Indigenous diazotrophs and their effective properties for organic agriculture

Nguyen Thi Hieu Thu, Trinh Cao Son, Dang Thu Trang, Nguyen Thi My Le, Nguyen Duy Toi, Nguyen Thi Van, Dinh Thuy Hang
Author affiliations

Authors

  • Nguyen Thi Hieu Thu Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, E2 Building, 144 Xuan Thuy Road, Cau Giay District, Hanoi, Vietnam
  • Trinh Cao Son Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, E2 Building, 144 Xuan Thuy Road, Cau Giay District, Hanoi, Vietnam
  • Dang Thu Trang University of Science, Vietnam National University Hanoi, 334 Nguyen Trai Road, Thanh Xuan District, Hanoi, Vietnam
  • Nguyen Thi My Le Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, E2 Building, 144 Xuan Thuy Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Duy Toi Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, E2 Building, 144 Xuan Thuy Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Thi Van Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, E2 Building, 144 Xuan Thuy Road, Cau Giay District, Hanoi, Vietnam
  • Dinh Thuy Hang Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, E2 Building, 144 Xuan Thuy Road, Cau Giay District, Hanoi, Vietnam

DOI:

https://doi.org/10.15625/1811-4989/17070

Abstract

Nitrogen-fixing microorganisms (diazotrophs) converting the atmospheric N2 into usable form NH4 are considered the key players in the nitrogen cycle, chiefly responsible for the enriching nitrogen content in the soils. Globally, biological fixation of N2 greatly contributes to plant growth, lessens the need for chemical fertilizers, and thus contributes to the mitigation of greenhouse gases NOx. In this study, diazotrophic bacterial strains were isolated from rhizosphere soils and root nodules of legume and non-legume plants in Vietnam. Quantitative analyzes by the acetylene reduction assay showed that the isolates have high nitrogen fixation activity compared with that of reference strain Azospirillum vinelandii KCTC 2426. In addition, other effective capabilities of the isolated strains toward supporting agriculture were investigated, i.e. synthesizing IAA and siderophore for promoting plant growth, or producing exopolysaccharides for maintaining soil moisture. Taxonomic positions of the isolated strains were identified based on the comparative analyses of sequences of the 16S rDNA and gene related to nitrogen fixation (nifH), revealing a high taxonomic diversity among free-living and symbiotic diazotrophic isolates. Direct support of the selected isolates to plant growth was proven in experiments with mung beans under laboratory conditions. Thus, the native diazotrophic strains obtained in this study would be good microbial sources for application in organic agriculture and soil amendment.

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Published

28-12-2022

How to Cite

Thi Hieu Thu, N., Cao Son, T., Thu Trang, D., Thi My Le, N., Duy Toi, N., Thi Van, N., & Thuy Hang, D. (2022). Indigenous diazotrophs and their effective properties for organic agriculture. Vietnam Journal of Biotechnology, 20(4), 751–760. https://doi.org/10.15625/1811-4989/17070

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