strains in Bac Ninh province during the 2019–2022 period.

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Published

30-06-2023

How to Cite

Thanh Huong, D. T., Thu Hien, N. T., Roan, D. T., Minh Duc, L., Hue, L. T., Thanh Hoa, L., Kim Xuyen, L. T., & Khue, N. T. (2023). Sequencing and analysis of the Meq oncogene of MDV causing marek’s disease in Bac Ninh province from 2019 to 2022. Vietnam Journal of Biotechnology, 21(2), 281–291. https://doi.org/10.15625/1811-4989/18177

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